1887

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Volume 68, Issue 3

Characterization of a Molecular Clone of RFM/Un Mouse Chromosomal DNA that Contains a Full-length Endogenous Murine Leukaemia Virus-related Proviral Genome Free

Abstract

SUMMARY

A 12·4 kbp dIII chromosomal DNA fragment harbouring an apparently intact 9·2 kbp endogenous murine leukaemia virus (MuLV)-related proviral genome was isolated from an RFM/Un strain mouse by molecular cloning and designated pRFM # 6. Nucleotide sequence analysis revealed the following characteristic features in the pRFM # 6 provirus: a distinct 200 bp sequence in the long terminal repeat (LTR) mid-U3 region, a primer binding site for glutamine tRNA, a 3′ region encoding an ‘endonuclease’ protein of 390 amino acids, and the mink cell focusforming virus type-specific sequence at the 5′ portion of the gene. The 699 bp 5′ LTR and 700 bp 3′ LTR of pRFM # 6 provirus were identical except for three base changes in the U3 ‘enhancer’ region. At the cell-provirus DNA junction, 4 bp direct repeats were present. The proviral genome was found at the same chromosomal DNA site in BALB/c, AKR, C3H, CBA and RFM strain mice, but not in NFS/N or C57BL/6 strain mice.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome , murine leukaemia virus and provirus
© The Journal of General Virology 1987
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1987-03-01
2024-04-27
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Characterization of a Molecular Clone of RFM/Un Mouse Chromosomal DNA that Contains a Full-length Endogenous Murine Leukaemia Virus-related Proviral Genome
J. Gen. Virol. 68, 683 (1987); https://doi.org/10.1099/0022-1317-68-3-683
/content/journal/jgv/10.1099/0022-1317-68-3-683
/content/journal/jgv/10.1099/0022-1317-68-3-683
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