1887

Abstract

Summary

A collection of Mov mouse strains, each carrying in their germ line a Moloney murine leukaemia virus (M-MuLV) proviral genome at a different chromosomal location, was used to study expression of endogenous retroviruses. No M-MuLV - specific RNA was detected in the non-viraemic Mov strains studied, indicating that less than two copies of RNA are transcribed per cell. Virus expression was seen in three viraemic Mov strains. In Mov-3 mice the provirus was activated shortly after birth, whereas proviruses in Mov-9 and Mov-14 were activated at different stages of embryogenesis. The results suggest that the chromosomal position influences proviral expression during development. The first appearance of virus particles was hot accompanied by detectable amounts of viral transcripts, suggesting that viraemia is a consequence of provirus activation in a small, as yet unidentified, population of cells, followed by virus spread and infection of susceptible cells.

Keyword(s): Mov mice , MuLV and proviral activation
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/content/journal/jgv/10.1099/0022-1317-68-11-2919
1987-11-01
2024-05-02
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References

  1. Barklis E., Mulligan R. C., Jaenisch R. 1986; Chromosomal position or virus mutation permits retrovirus expression in embryonal carcinoma cells. Cell 47:391–399
    [Google Scholar]
  2. Fiedler W., Nobis P., Jähner D., Jaenisch R. 1982; Differentiation and virus expression in Balb/Mo mice: endogenous Moloney leukemia virus is not activated in hematopoietic cells. Proceedings of the National Academy of Sciences, U.S.A 79:1874–1878
    [Google Scholar]
  3. Harbers K., Kuehn M., Delius H., Jaenisch R. 1984; Insertion of retrovirus into the first intron OF al(I) collagen gene leads to embryonic lethal mutation in mice. Proceedings of the National Academy of Sciences, U.S.A 81:1504–1508
    [Google Scholar]
  4. Jaenisch R. 1976; Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus. Proceedings of the National Academy of Sciences, U.S.A 73:1260–1264
    [Google Scholar]
  5. Jaenisch R., Jähner D., Nobis P., Simon I., Löhler J., Harbers K., Grotkopp D. 1981; Chromosomal position and activation of retroviral genomes inserted into the germ line of mice. Cell 24:519–529
    [Google Scholar]
  6. Jaenisch R., Schnieke A., Harbers K. 1985; Treatment of mice with 5-azacytidine efficiently activates silent retroviral genomes in different tissues. Proceedings of the National Academy of Sciences, U.S.A 82:1451–1455
    [Google Scholar]
  7. Jähner D., Jaenisch R. 1980; Integration of Moloneyleukaemia virus into the germ line of mice: correlation between genotype and virus activation. Nature; London: 287456–458
    [Google Scholar]
  8. Jähner D., Jaenisch R. 1984; DNA methylation in early mammalian development. In DNA Methylation pp. 189–219 Razin A., Cedar H., Riggs A. D. Edited by New York: Springer-Verlag;
    [Google Scholar]
  9. Jähner D., Haase K., Mulligan R. C. M., Jaenisch R. 1985; Insertion of the bacterial gpt gene into the germ line of mice by retroviral infection. Proceedings of the National Academy of Sciences, U.S.A 82:6927–6931
    [Google Scholar]
  10. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Münke M., Harbers K., Jaenisch R., Francke U. 1986; Chromosomal mapping of four different integration sites of Moloney murine leukemia virus including the locus for α1 (I) collagen in mouse. Cytogenetics and Cell Genetics 43:140–149
    [Google Scholar]
  12. Nobis P., Jaenisch R. 1980; Passive immunotherapy prevents expression of endogenous Moloney virus and amplification of proviral DNA in Balb/Mo mice. Proceedings of the National Academy of Sciences, U.S.A 77:3677–3681
    [Google Scholar]
  13. Rowe W. P., Pugh W. E., Hartley J. W. 1970; Plaque assay techniques for murine leukemia viruses. Virology 42:1136–1139
    [Google Scholar]
  14. Schnieke A., Stuhlmann H., Harbers K., Chumakov I., Jaenisch R. 1983; Endogenous Moloney leukemia virus in nonviremicMovsubstrains of mice carries defects in the proviral genome. Journal of Virology 45:505–513
    [Google Scholar]
  15. Shinnick T. M., Lerner R. A., Sutcliffe J. G. 1981; Nucleotide sequence of Moloney murine leukaemia virus. Nature; London: 293543–548
    [Google Scholar]
  16. Simon I., Löhler J., Jaenisch R. 1982; Virus-specific transcription and translation in organs of Balb/Mo mice: comparative study using quantitative hybridization, in situ hybridization and immunocytochemistry. Virology 120:106–121
    [Google Scholar]
  17. Soriano P., Jaenisch R. 1986; Retroviruses as probes for mammalian development: allocation of cells to the somatic and germ cell lineages. Cell 46:19–29
    [Google Scholar]
  18. Soriano P., Cone R. D., Mulligan R. C., Jaenisch R. 1986; Tissue specific and ectopic expression of genes introduced into transgenic mice by retroviruses. Science 234:1409–1413
    [Google Scholar]
  19. Stewart C., Harbers K., Jähner D., Jaenisch R. 1983; X-chromosome-linked transmission and expression of retroviral genomes microinjected into mouse zygotes. Science 221:760–762
    [Google Scholar]
  20. Weaver R. F., Weissmann C. 1979; Mapping of RNA by modification of the Berk-Sharp procedure: the 5′ prime termini of 15S β-globin mRNA precursor and mature 10S β-globm mRNA have identical map coordinates. Nucleic Acids Research 7:1175–1193
    [Google Scholar]
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