1887

Journal of General Virology header logo

Volume 67, Issue 2

Viable Viruses with Deletions in the Left Inverted Terminal Repeat Define the Adenovirus Origin of DNA Replication Free

Abstract

Summary

A series of human adenovirus type 2 genomes with deletions in the left inverted terminal repeat (ITR) have been constructed. Viral genomes that contained a minimum of 45 base pairs (bp) from the terminus of the genome were fully infectious and gave rise to progeny virus which maintained the deletion. In contrast, genomes containing 36 bp or less from the termini of the genome were not infectious. The boundary of a -acting element required for viral replication is therefore between 36 and 45 bp from the adenovirus termini and corresponds to the previously identified viral origin of replication, defined using a transfection assay to measure activity . The growth parameters of viruses with deletions in the left ITR were examined. These deletions had no measurable effect on plaque formation or morphology, viral DNA synthesis or early viral mRNA synthesis. Thus, it appears that DNA sequences in the left ITR, outside the replication origin, are completely dispensable for lytic viral growth in tissue culture cells.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): adenovirus , ITR , mutants and replication
© Society for General Microbiology 1986
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-2-321
1986-02-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/2/JV0670020321.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-2-321&mimeType=html&fmt=ahah

References

  1. Alestrom P., Stenlund A., Pettersson U. 1982; A common sequence in the inverted terminal repetitions of human and avian adenoviruses. Gene 18:193–197
     [Google Scholar]
  2. Alwine J. C., Kemp D. J., Stark G. R. 1977; Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proceedings of the National Academy of Sciences, U.S.A 74:5350–5354
     [Google Scholar]
  3. Arrand J. R., Roberts R. J. 1979; The nucleotide sequence at the termini of adenovirus 2 DNA. Journal of Molecular Biology 128:577–594
     [Google Scholar]
  4. Barrett T., Mahy B. W. J. 1984; Molecular cloning of the nucleoprotein gene of canine distemper virus. Journal of General Virology 65:549–557
     [Google Scholar]
  5. Borgmeyer U., Nowock J., Sippel A. E. 1984; The TGGCA-binding protein: a eukaryotic nuclear protein recognizing a symmetrical sequence on double-stranded linear DNA. Nucleic Acids Research 10:4295–4311
     [Google Scholar]
  6. Carusi E. A. 1977; Evidence for blocked 5′-termini in human adenovirus DNA. Virology 76:380–394
     [Google Scholar]
  7. Challberg M. D., Rawlins D. R. 1984; Template requirements for the initiation of adenovirus DNA replication. Proceedings of the National Academy of Sciences, U.S.A 81:100–104
     [Google Scholar]
  8. Church G. M., Gilbert W. 1984; Genomic sequencing. Proceedings of the National Academy of Sciences, U,. S,. A. 81:1991–1995
     [Google Scholar]
  9. Cole C. M., Landers T., Goff S. P., Manteuil-Brutlag S., Berg P. 1977; Physical and genetic characterization of deletion mutants of SV40 constructed in vitro. Journal of Virology 24:277–294
     [Google Scholar]
  10. Daniell E. 1976; Genome structure of incomplete particles of adenovirus. Journal of Virology 19:685–708
     [Google Scholar]
  11. Desiderio S. V., Kelly T. J. 1981; Structure of the linkage between adenovirus DNA and the 55, 000 molecular weight terminal protein. Journal of Molecular Biology 145:319–337
     [Google Scholar]
  12. De Vries E., Van Driel W., Tromp M., Van Boom J., Van Der Vliet P. C. 1985; Adenovirus DNA replication in vitro: site directed mutagenesis of the nuclear factor I binding site of the Ad2 origin. Nucleic Acids Research 13:4935–4952
     [Google Scholar]
  13. Dimaio D., Nathans D. 1980; Cold sensitive regulatory mutants of SV40. Journal of Molecular Biology 140:129142
     [Google Scholar]
  14. Everett R. D. 1984; Transactivation of transcription by herpes virus products: requirement for two HSV-1 immediate early polypeptides for maximum activity. EMBO Journal 3:3135–3141
     [Google Scholar]
  15. Graham F. L., Smiley J., Russell W. C., Nairn R. 1977; Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36:59–72
     [Google Scholar]
  16. Gronostajski R. M., Adhya S., Nagata K., Guggenheimer R. A., Hurwitz J. 1985; Site-specific DNA binding of nuclear factor I: analysis of cellular binding sites. Molecular and Cellular Biology 5:964–971
     [Google Scholar]
  17. Guggenheimer R. A., Stillman B. W., Nagata K., Tamanoi F., Hurwitz J. 1984; DNA sequences required for the in vitro replication of adenovirus DNA. Proceedings of the National Academy of Sciences, U.S.A. 81:3069–3073
     [Google Scholar]
  18. Hay R. T. 1985a; The origin of adenovirus DNA replication: minimal DNA sequence requirement in vivo. EMBO Journal 4:421–426
     [Google Scholar]
  19. Hay R. T. 1985b; The origin of adenovirus DNA replication: role of the nuclear factor I binding site in vivo.. Journal of Molecular Biology (in press)
    [Google Scholar]
  20. Hay R. T., Depamphilis M. L. 1982; Initiation of SV40 DNA replication in vivo: location and structure of 5′ ends of DNA synthesized in the ori region. Cell 28:767–779
     [Google Scholar]
  21. Hay R. T., Stow N. D., Mcdougall I. M. 1984a; Replication of adenovirus mini-chromosomes. Journal of Molecular Biology 175:493–510
     [Google Scholar]
  22. Hay R. T., Hendrickson E. A., Depamphilis M. L. 1984b; Sequence specificity for the initiation of RNA-primed simian virus 40 DNA synthesis in vivo. Journal of Molecular Biology 175:131–157
     [Google Scholar]
  23. Hearing P., Shenk T. 1983; The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element. Cell 33:695–703
     [Google Scholar]
  24. Hennighausen L., Siebenlist U., Danner D., Leder P., Rawlins D., Rosenfeld P., Kelly T. 1985; High-affinity binding site for a specific nuclear protein in the human IgM gene. Nature, London 314:289–292
     [Google Scholar]
  25. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
     [Google Scholar]
  26. Jones N., Shenk T. 1979; Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. Cell 17:683–689
     [Google Scholar]
  27. Lally C., Dorper T., Grocer W., Antoine G., Winnacker E.-L. 1984; A size analysis of the adenovirus replicon. EMBO Journal 3:333–337
     [Google Scholar]
  28. Lechner R. L., Kelly T. J. 1977; The structure of replicating adenovirus 2 DNA molecules. Cell 12:1007–1020
     [Google Scholar]
  29. Leegwater P. A. J., Van Driel W., Van Der Vliet P. C. 1985; Recognition site of nuclear factor I, a sequence- specific DNA-binding protein from HeLa cells that stimulates adenovirus DNA replication. EMBO Journal 4:1515–1521
     [Google Scholar]
  30. Lichy J. H., Field J., Horwitz M. S., Hurwitz J. 1982; Separation of the adenovirus terminal protein precursor from its associated DNA polymerase: role of both proteins in the initiation of adenovirus DNA replication. Proceedings of the National Academy of Sciences, U.S.A. 79:5225–5229
     [Google Scholar]
  31. Mautner V., Willcox H. N. A. 1974; Adenovirus antigens: a model system in mice for subunit vaccination. Journal of General Virology 25:325–336
     [Google Scholar]
  32. Nagata K., Guggenheimer R. A., Hurwitz J. 1983; Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA. Proceedings of the National Academy of Sciences, U.S.A 80:6177–6181
     [Google Scholar]
  33. Nowock J., Borgmeyer U., Puschel A. W., Rupp R. A. W., Sippel A. E. 1985; The TGGCA protein binds to the MMTV-LTR, the adenovirus origin of replication and the BK virus enhancer. Nucleic Acids Research 13:2045–2061
     [Google Scholar]
  34. Pettersson U., Sambrook J. 1973; Amount of viral DNA in the genome of cells transformed by adenovirus type 2. Journal of Molecular Biology 73:125–130
     [Google Scholar]
  35. Rawlins D. R., Rosenfeld P. J., Wides R. J., Challberg M. D., Kelly T. J. 1984; Structure and function of the adenovirus origin of replication. Cell 37:309–319
     [Google Scholar]
  36. Rekosh D. M. K., Russell W. C., Bellett A. J. D., Robinson A. J. 1977; Identification of a protein linked to the end of adenovirus DNA. Cell 11:283–295
     [Google Scholar]
  37. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
     [Google Scholar]
  38. Shenk T. 1978; Construction of a viable SV40 variant containing two functional origins of DNA replication. Cell 13:791–798
     [Google Scholar]
  39. Shinagawa M., Padmanabhan R. 1980; Comparative sequence analysis of the inverted terminal repetitions from different adenoviruses. Proceedings of the National Academy of Sciences, U.S.A 77:3831–3835
     [Google Scholar]
  40. Siebenlist U., Hennighausen L., Battey J., Leder P. 1984; Chromatin structure and protein binding in the putative regulatory region of the c-myc gene in Burkitt lymphoma. Cell 37:381–391
     [Google Scholar]
  41. Stillman B. W., Lewis J. B., Chow L. T., Mathews M. B., Smart J. E. 1981; Identification of the gene and mRNA for the adenovirus terminal protein precursor. Cell 23:497–508
     [Google Scholar]
  42. Stillman B. W., Topp W. C., Engler J. A. 1982a; Conserved sequences at the origin of adenovirus DNA replication. Journal of Virology 44:530–537
     [Google Scholar]
  43. Stillman B. W., Tamanoi F., Mathews M. B. 1982b; Purification of an adenovirus-coded DNA polymerase that is required for initiation of DNA replication. Cell 31:613–623
     [Google Scholar]
  44. Stow N. D. 1981; Cloning of a DNA fragment from the left-hand terminus of the adenovirus type 2 genome and its use in site-directed mutagenesis. Journal of Virology 37:171–180
     [Google Scholar]
  45. Stow N. D. 1982; The infectivity of adenovirus genomes lacking DNA sequences from their left-hand termini. Nucleic Acids Research 10:5105–5119
     [Google Scholar]
  46. Subramanian K. M., Shenk T. 1978; Definition of the boundaries of the origin of DNA replication in SV40. Nucleic Acids Research 5:3635–3642
     [Google Scholar]
  47. Tamanoi F., Stillman B. W. 1983; A specific DNA sequence is required for the initiation of adenovirus DNA replication in vitro. Proceedings of the National Academy of Sciences, U,. S,. A. 80:6446–6450
     [Google Scholar]
  48. Van bergen B. G. M., Van Der Ley P. A., Van Driel W, Van Mansfeld A. D. M., Van Der Vliet P. C. 1983; Replication of origin containing adenovirus DNA fragments that do not carry the terminal protein. Nucleic Acids Research 11:1975–1989
     [Google Scholar]
  49. Williams J. F. 1970; Enhancement of adenovirus plaque formation on HeLa cells by magnesium chloride. Journal of General Virology 9:251–255
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-2-321
Loading
Viable Viruses with Deletions in the Left Inverted Terminal Repeat Define the Adenovirus Origin of DNA Replication
J. Gen. Virol. 67, 321 (1986); https://doi.org/10.1099/0022-1317-67-2-321
/content/journal/jgv/10.1099/0022-1317-67-2-321
/content/journal/jgv/10.1099/0022-1317-67-2-321
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error