1887

Abstract

SUMMARY

cleavage of Gazdar murine sarcoma virus Pr65, which has all of the antigenic determinants of Moloney murine leukaemia virus Pr65, i.e. p15, p12, p30 and p10, by the Moloney murine leukaemia virus proteolytic activity yielded a p30 whose partial NH-terminal sequence was identical to Moloney murine leukaemia virus. Both [H]leucine-labelled and unlabelled Pr65 were used to generate the cleaved p30.

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1985-02-01
2024-04-27
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References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Copeland T. D., Grandgenett D. P., Oroszlan S. 1980; Amino acid sequence analysis of reverse transcriptase subunits from avian myeloblastosis virus. Journal of Virology 36:115–119
    [Google Scholar]
  3. Henderson L. E., Copeland T. D., Oroszlan S. 1980; Separation of all amino acid phenyl thiohydantoins by high performance liquid chromatography on phenylalkyl support. Analytical Biochemistry 102:1–7
    [Google Scholar]
  4. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  5. Lu A. J., Soong M. M., Wong P. F. Y. 1979; Maturation of Moloney murine leukemia virus. Virology 93:269–274
    [Google Scholar]
  6. Maxwell S., Arlinghaus R. B. 1981; In vitro proteolytic cleavage of Gazdar murine sarcoma virus p65gag. Journal of Virology 39:963–967
    [Google Scholar]
  7. Oroszlan S., Gilden R. V. 1980; Primary structure analysis of retrovirus proteins. In Molecular Biology of RNA Tumor Viruses pp 299–394 Edited by Stephenson J. R. New York: Academic Press;
    [Google Scholar]
  8. Shinnick T. M., Lerner R. A., Sutcliffe J. G. 1981; Nucleotide sequence of Moloney murine leukemia virus. Nature, London 293:543–548
    [Google Scholar]
  9. Syrewicz I. J., Naso R. B., Wang C. S., Arlinghaus R. B. 1972; Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle culture. Applied Microbiology 24:448–498
    [Google Scholar]
  10. Witte O. N., Baltimore D. 1978; Relationship of retrovirus polyprotein cleavages to virion maturation studied with temperature sensitive murine leukemia virus mutants. Journal of Virology 26:750–761
    [Google Scholar]
  11. Yoshinaka Y., Luftig R. B. 1977; Murine leukemia virus morphogenesis: cleavage of p70 in vitro can be accompanied by a shift from a concentrically coiled internal strand (‘immature’) to a collapsed (‘mature’) form of the virus core. Proceedings of the National Academy of Sciences, U.S.A 74:3446–3450
    [Google Scholar]
  12. Yoshinaka Y., Luftig R. B. 1980; Physicochemical characterization and specificity of the murine leukaemia virus Pr65gag proteolytic factor. Journal of General Virology 48:329–340
    [Google Scholar]
  13. Yoshinaka Y., Luftig R. B. 1982; p65 of Gazdar murine sarcoma viruses contains antigenic determinants from all four of the murine leukemia virus (MuLV) gag polypeptides (p15, p12, p30, p10) and can be cleaved in vitro by the MuLV proteolytic activity. Virology 118:380–388
    [Google Scholar]
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