1887

Abstract

Summary

Using a murine monoclonal antibody (RF-HBs-1) which has been shown to be capable of neutralizing both and subtypes of hepatitis B virus (HBV), we have devised a competitive inhibition assay to measure the presence of virus-neutralizing antibodies in the sera of patients who have recovered from acute type B hepatitis. The majority of patients have this antibody in their serum. We also show that this antibody inhibits the binding of polymerized human serum albumin (pHSA) to the pHSA receptor site of the HBV particle, which has been proposed as an important site for the entry of HBV into liver cells. We have demonstrated that the epitope recognized by this antibody is dependent on the linkage of 24000 and 28000 mol. wt. polypeptides via a disulphide bond. This conformational determinant in the coat of the virus which is part of or near to the pHSA binding site is important in evoking a virus-neutralizing response.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-11-2467
1986-11-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/11/JV0670112467.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-11-2467&mimeType=html&fmt=ahah

References

  1. Cabral G. A., Marciano-Cabral F., Funk G. A., Sanchez Y., Hollinger F. B., Melnick J. L., Dreesman G.R. 1978; Cellular and humoral immunity in guinea pigs to two major polypeptides derived from hepatitis B surface antigen. Journal of General Virology 38:339–350
    [Google Scholar]
  2. Dreesman G. R., Hollinger F. B., Melnick J. L. 1975; Biophysical and biochemical properties of purified preparations of hepatitis B surface antigen (HBsAg). American Journal of Medical Science 270:123–129
    [Google Scholar]
  3. Goodall A. H., Miescher G., Meek F. L., Janossy G., Thomas H.C. 1981; Monoclonal antibodies in a solid-phase radiometric assay for HBsAg. Medical Laboratory Sciences 38:349–354
    [Google Scholar]
  4. Hansson B. G., Purcell R. H. 1979; Sites that bind polymerised albumin on hepatitis B surface antigen particles: detection by radioimmunoassay. Infection and Immunity 26:125–130
    [Google Scholar]
  5. Imai M., Yanase Y., Nojiri T., Niyakawa Y., Mayumi M. 1979; A receptor for polymerised human and chimpanzee albumins on hepatitis B virus particles co-occurring with HBeAg. Gastroenterology 76:242–247
    [Google Scholar]
  6. Ionescu-Matiu I., Sandez Y., Hollinger F. B. 1980; Presence of a receptor for albumin on individual HBsAg polypeptides and on HBsAg produced by a hepatoma cell line. Journal of Medical Virology 6:175–178
    [Google Scholar]
  7. Iwarson S., Tabor E., Thomas H. C., Goodall A., Waters J., Snoy P., Wai-Kuo Shih J., Gerety R. J. 1985; Neutralisation of hepatitis B virus infectivity by a murine monoclonal antibody: an experimental study in the chimpanzee. Journal of Medical Virology 16:89–95
    [Google Scholar]
  8. Jilg W., Schmidt M., Zoulek G., Larbeer B., Wilske B., Deinhardt F. 1984; Clinical evaluation of a recombinant hepatitis B vaccine. Lancet ii:1174–1175
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Lenkei R., Onica D., Ghetie B. 1977; Receptors for polymerised albumin on liver cells. Experientia 33:1046–1047
    [Google Scholar]
  11. Machida A., Kishimoto S., Ohnuma H., Miyamoto H., Baba K., Oda K., Nakamuna T., Miyakawa Y., Mayumi M. 1983; A hepatitis B surface antigen polypeptide (p31) with the receptor for polymerized human as well as chimpanzee albumins. Gastroenterology 85:268–274
    [Google Scholar]
  12. Melnick J. L. 1981; Historical aspects of hepatitis B vaccine. In Hepatitis B Vaccine pp 23–31 Edited by Maupas P., Guesry P. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  13. Mishuiro S., Imai M., Takahashi K., Machido A., Gotanda T., Miyakawa Y., Mayumi M. 1980; A 49000 dalton polypeptide bearing all antigenic determinants and full immunogenicity of 22 nm hepatitis B surface antigen particles. Journal of Immunology 124:1589–1593
    [Google Scholar]
  14. Neurath A. R., Kent S. B. H., Strick N. 1984; Location and chemical synthesis of a pre-S gene coded immunodominant epitope of hepatitis B virus. Science 224:392–395
    [Google Scholar]
  15. Peterson D. L. 1981; Isolation and characterisation of the major protein and glycoprotein of hepatitis B surface antigen. Journal of Biological Chemistry 256:6975–6983
    [Google Scholar]
  16. Renast I., Reiser J., Stark G. R. 1979; Transfer of proteins from gels to diazobenzyloxymethyl paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proceedings of the National Academy of Sciences, U.S.A. 76:3116–3120
    [Google Scholar]
  17. Skelly J., Howard C. R., Zuckerman A. J. 1981; Hepatitis B polypeptide vaccine preparation in micelle form. Nature, London 290:51–54
    [Google Scholar]
  18. Stibbe W., Gerlich W. H. 1982; Variable protein composition of hepatitis B surface antigen from different donors. Virology 123:436–442
    [Google Scholar]
  19. Szmuness W., Stevens C. D., Harley E. J., Zang E. A., Oleszko W. R., William D. C., Sadovsky R., Morrison J. M., Kellner A. 1980; Hepatitis B vaccine. Demonstration of efficacy in a controlled clinical trial in a high-risk population in the United States. New England Journal of Medicine 303:833–841
    [Google Scholar]
  20. Szmuness W., Stevens C. E., Harley E. J. 1982; Hepatitis B vaccine in medical staff of haemodialysis units: efficacy and subtype cross-protection. New England Journal of Medicine 307:1481–1486
    [Google Scholar]
  21. Trevisan A., Gudat F., Guggenheim R., Krey G., Durmuller V., Luond G., Duggelin M., Landmann I., Tondelu P., Bianchi L. 1982; Demonstration of albumin receptors on isolated human hepatocytes by light and scanning electron microscopy. Hepatology 2:832
    [Google Scholar]
  22. Zuckerman A. J., Howard C. R. 1979 Hepatitis Viruses of Man London: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-11-2467
Loading
/content/journal/jgv/10.1099/0022-1317-67-11-2467
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error