1887

Abstract

SUMMARY

Aphid transmission experiments with the transmissible isolate Cabbage B and the non-transmissible isolate Campbell of cauliflower mosaic virus (CaMV), using different combinations of purified viruses, viroplasms and cellular fractions, revealed that helper component (HC) activity for CaMV transmissibility was associated only with the viroplasm preparations of the transmissible isolate. In electrophoretic analyses of purified virions, viroplasms and cellular fractions from plants infected with both CaMV isolates as has already been described, a PI 8 polypeptide in the purified viroplasm preparations from the transmissible CaMV isolate was the only difference between the two isolates. These results suggest that the purified viroplasms of transmissible CaMV isolates contain the HC in a functional form, and that the P18 polypeptide is the active HC for CaMV aphid transmissibility or a part of it.

Keyword(s): CaMV , helper activity and viroplasms
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-8-2063
1987-08-01
2024-05-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/8/JV0680082063.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-8-2063&mimeType=html&fmt=ahah

References

  1. Al Ani R., Pfeiffer P., Whitechurch O., Lesot A., Lebeurier G., Hirth L. 1980; A virus-specified protein produced upon infection by cauliflower mosaic virus (CaMV). Amales de Virologie 131E:33–53
    [Google Scholar]
  2. Armour S. L., Melcher U., Pirone T. P., Lyttle D. J., Essemberg R. C. 1983; Helper component for aphid transmission encoded by region II of cauliflower mosaic virus DNA. Virology 129:25–30
    [Google Scholar]
  3. Covey S. N. 1985; Organization and expression of the cauliflower mosaic virus genome. In Molecular Plant Virology 2 pp 121–190 Davies J. W. Edited by Florida: CRC Press;
    [Google Scholar]
  4. Givord L., Xiong C., Giband M., Koenig I., Hohn T., Lebeurier G., Hirth L. 1984; A second cauliflower mosaic virus gene product influences the structure of the viral inclusion body. EMBO Journal 3:1423–1427
    [Google Scholar]
  5. Hull R., Shepherd R. J., Harvey J. D. 1976; Cauliflower mosaic virus: an improved purification procedure and some properties of the virus particles. Journal of General Virology 31:93–100
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  7. Lung M. C. Y., Pirone T. P. 1972; Datura stramonium a local lesion host for certain isolates of cauliflower mosaic virus. Phytopathology 62:1473–1474
    [Google Scholar]
  8. Lung M. C. Y., Pirone T. P. 1973; Studies on the reason for differential transmissibility of cauliflower mosaic virus isolates by aphids. Phytopathology 63:910–914
    [Google Scholar]
  9. Lung M. C. y., Pirone T. P. 1974; Acquisition factor required for aphid transmission of purified cauliflower mosaic virus. Virology 60:260–264
    [Google Scholar]
  10. Markham P. G., Hull R. 1985; Cauliflower mosaic virus aphid transmission facilitated by transmission factors from other caulimoviruses. Journal of General Virology 66:921–923
    [Google Scholar]
  11. Modjtahedi N., Volovitch M., Mazzolini L., Yot P. 1985; Comparison of the predicted secondary structure of aphid transmission factor for transmissible and non-transmissible cauliflower mosaic virus strains. FEBS Letters 181:223–228
    [Google Scholar]
  12. Pirone T. P., Thornbury D. W. 1984; The involvement of a helper component in non persistent transmission of plant viruses by aphids. Microbiological Sciences 1:191–193
    [Google Scholar]
  13. Rubio-Huertos M. 1950; Estudio sobre inclusiones producidas por virus en plantas. Microbiología Española 3:207–231
    [Google Scholar]
  14. Shepherd R. J., Richins R., Shalla T. A. 1980; Isolation and properties of the inclusion bodies of cauliflower mosaic virus. Virology 102:389–400
    [Google Scholar]
  15. Shockey M. W., Gardner C. O. Jr Melcher U., Essenberg R. C. 1980; Polypeptides associated with inclusion bodies from leaves of turnip infected with cauliflower mosaic virus. Virology 105:575–581
    [Google Scholar]
  16. Woolston C. J., Covey S. N., Penswick J. R. 1983; Aphid transmission and a polypeptide are specified by a defined region of the cauliflower mosaic virus genome. Gene 23:15–23
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-8-2063
Loading
/content/journal/jgv/10.1099/0022-1317-68-8-2063
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error