1887

Abstract

Summary

Herpes simplex virus type 1 (HSV-1) strains F, HF and HFEM were studied with respect to pathogenicity in mice and growth characteristics and compared to the neurovirulent HSV-1 strains 17 syn and KOS. All three viruses demonstrated reduced virulence in mouse brains and were completely avirulent after footpad inoculation. They were shown to express high levels of thymidine kinase activity. Investigations concerning the virulence phenotype indicated that the defect(s) in strains F, HF and HFEM related to general replication deficiency in mouse cells. It was also shown that although the replication restriction observed for strains F and HF was specific for murine cells, strain HFEM did not replicate well in any cell type tested. Additional studies indicated that the avirulence phenotype which followed peripheral inoculation was related to different genotypes, since strain F complemented HF and HFEM and, as expected, the latter two agents did not complement each other. All three agents were found to complement the non-neuroinvasive strain KOS. Finally, the data also show that two herpesviruses which are highly restricted in murine cells (e.g. strains F and HF) can still interact in the animal and produce a lethal infection.

Keyword(s): HSV-1 , TK and virulence
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/content/journal/jgv/10.1099/0022-1317-68-9-2389
1987-09-01
2024-04-26
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References

  1. Becker Y., Hadar J., Tabor E., Ben-Hur T., Raibstein I., Rosen A., Darai G. 1986; A sequence in Hpdl-Pfragment of herpes simplex virus-1 DNA determines intraperitoneal virulence in mice. Virology 149:255–259
    [Google Scholar]
  2. Burnet F. M., Lush D. 1939; Studies on experimental herpes infection in mice, using the chorioallantoic technique. Journal of Pathology 39:241–259
    [Google Scholar]
  3. Centifanto-Fitzgerald Y. M., Yamaguchi T., Kaufman H. E., Tognon M., Roizman B. 1982; Ocular disease pattern induced by herpes simplex virus is genetically determined by a specific region of viral DNA. Journal of Experimental Medicine 155:475–489
    [Google Scholar]
  4. Cook M. L., Stevens J. G. 1973; Pathogenesis of herpetic neuritis and ganglionitis in mice: evidence for intraaxonal transport of infection. Infection and Immunity 7:272–288
    [Google Scholar]
  5. Dix R. D., Mckendall R. R., Baringer J. R. 1983; Comparative neurovirulence of herpes simplex virus type 1 strains after peripheral or intracerebral inoculation of BALB/c mice. Infection and Immunity 40:103–112
    [Google Scholar]
  6. Ejercito P. M., Kieff E. D., Roizman B. 1968; Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. Journal of General Virology 2:357–364
    [Google Scholar]
  7. Flexner S., Amoss H. L. 1925; Contributions to the pathology of experimental viral encephalitis. Journal of Experimental Medicine 41:215
    [Google Scholar]
  8. Jamieson A. T., Subak-Sharpe J. H. 1974; Biochemical studies on herpes simplex virus-specified deoxypyri- midine kinase activity. Journal of General Virology 24:481–492
    [Google Scholar]
  9. Javier J. T., Sedarati F., Stevens J. G. 1986; Two avirulent herpes simplex viruses interact in the animal to generate virulent recombinants and a lethal infection. Science 234:746–748
    [Google Scholar]
  10. Kit S., Piekarski L. J., Dubbs D. R. 1963; Induction of thymidine kinase by vaccinia-infected mouse fibroblasts. Journal of Molecular Biology 6:22–33
    [Google Scholar]
  11. Merchant D. J., Kahn R. H., Murphy W. H. 1969 Handbook of Cell and Organ Culture, 2nd edn.. Minneapolis: Burgess;
    [Google Scholar]
  12. Olitsky P. K., Schlesinger R. W. 1941; Effect of local edema and inflammation in the skin of the mouse on the progression of herpes virus. Science 93:574–575
    [Google Scholar]
  13. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  14. Rosen A., Gelderblom H., Darai G. 1985; Transduction of virulence in herpes simplex type 1 from A pathogenic to an apathogenic strain by a cloned viral DNA fragment. Medical Microbiology and Immunology 173:257–278
    [Google Scholar]
  15. Thompson R. L., Stevens J. G. 1983; Biological characterization of a herpes simplex virus intertypic recombinant which is completely and specifically non-neurovirulent. Virology 131:171–179
    [Google Scholar]
  16. Thompson R. L., Cook M. L., Devi-Rao G. B., Wagner E. K., Stevens J. G. 1986; Functional and molecular analyses of the avirulent wild-type herpes simplex virus type 1 strain KOS. Journal of Virology 58:203–211
    [Google Scholar]
  17. Watson D. H., Shedden W. I. H., Elliot A., Tetsuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11:339–408
    [Google Scholar]
  18. Watson K., Stevens J. G., Cook M. L., Subak-Sharpe J. H. 1980; Latency competence of thirteen HSV-1 temperature-sensitive mutants. Journal of General Virology 49:149–159
    [Google Scholar]
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