1887

Abstract

SUMMARY

Intracytoplasmic type A particles known to be precursors to type B retroviruses in murine, hamster and marsupial cells are closely associated with microtubules and microtubule organizing centres. In this publication, the active participation of microtubules in the intracellular transport of the particles to the cell surface has been examined in NIH 3T3 cells infected with M432 virus using vincristine sulphate (VCR) as inhibitor of microtubule polymerization. The release of virus at different times after exposure to VCR was quantified by reverse transcriptase determinations of cell supernatants and by electron microscopic quantification of the number of virions at the cell surface using freeze-dried whole cell replicas. These studies indicate that VCR inhibits both microtubule polymerization and virus release, and thus suggest that intact cytoplasmic microtubules are necessary for intracellular transport and release of virus.

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1985-02-01
2024-04-28
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References

  1. Ainsworth S. K., Karnovsky M. J. 1972; An ultrastructural staining method for enhancing the size and electronopacity of ferritin in thin sections. Journal of Histochemistry and Cytochemistry 20:225–229
    [Google Scholar]
  2. Albrecht-Buehler G., Bushnell A. 1980; The ultrastructure of primary cilia in quiescent 3T3 cells. Experimental Cell Research 126:427–437
    [Google Scholar]
  3. Bensch K. G., Malawista S. E. 1969; Microtubular crystals in mammalian cells. Journal of Cell Biology 40:95–107
    [Google Scholar]
  4. Bernhard W. 1958; Electron microscopy of tumor cells and tumor viruses. Cancer Research 18:491–509
    [Google Scholar]
  5. Calafat J., Hilkens J. G. W. 1978; Ultrastructural study of virus-like particles in Chinese hamster lung cells. Journal of General Virology 41:417–420
    [Google Scholar]
  6. Callahan R., Benveniste R. E., Sherr C. J., Schidlovsky G., Todaro G. J. 1976; A new class of geneticallytransmitted retrovirus isolated from Mus cervicolor. Proceedings of the National Academy of Sciences, U.S.A 73:3579–3583
    [Google Scholar]
  7. Callahan R., Sherr C. J., Todaro G. J. 1977; A new class of murine retrovirus: immunological andbiochemical comparison of novel isolates from Mus cervicolor and Mus caroli. Virology 80:401–416
    [Google Scholar]
  8. Dales S., Chardonnet Y. 1973; Early events in the interaction of adenoviruses with HeLa cells. IV.Association with microtubules and the nuclear pore complex during vectorial movement of the inoculum. Virology 56:465–483
    [Google Scholar]
  9. Demsey A., Kawka D., Stackpole C. W. 1977; Application of freeze-drying intact cells to studies of murineoncornavirus morphogenesis. Journal of Virology 21:358–365
    [Google Scholar]
  10. Freed J. J., Liebowitz M. M. 1970; The association of a class of saltatory movements with microtubules incultured cells. Journal of Cell Biology 45:334–354
    [Google Scholar]
  11. Gould R. R., Borisy G. G. 1976; Discrete particles at the centrosomes and kinetochores of Chinese hamsterovary cells. Journal of Cell Biology 70:43a
    [Google Scholar]
  12. Hamilton R. C., MacGregor A., Pye D. 1979; A marsupial oncovirus?. Journal of General Virology 44:535–539
    [Google Scholar]
  13. Heine U. I. 1969; Electron microscopic studies on HeLa cells exposed to the antibiotic toyocamycin. CancerResearch 29:1875–1880
    [Google Scholar]
  14. Heine U. I., Todaro G. J. 1978; New type B retrovirus isolates associated with kinetochores and centriolesof thehost cell. Journal of General Virology 39:41–52
    [Google Scholar]
  15. Heine U. I., Kramarsky B., Wendel E., Suskind R. G. 1979a; Enhanced proliferation of endogenous virus inChinese hamster cells associated with microtubules and the mitotic apparatus of the host cell. Journal ofGeneral Virology 44:45–55
    [Google Scholar]
  16. Heine U. I., Margulies I., Demsey A. E., Suskind R. G. 1979b; Quantitative electron microscopy of intra-cytoplasmic type A particles at kinetochores of metaphase chromosomes isolated from Chinese hamster andmurine cell lines. Journal of General Virology 45:631–640
    [Google Scholar]
  17. Heine U. I., Suskind R. G., Margulies I., Demsey A. E. 1980; Oncornavirus precursor particles and the micro-tubule organizing centers. ArchivfürGeschwulstforschung 50:715–723
    [Google Scholar]
  18. MacGregor H. C., Stebbings H. 1970; A massive system of microtubules associated with cytoplasmic movement in telotrophic ovarioles. Journal of Cell Science 6:431–449
    [Google Scholar]
  19. Murti K. G., Goorha R. 1983; Interaction of frog virus-3 with the cytoskeleton. I. Altered organization ofmicrotubules, intermediate filaments, and microfilaments. Journal of Cell Biology 96:1248–1257
    [Google Scholar]
  20. Pepper D. A., Brinkley B. R. 1977; Localization of tubulin in the mitotic apparatus of mammalian cells byimmunofluorescence and immunoelectron microscopy. Chromosoma 60:223–235
    [Google Scholar]
  21. Porter K. R. 1973; Microtubules in intracellular locomotion. In Locomotion of Tissue Cells (Ciba FoundationSymposium 14), p 149 Amsterdam: Excerpta Medica;
    [Google Scholar]
  22. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  23. Satake M., Luftig R. B. 1982; Microtubule-depolymerizing agents inhibit Moloney murine leukaemia virusproduction. Journal of General Virology 58:339–349
    [Google Scholar]
  24. Sharpe A. H., Chen L. B., Murphy J. R., Fields B. N. 1982; Specific disruption of vimentin filamentorganization in monkey kidney CV-1 cells by diphtheria toxin, exotoxin A, and cycloheximide. Proceedings of the National Academy of Sciences, U.S.A 77:7267–7271
    [Google Scholar]
  25. Snyder J. A., McIntosh J. R. 1976; Studies on the centriolar region of mammalian cells at the onset of mitosis. Journal of Cell Biology 70:368a
    [Google Scholar]
  26. Stanislawski L. 1983; Localization with the electron microscope of p27 Rous sarcoma virus internal protein inchicken embryo fibroblasts. Intervirology 20:8–18
    [Google Scholar]
  27. Travis J. L., Allen R. D. 1981; Studies on the motility of the foraminifera. I. Ultrastructure of the reticulopodial network of Allogromialaticollaris (Arnold). Journal of Cell Biology 90:211–221
    [Google Scholar]
  28. Wheatley D. N. 1974; Pericentriolar virus-like particles in Chinese hamster ovary cells. Journal of General Virology 24:395–399
    [Google Scholar]
  29. Willingham M. C., Yamada S. S. 1979; Development of a new primary fixative for electron microscopicimmunocytochemical localization of intracellular antigens in cultured cells. Journal of Histochemistry and Cytochemistry 27:947–960
    [Google Scholar]
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