1887

Abstract

Summary

The growth of vesicular stomatitis virus (VSV) can be inhibited by the antiviral compound tricyclo-decane-9-yl-xanthogenate (D609). On analysing the antiviral mechanism we found no effect on the primary transcription of infecting VSV genomes.In contrast, the processes of replication and transcription during late stages of infection were inhibited. Despite the synthesis of all five virus-coded proteins (41 % to 56% of the uninhibited control), as shown by labelling with [S]methionine, the phosphorylation of the non-structural (NS) protein was reduced in the presence of the xanthate by a factor of at least 17. The pattern of phosphorylation of the bulk of cellular proteins remained unaltered under the same conditions. A relation between a possible loss of biological activity of the NS protein owing to the lack of phosphorylation and the decreased VSV RNA synthesis is suggested.

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1987-12-01
2024-04-30
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References

  1. Amtmann E., Sauer G. 1982; Bovine papilloma virus transcription: polyadenylated RNA species and assessment of the direction of transcription. Journal of Virology 43:59–66
    [Google Scholar]
  2. Amtmann E., Hummel K., Sauer G. 1985a; The therapeutic efficacy of a xanthate compound on herpes simplex virus in skin lesions of mice and guinea-pigs. Journal of General Virology 66:1953–1960
    [Google Scholar]
  3. Amtmann E., Müller K., Knapp A., Sauer G. 1985b; Reversion of bovine papillomavirus induced transformation and immortalization by a xanthate compound. Experimental Cell Research 161:541–550
    [Google Scholar]
  4. Amtmann E., Müller-Decker K., Hoss A., Schalasta G., Doppler C., Sauter G. 1987; Synergistic antiviral effect of xanthates and ionic detergents. Biochemical Pharmacology 36:1545–1549
    [Google Scholar]
  5. Arnheiter H., Davis N. L., Wertz G., Schubert M., Lazzarini R. A. 1985; Role of the nucleocapsid protein in regulating vesicular stomatitis virus RNA synthesis. Cell 41:259–267
    [Google Scholar]
  6. Bailey J. M., Davidson N. 1976; Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70:75–85
    [Google Scholar]
  7. Banerjee A. K. 1987; The transcription complex of vesicular stomatitis virus. Cell 48:363–364
    [Google Scholar]
  8. Bell J. C., Prevec L. 1985; Phosphorylation sites on phosphoprotein NS of vesicular stomatitis virus. Journal of Virology 54:697–702
    [Google Scholar]
  9. Bell J. C., Brown E. G., Takayesu D., Prevec L. 1984; Protein kinase activity associated with immunoprecipitates of the vesicular stomatitis virus phosphoprotein NS. Virology 132:229–238
    [Google Scholar]
  10. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  11. Blumberg B. M., Leppert M., Kolakofsky D. 1981; Interaction of VSV leader RNA and nucleocapsid protein may control VSV genome replication. Cell 23:837–845
    [Google Scholar]
  12. Blumberg B. M., Giorgi C., Kolakofsky D. 1983; N protein of vesicular stomatitis virus selectively encapsidates leader RNA in vitro. Cell 32:559–567
    [Google Scholar]
  13. Cave D. R., Hagen F. S., Palma E. L., Huang A. S. 1984; Detection of vesicular stomatitis virus RNA and its defective interfering particles in individual mouse brains. Journal of Virology 50:86–91
    [Google Scholar]
  14. Davis N. L., Wertz G. W. 1982; Synthesis of vesicular stomatitis virus negative-strand RNA in vitro: dependence on viral protein synthesis. Journal of Virology 41:821–832
    [Google Scholar]
  15. Davis N. L., Arnheiter H., Wertz G. W. 1986; Vesicular stomatitis virus N and NS proteins form multiple complexes. Journal of Virology 59:751–754
    [Google Scholar]
  16. De B. P., Banerjee A. K. 1985; Requirements and functions of vesicular stomatitis virus L and NS proteins in the transcription process in vitro. Biochemical and Biophysical Research Communications 126:40–49
    [Google Scholar]
  17. Emerson S. U. 1976; Vesicular stomatitis virus: structure and function of virion components. Current Topics in Microbiology and Immunology 73:1–34
    [Google Scholar]
  18. Emerson S. U., Yu Y-H. 1975; Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus. Journal of Virology 15:1348–1356
    [Google Scholar]
  19. Fiszman M., Leaute J.-B., Chany C., Girard M. 1974; Mode of action of acid pH values on the development of vesicular stomatitis virus. Journal of Virology 13:801–808
    [Google Scholar]
  20. Flamand A., Bishop D. H. L. 1974; In vivo synthesis of RNA by vesicular stomatitis virus and its mutants. Journal of Molecular Biology 87:31–53
    [Google Scholar]
  21. Hsu C-H., Kingsbury D. W. 1982; NS phosphoprotein of vesicular stomatitis virus: subspecies separated by electrophoresis and isoelectric focusing. Journal of Virology 42:342–345
    [Google Scholar]
  22. Hsu C-H., Morgan E. M., Kingsbury D. W. 1982; Site-specific phosphorylation regulates the transcriptive activity of vesicular stomatitis virus NS protein. Journal of Virology 43:104–112
    [Google Scholar]
  23. Hudson L. D., Condra C., Lazzarini R. A. 1986; Cloning and expression of aviral phosphoprotein: structure suggests vesicular stomatitis virus NS may function by mimicking an RNA template. Journal of General Virology 67:1571–1579
    [Google Scholar]
  24. Imblum R. L., Wagner R. R. 1974; Protein kinase and phosphoproteins of vesicular stomatitis virus. Journal of Virology 13:113–124
    [Google Scholar]
  25. Isaac C. L., Keene J. D. 1982; RNA polymerase-associated interactions near template promoter sequences of defective interfering particles of vesicular stomatitis virus. Journal of Virology 43:241–249
    [Google Scholar]
  26. Iverson L. E., Rose J. K. 1981; Localized attenuation and discontinuous synthesis during vesicular stomatitis virus transcription. Cell 23:477–484
    [Google Scholar]
  27. Kingsford L., Emerson S. U. 1980; Transcriptional activities of different phosphorylated species of NS protein purified from vesicular stomatitis virions and cytoplasm of infected cells. Journal of Virology 33:1097–1105
    [Google Scholar]
  28. Krieg P., Amtmann E., Sauer G. 1983; The simultaneous extraction of high-molecular-weight DNA and of RNA from solid tumors. Analytical Biochemistry 134:288–294
    [Google Scholar]
  29. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  30. Lesnaw J. A., Dickson L. R., Curry R. H. 1979; Proposed replicative role of the NS polypeptide of vesicular stomatitis virus: structural analysis of an electrophoretic variant. Journal of Virology 31:8–16
    [Google Scholar]
  31. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  32. Marcus P. I., Engelhardt D. L., Hunt I. M., Sekellick M. J. 1971; Interferon action: inhibition of vesicular stomatitis virus RNA synthesis induced by virion-bound polymerase. Science 174:593–598
    [Google Scholar]
  33. Marnell L. L., Summers D. F. 1984; Characterization of the phosphorylated small enzyme subunit NS of the vesicular stomatitis virus RNA polymerase. Journal of Biological Chemistry 259:13518–13524
    [Google Scholar]
  34. Melber K., Krieg P., Fürstenberger G., Marks F. 1986; Molecular cloning OF sequences activated during multi-stage carcinogenesis in mouse skin. Carcinogenesis 7:317–322
    [Google Scholar]
  35. Mellon M. G., Emerson S. U. 1978; Rebinding of transcriptase components (L and NS proteins) to the nucleocapsid template of vesicular stomatitis virus. Journal of Virology 27:560–567
    [Google Scholar]
  36. Möller A. 1967 In Ullmanns Enzyklopädien der Technischen Chemie 98 pp 718–728 Foerst W. Edited by Munich: Urban und Schwarzenberg;
    [Google Scholar]
  37. Patton J. T., Davis N. L., Wertz G. W. 1984; N protein alone satisfies the requirement for protein synthesis during RNA replication of vesicular stomatitis virus. Journal of Virology 49:303–309
    [Google Scholar]
  38. Rae B. P., Elliott R. M. 1986; Characterization of the mutations responsible for the electrophoretic mobility differences in the NS proteins of vesicular stomatitis virus New Jersey complementation group E mutants. Journal of General Virology 67:2635–2643
    [Google Scholar]
  39. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitroby nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  40. Sánchez A., De B. P., Banerjee A. K. 1985; In vitrophosphorylation of NS protein by the L protein of vesicular stomatitis virus. Journal of General Virology 66:1025–1036
    [Google Scholar]
  41. Sauer G., Amtmann E., Melber K., Knapp A., Müller K., Hummel K., Scherm A. 1984; DNA and RNA virus species are inhibited by xanthates, a class of antiviral compounds with unique properties. Proceedings of the National Academy of Sciences U.S.A.: 813263–3267
    [Google Scholar]
  42. Sokol F., Koprowski H. 1975; Structure-function relationships and mode of replication of animal rhabdoviruses. Proceedings of the National Academy of Sciences U.S.A.: 72:933–936
    [Google Scholar]
  43. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences U.S.A.: 775201–5205
    [Google Scholar]
  44. Wertz G. W., Levine M. 1973; RNA synthesis by vesicular stomatitis virus and a small plaque mutant: effects of cycloheximide. Journal of Virology 12:253–264
    [Google Scholar]
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