1887

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Volume 69, Issue 3

The Principal Hydrogen Donor for the Herpes Simplex Virus Type 1-encoded Ribonucleotide Reductase in Infected Cells Is a Cellular Thioredoxin Free

Abstract

Summary

In this study herpes simplex virus type 1-encoded ribonucleotide reductase was shown to be able to utilize thioredoxin purified from the cyanobacterium as a hydrogen donor for the enzyme. An assay has been developed to search for proteins which can function as a hydrogen donor for the viral ribonucleotide reductase. A protein has been identified and purified to homogeneity from infected cell extracts by a combination of fast protein liquid chromatography and gel filtration. This protein, which is also present in mock-infected cells, has been identified as a host cell thioredoxin by similarities in its physical characteristics with other thioredoxins. No evidence for the existence of a major virus-induced thioredoxin was obtained, suggesting that the host cell thioredoxin functions as the hydrogen donor for the herpes simplex virus type 1 ribonucleotide reductase in the infected cell.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): HSV-1 , ribonucleotide reductase and thioredoxin
© Society for General Microbiology 1988
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1988-03-01
2024-05-05
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The Principal Hydrogen Donor for the Herpes Simplex Virus Type 1-encoded Ribonucleotide Reductase in Infected Cells Is a Cellular Thioredoxin
J. Gen. Virol. 69, 515 (1988); https://doi.org/10.1099/0022-1317-69-3-515
/content/journal/jgv/10.1099/0022-1317-69-3-515
/content/journal/jgv/10.1099/0022-1317-69-3-515
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