1887

Abstract

Summary

Sixteen virus isolates with similar biological characteristics were obtained from saltmarsh mosquitoes collected in south Texas in 1974. When compared antigenically, these and 13 other isolates from mosquitoes collected between 1972 and 1979 in west Texas, New Mexico, Louisiana, Colorado and North Dakota were shown to be related but not identical. Three distinct serotypes were determined: Bahia Grande (prototype strain TB4-1054), Reed Ranch (TB4-222) and Muir Springs (76V-23524). When examined by electron microscopy, these three viruses were shown to be rhabdoviruses. Structural analysis of the prototype strain of Bahia Grande virus from Texas revealed five proteins. Comparative oligonucleotide fingerprint maps showed 51 to 86% sharing of the large oligonucleotides between Bahia Grande virus (strain TB4-1054) and 11 other antigenically related isolates but not with Muir Springs virus (strain 76V-23524) an antigenically distinct isolate from mosquitoes collected in Colorado. A serological survey for antibody to Bahia Grande virus showed that humans, cattle, sheep, reptiles and wild mammals from south Texas had neutralizing antibodies to this virus.

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1986-06-01
2024-04-27
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References

  1. Casey H. L. 1967; Standardized diagnostic complement fixation method and adaptation to micro-test. In Public Health Monograph No 74 Washington, D.C.: U.S. Government Printing Office;
    [Google Scholar]
  2. Dewachter R., Fiers W. 1972; Preparative two-dimensional polyacrylamide gel electrophoresis of 32P-labeled RNA. Analytical Biochemistry 49:184–195
    [Google Scholar]
  3. Hilz H., Wiegers U., Adamietz P. 1975; Stimulation of proteinase action by denaturing agents: application to the degradation of masked proteins. European Journal of Biochemistry 56:103–108
    [Google Scholar]
  4. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  5. Lee Y. F., Kitamura N., Nomoto A., Wimmer E. 1979; Sequence studies of poliovirus RNA. IV. Nucleotide sequence complexities of poliovirus type 1, type 2 and two type 1 defective interfering particles RNAs, and fingerprint of the poliovirus type 3 genome. Journal of General Virology 44:311–322
    [Google Scholar]
  6. Lindsey H. S., Calisher C. H., Mathews J. H. 1976; Serum dilution neutralization test for California group virus identification and serology. Journal of Clinical Microbiology 4:503–510
    [Google Scholar]
  7. Obijeski J. F., Marchenko A. T., Bishop D. H. L., Cann B. W., Murphy F. A. 1974; Comparative electrophoretic analysis of the virus proteins of four rhabdoviruses. Journal of General Virology 22:21–33
    [Google Scholar]
  8. Obijeski J. F., Bishop D. H. L., Murphy F. A., Palmer E. L. 1976; Structural proteins of La Crosse virus. Journal of Virology 19:985–997
    [Google Scholar]
  9. Pedersen F. A., Haseltine W. A. 1980; Analysis of the genome of an endogenous, ecotropic retrovirus of the AKR strain of mice: micromethod for detailed characterization of high-molecular-weight RNA. Journal of Virology 33:349–365
    [Google Scholar]
  10. Tesh R. B., Travassos Da Rosa A. P. A., Travassos Da Rosa J. S. 1983; Antigenic relationships among rhabdoviruses infecting terrestrial vertebrates. Journal of General Virology 64:169–176
    [Google Scholar]
  11. Tikasingh E. S., Spence L., Downs W. G. 1966; The use of adjuvant and sarcoma 180 cells in the production of mouse hyperimmune ascitic fluids to arboviruses. American Journal of Tropical Medicine and Hygiene 15:219–226
    [Google Scholar]
  12. Trent D. W., Grant J. A. 1980; A comparison of New World alphaviruses in the western equine encephalomyelitis complex by immunochemical and oligonucleotide fingerprint techniques. Journal of General Virology 47:261–282
    [Google Scholar]
  13. Trent D. W., Grant J. A., Vorndam A. V., Monath T. P. 1981; Genetic heterogeneity among Saint Louis encephalitis virus isolates of different geographic origin. Virology 114:319–332
    [Google Scholar]
  14. Trent D. W., Grant J. A., Rosen L., Monath T. P. 1983; Genetic variation among dengue 2 viruses of different geographic origin. Virology 128271–284
    [Google Scholar]
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