1887

Abstract

Summary

Cotransfection of nuclear polyhedrosis virus (RoMNPV) and HI or I fragments from the genome of nuclear polyhedrosis virus (AcMNPV) yielded a high frequency of virus recombinants in larvae. When larvae were cotransfected either with the entire genome of AcMNPV and RoMNPV restriction fragments or with a mixture of the two viruses, only AcMNPV was produced. In the virus recombinants, the prevalence of certain regions from the genome of the donor virus (AcMNPV) varied considerably, e.g. from 0% in the 35 to 41 map unit region area to a high level in the 20 to 23 map unit region. The AcMNPV polyhedrin gene was shown to be inherited preferentially.

Keyword(s): AcMNPV , recombination and RoMNPV
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/content/journal/jgv/10.1099/0022-1317-69-1-177
1988-01-01
2024-04-30
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References

  1. COCHRAN M. A., CARSTENS E. B., EATON B. T., FAULKNER P. 1982; Molecular cloning and physical mapping of restriction endonuclease fragments of Autographa californica nuclear polyhedrosis virus DNA. Journal of Virology 41:940–946
    [Google Scholar]
  2. CRAWFORD A. M., FAULKNER P., KALMAKOFF J. 1978; Comparison of solid-phase radioimmuno-assays for baculoviruses. Applied and Environmental Microbiology 36:18–24
    [Google Scholar]
  3. CROIZIER G., QUIOT J. M. 1981; Obtention and analysis of two genetic recombinants of baculoviruses of Lepidoptera, Autographa californica Speyer and Galleria mellonella L. Annales de Virologie 132:3–18
    [Google Scholar]
  4. CROIZIER G., GODSE D., VLAK J. 1980; Selection de types viraux dans les infections doubles à baculovirus chez les larves de lépidoptères. Comptes rendus hebdomadaires des séances de l’Académie des sciences, série D 290:579–582
    [Google Scholar]
  5. GORDON J. D., CARSTENS E. 1984; Phenotypic characterization of physical mapping of a temperature-sensitive mutant of Autographa californica nuclear polyhedrosis virus defective in DNA synthesis. Virology 138:69–81
    [Google Scholar]
  6. JEWELL J. E., MILLER L. K. 1980; DNA sequence homology relationships among six lepidopteran nuclear polyhedrosis viruses. Journal of General Virology 48:161–175
    [Google Scholar]
  7. KELLY D. C., WANG X. 1981; The infectivity of nuclear polyhedrosis virus DNA. Annales de Virologie 132:247–259
    [Google Scholar]
  8. KNIPE D. M., RUYECHAN W. J., ROIZMAN B. 1979; Molecular genetics of herpes simplex virus. III. Fine mapping of a genetic locus determining resistance to phosphonoacetate by two methods of marker transfer. Journal of Virology 29:698
    [Google Scholar]
  9. LEDERBERG E. M., COHEN S. N. 1974; Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. Journal of Bacteriology 119:1072–1074
    [Google Scholar]
  10. MANLVTIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. MASKOS C. B., MILTENBURGER H. G. 1981; SDS-PAGE comparative studies on the polyhedral and viral polypeptides of the nuclear polyhedrosis viruses of Mamestra brassicae, Autographa californica and Lymantria dispar. Journal of Invertebrate Pathology 37:174–180
    [Google Scholar]
  12. REED L. J., MUENCH H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  13. SKURATOVSKAYA I. N., STOKOVSKAYA L. I., ZHEREBTSOVA E.M., GUDZ-GORBAN A. P. 1977; Supercoiled DNA of NPV of Galleria mellonella L. Archives of Virology 53:79–86
    [Google Scholar]
  14. SMITH G. E., SUMMERS M. D. 1980; Restriction map of Rachiplusia ou and Rachiplusia ou-Autographa californica baculovirus recombinants. Journal of Virology 33:311–319
    [Google Scholar]
  15. SMITH G. E., SUMMERS M. D. 1981; Application of a novel radioimmunoassay to identify baculovirus structural proteins that share interspecies antigenic determinants. Journal of Virology 39:125–135
    [Google Scholar]
  16. SMITH G. E., FRASER M. J., SUMMERS M. D. 1983; Molecular engineering of Autographa californica nuclear polyhedrosis virus genome: deletion mutations within the polyhedrin gene. Journal of Virology 46:583–584
    [Google Scholar]
  17. SUMMERS M. D., SMITH G. E., KNELL J. D., BURAND J. P. 1980; Physical maps of Autographa californica and Rachiplusia ou nuclear polyhedrosis virus recombinants. Journal of Virology 34:693–703
    [Google Scholar]
  18. VIEIRA J., MESSING J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  19. VLAK J. M., SMITH G. E. 1982; Orientation of the genome of Autographa californica nuclear polyhedrosis virus: a proposal. Journal of Virology 41:1118–1121
    [Google Scholar]
  20. VLAK J. M., SMITH G. E., SUMMERS M. D. 1981; Hybridization selection and in vitro translation of Autographa californica nuclear polyhedrosis virus mRNA. Journal of Virology 40:762–771
    [Google Scholar]
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