1887

Abstract

SUMMARY

We investigated the properties of amber mutants of coliphage T5 defective in a late stage of phage assembly. The non-functional heads synthesized by mutants 25 or aw 158 were unable to combine with functional tails to produce viable phage particles. These mutants were shown to lack a single protein, designated the head-tail linker protein (HTLp), which was identified by polyacrylamide gel electrophoresis and Western blot analysis and had an of 18000. An extract containing the HTLp, when added to the HTLp-deficient heads, restored their ability to combine with functional tails.

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/content/journal/jgv/10.1099/0022-1317-68-4-957
1987-04-01
2024-04-27
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