1887

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Volume 68, Issue 4

Generation of a Herpes Simplex Virus Type 1 Variant Devoid of I Sites Free

Abstract

SUMMARY

Using both selection enrichment and site-directed mutagenesis, a herpes simplex virus type 1 (HSV-1) strain 17 genome lacking all four I sites has been generated. The site at 0·45 map units which lies within the gene coding for a polypeptide of 28 000 molecular weight was removed by selection enrichment, while the site at 0·29 map units which lies within the gene coding for glycoprotein H was removed by site-directed mutagenesis. The parental virus from which these two I sites were deleted had previously had the sites at 0·07 and 0·63 map units removed through selection enrichment. The variant devoid of I sites (X4) showed normal growth characteristics; its phenotype was normal apart from the absence of the thymidine kinase protein, which is believed to be unrelated to the loss of I sites.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): HSV-1 , synthetic oligonucleotide and XbaI site deletion
© Society for General Microbiology 1987
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1987-04-01
2024-05-08
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Generation of a Herpes Simplex Virus Type 1 Variant Devoid of XbaI Sites
J. Gen. Virol. 68, 1165 (1987); https://doi.org/10.1099/0022-1317-68-4-1165
/content/journal/jgv/10.1099/0022-1317-68-4-1165
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