1887

Abstract

SUMMARY

A topoisomerase activity is associated with herpes simplex virus type 1. The enzyme was recovered from purified virions which were disrupted with 6 -guanidine-HC1 followed by renaturation of extracted proteins. Based upon the following observations, the virion activity is classified as a type I topoisomerase: (i) the linking number of a unique DNA topoisomer is altered in steps of one; (ii) ATP and MgCl are not required for activity; (iii) the enzyme can be trapped in a covalent complex with DNA; (iv) the covalent linkage to DNA is through a 3′ phosphoryl bond. A number of lines of evidence strongly indicate that the topoisomerase is external to the nucleocapsid. For example, the activity was released by treatment of intact virions with NP40, and subsequent washing steps extracted most residual activity. When guanidine extracts were prepared from nucleocapsids, topoisomerase activity was not detectable. Finally, DNA within the virion did not appear to contain covalently attached proteins with properties similar to topoisomerases. Thus, the enzyme appears to be a component of the envelope or tegument structure of the virion.

Keyword(s): HSV-1 and topoisomerase type I
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1985-07-01
2024-05-18
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References

  1. Akrigg A., Cook P. R. 1980; DNA gyrase stimulates transcription. Nucleic Acids Research 8:845–854
    [Google Scholar]
  2. Batterson W., Roizman B. 1983; Characterization of the herpes simplex virion-associated factor responsible for the induction of a genes. Journal of Virology 46:371–377
    [Google Scholar]
  3. Bauer R. W., Ressner E. C., Kates J., Patzle J. V. 1977; A DNA nicking and closing enzyme encapsidated in vaccinia virus: partial purification and properties. Proceedings of the National Academy of Sciences, U.S.A 74:1841–1845
    [Google Scholar]
  4. Been M. D., Champoux E. J. 1981; DNA breakage and closure by rat liver type I topoisomerase: separation of the half reactions by using a single-stranded DNA substrate. Proceedings of the National Academy of Sciences, U.S.A 78:2883–2887
    [Google Scholar]
  5. Biswal N., Feldan P., Levy C. C. 1983; A DNA topoisomerase activity co-purifies with the DNA polymerase induced by herpes simplex virus. Biochimica et biophysica acta 740:379–389
    [Google Scholar]
  6. Brown P. O., Cozzarelli N. R. 1981; Catenation and knotting of duplex DNA by type 1 topoisomerases: a mechanistic parallel with type 2 topoisomerases. Proceedings of the National Academy of Sciences, U.S.A 78:843–847
    [Google Scholar]
  7. Campbell M. E. M., Palfreyman J. W., Preston C. M. 1984; Identification of herpes simplex virus DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcription. Journal of Molecular Biology 180:1–19
    [Google Scholar]
  8. Champoux J. J. 1976; Evidence for an intermediate with a single-strand break in the reaction catalyzed by the DNA untwisting enzyme. Proceedings of I he National Academy of Sciences, U.S.A 73:3488–3491
    [Google Scholar]
  9. Champoux J. J. 1978; Proteins that affect DNA conformation. Annual Review of Biochemistry 47:449–479
    [Google Scholar]
  10. Cordingley M. G., Campbell M. E. M., Preston C. M. 1983; Functional analysis of a herpes simplex virus type I promoter: identification of far-upstream regulatory sequences. Nucleic Acids Research 11:2347–2365
    [Google Scholar]
  11. Crick F. H. C. 1976; Linking numbers and nucleosomes. Proceedings of the National Academy of Sciences, U.S.A 73:2639–2643
    [Google Scholar]
  12. Davison A. J., Wilkie N. M. 1983; Inversion of the two segments of the herpes simplex virus genome in intertypic recombinants. Journal of General Virology 64:1–18
    [Google Scholar]
  13. Dinardo S., Voelkel K., Sternglanz R. 1984; DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at termination of DNA replication. Proceedings of the National Academy of Sciences, U.S.A 81:2616–2620
    [Google Scholar]
  14. Edwards K. A., Halligan B. D., Davis J. L., Nivera N. L., Liu L. F. 1980; Recognition sites of eukaryotic topoisomerase I: DNA nucleotide sequencing analysis of topo I cleavage sites on SV40 DNA. Nucleic Acids Research 10:2565–2576
    [Google Scholar]
  15. Gellert M. 1981; DNA topoisomerases. Annual Review of Biochemistry 50:879–910
    [Google Scholar]
  16. Hager D. A., Burgess R. R. 1980; Elution of proteins from SDS polyacrylamide gels, removal of SDS and renaturation of enzymatic activity, results with sigma subunit of E. coli RNA polymerase, wheat germ topoisomerase and other enzymes. Analytical Biochemistry 109:76–83
    [Google Scholar]
  17. Halligan B. D., Davis J. L., Edwards K. A., Liu L. F. 1982; Intra- and intermolecular strand transfer by eukaryotic DNA topoisomerase I. Journal of Biological Chemistry 257:3995–4000
    [Google Scholar]
  18. Jacob R. J., Morse L. S., Roizman B. 1979; Anatomy of herpes simplex virus DNA. XII. Accumulation of head-to-tail concatemers in nuclei of infected cells and their role in the generation of the four isomeric arrangements of viral DNA. Journal of Virology 29:448–457
    [Google Scholar]
  19. James C. D., Leffak I. M. 1984; Replacement synthesis labeling of DNA molecules in vitro using the Escherichia coli exonuclease III/DNA polymerase I enzyme pair. Analytical Biochemistry 141:33–37
    [Google Scholar]
  20. Kikuchi Y., Nash H. A. 1979; Nicking and closing activity associated with bacteriophage X INT gene product. Proceedings of the National Academy of Sciences, U.S.A 76:3760–3764
    [Google Scholar]
  21. Leary K., Francke B. 1984; The interaction of a topoisomerase-like enzyme from herpes simplex virus type 1-infected cells with non-viral circular DNA. Journal of General Virology 65:1341–1350
    [Google Scholar]
  22. Liu L. F. 1983; DNA topoisomerases: enzymes that catalyze the breaking and rejoining of DNA. CRC Reviews in Biochemistry 15:1–24
    [Google Scholar]
  23. Liu L. F., Liu C. C., Alberts B. M. 1980; Type II topoisomerases: enzymes that can unknot a topologically knotted DNA molecule via a reversible double-stranded break. Cell 19:697–707
    [Google Scholar]
  24. Mackem S., Roizman B. 1982; Structural features of the herpes simplex virus a gene 4, 0, and 27 promoter-regulatory sequences which confer a regulation of chimeric thymidine kinase genes. Journal of Virology 44:939–949
    [Google Scholar]
  25. Marini J. C., Miller K. G., Englund P. T. 1980; Decatenation of kinetoplast DNA by topoisomerases. Journal of Biological Chemistry 255:4976–4979
    [Google Scholar]
  26. Parris D. S., Courtney R. J., Schaffer P. A. 1978; Temperature-sensitive mutants of herpes simplex virus type I defective in transcriptional and post-transcriptional functions required for viral DNA synthesis. Virology 90:177–186
    [Google Scholar]
  27. Parris D. S., Dixon R. A. F., Schaffer P. A. 1980; Physical mapping of herpes simplex virus type I ts mutants by marker rescue: correlation of the physical and genetic maps. Virology 100:275–287
    [Google Scholar]
  28. Poffenberger K. L., Roizman B. 1985; A noninverting genome of a viable herpes simplex virus I: presence of head-to-tail linkages in packaged genomes and requirements for circularization after infection. Journal of Virology 53:587–595
    [Google Scholar]
  29. Post L. E., Mackem S., Roizman B. 1981; Regulation of genes by herpes simplex virus: expression of chimeric genes produced by fusion of thymidine kinase with a gene promoters. Cell 24:555–565
    [Google Scholar]
  30. Prell B., Vosberg H.-P. 1980; Analysis of covalent complexes formed between calf thymus DNA topoisomerase and single-stranded DNA. European Journal of Biochemistry 108:389–398
    [Google Scholar]
  31. Preston C. M., Cordingley M. G., Stow N. D. 1984; Analysis of DNA sequences which regulate the transcription of a herpes simplex virus immediate early gene. Journal of Virology 50:708–716
    [Google Scholar]
  32. Roizman B., Furlong D. 1974; The replication of herpes virus. In Comprehensive Virology vol 3: pp 209–403 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  33. Sander M., Hsieh T. 1983; Double strand DNA cleavage by type II DNA topoisomerase from Drosophila melanogaster. Journal of Biological Chemistry 285:8421–8428
    [Google Scholar]
  34. Smith K. O. 1964; Relationship between the envelope and infectivity of herpes simplex virus. Proceedings of the Society for Experimental Biology and Medicine 115:814–816
    [Google Scholar]
  35. Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. Journal of Virology 9:143–159
    [Google Scholar]
  36. Sternglanz R., Dinardo S., Voelkel K. A., Nishimura Y., Hirota Y., Becherer K., Zumstein L., Wang J. C. 1981; Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition. Proceedings of the National Academy of Sciences, U.S.A 78:2747–2751
    [Google Scholar]
  37. Trask D. K., Muller M. T. 1983; Biochemical characterization of topoisomerase I purified from avian erythrocytes. Nucleic Acids Research 11:2779–2800
    [Google Scholar]
  38. Trask D. K., Didonato J. A., Muller M. T. 1984; Rapid detection and isolation of covalent DNA/protein complexes: application to topoisomerase I and II. EMBO Journal 3:671–676
    [Google Scholar]
  39. Tsubota Y., Waqar M. A., Burke J. F., Milavetz B. I., Evans M. J., Kowalski D., Huberman J. A. 1978; Association of enzymes with replicating and non-replicating simian virus 40 chromosomes. Cold Spring Harbor Symposia on Quantitative Biology 43:693–704
    [Google Scholar]
  40. Weisbrod S. T. 1982; Properties of active nucleosomes as revealed by HMG 14 and 17 chromatography. Nucleic Acids Research 10:2017–2036
    [Google Scholar]
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