1887

Abstract

Summary

A lapinized rinderpest virus, the L strain, which is virulent in rabbits and had been grown only in rabbits, was adapted to grow in Vero cells by the fusion of Vero cells with virus-infected rabbit spleen cells in the presence of polyethylene glycol, and subsequently passaged in Vero cells by co-culture technique. After several passages, free virus was produced at high titre. The Vero cell-adapted virus acquired the ability to infect several cell lines which were non-permissive to the unadapted virus. Analysis of virus proteins by immunofluorescence using monoclonal antibodies revealed that marked changes occurred in F, P, NP and M proteins by passage in Vero cells. In parallel to the adaptation to cell culture , the virulence of the virus measured in terms of clinical signs and histological lesions in the lymphoid tissues decreased in its severity whereas its immunosuppressive capacity was maintained unaltered. Thus, rinderpest virus with different degrees of virulence is now available for study .

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/content/journal/jgv/10.1099/0022-1317-67-2-275
1986-02-01
2024-05-05
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References

  1. Fraser K. B., Martin S. J. 1978 Measles Virus and its Biology pp. 12–24 London: Academic Press;
    [Google Scholar]
  2. Fukuda A., Yamanouchi K. 1976; Autoimmunity induced in rabbits by rinderpest virus. Infection and Immunity 13:1449–1453
    [Google Scholar]
  3. Fukuda A., Yamanouchi K. 1981; Comparison of autoimmunity induction with virulent and attenuated rinderpest virus in rabbits. Japanese Journal of Medical Science and Biology 34:149–159
    [Google Scholar]
  4. Fukusho K., Nakamura J. 1940; On the experimental infection with rinderpest virus in rabbits. II. Pathology. Japanese Journal of Veterinary Science 2:75–101 (in Japanese)
    [Google Scholar]
  5. Homma M., Ohuchi M. 1973; Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural difference of Sendai viruses grown in eggs and tissue culture cells. Journal of Virology 12:1457–1465
    [Google Scholar]
  6. Katz M. 1977 Slow Virus Infection of the Central Nervous System pp. 106–115 Edited by Ter Meulen V. Katz M. New York: Springer-Verlag;
    [Google Scholar]
  7. Kobune F., Chino F., Yamanouchi K. 1976; Studies on recovery mechanism from rinderpest virus infection in rabbits. I. Effect of anti-thymocyte serum and thymectomy. Japanese Journal of Medical Science and Biology 29:265–275
    [Google Scholar]
  8. Nagai Y., Klenk H. D. 1977; Activation of precursors to both glycoproteins of Newcastle disease virus by proteolytic cleavage. Virology 77:125–134
    [Google Scholar]
  9. Nagai Y., Klenk H. D., Rott R. 1970; Proteolytic cleavage of viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508
    [Google Scholar]
  10. Nakamura J., Miyamoto T. 1953; Avianization of lapinized rinderpest virus. American Journal of Veterinary Research 14:307–317
    [Google Scholar]
  11. Rohde E., Pauli G., Henning J., Friis R. R. 1978; Polyethylene glycol-mediated infection with avian sarcoma viruses. Archives of Virology 58:55–59
    [Google Scholar]
  12. Sakaguchi M., Yoshikawa Y., Yamanouchi K. 1984; Growth of measles and subacute sclerosing panencephalitis viruses in human neural cell lines. Microbiology and Immunology 28:461–469
    [Google Scholar]
  13. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  14. Shishido A., Yamanouchi K., Hikita M., Sato T., Fukuda A., Kobune F. 1976; Development of a cell culture system susceptible to measles, canine distemper, and rinderpest viruses. Archiv für die gesamte Virusforschung 22:364–380
    [Google Scholar]
  15. Taniguchi H., Yoskikawa Y., Yamanouchi K. 1982; Response of lymphocytes of Japanese quails to mitogens. Japanese Journal of Veterinary Science 44:759–766
    [Google Scholar]
  16. Yamanouchi K., Chino F., Kobune F., Fukuda A., Yoshikawa Y. 1974a; Pathogenesis of rinderpest virus infection in rabbits. I. Clinical signs, immune response, histological changes, and virus growth patterns. Infection and Immunity 9:199–205
    [Google Scholar]
  17. Yamanouchi K., Fukuda A., Kobune F., Yoshikawa Y., Chino F. 1974b; Pathogenesis of rinderpest virus infection in rabbits. II. Effect of rinderpest virus on the immune functions of rabbits. Infection and Immunity 9:206–211
    [Google Scholar]
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