1887

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Volume 69, Issue 6

Interaction of Polylysine with the Cellular Receptor for Herpes Simplex Virus Type 1 Free

Abstract

Summary

We earlier reported that neomycin blocked reversibly the binding of herpes simplex virus type 1 (HSV-1) to the receptor of BHK cells, while the binding of HSV-2 to the receptor was unaffected. We could not determine whether the effect was on the virus particle, the receptor, or both. We have now tested several other cationic substances, and report that polylysine (and polyarginine) block the binding of HSV-1 to the receptor by interfering with the cellular receptor function; higher molecular weight polylysines were more potent than those of lower molecular weight. Polylysine and neomycin showed additive effects. , polylysine showed the same strong binding to the plasma membrane phosphoinositides as did neomycin. Together these data suggest that the drugs may have a common target in the cell membrane. The HSV-1 and HSV-2 virus particles were unaffected by the drugs, as was the cellular HSV-2 receptor.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cellular , HSV-1 , polylysine and receptor
© Society for General Microbiology 1988
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1988-06-01
2024-05-02
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References

  1. ADDISON C, RIXON F. J., PALFREYMAN J. W., O’HARA M., PRESTON V. G. 1984; Characterisation of a herpes simplex virus type 1 mutant which has a temperature-sensitive defect in penetration of cells and assembly of capsids. Virology 138:246–259
     [Google Scholar]
  2. BASHFORD C. L., ALDER G. M., MENESTRINA G., MICKLEM K. J., MURPHY J. J., PASTERNAK C. A. 1986; Membrane damage by hemolytic viruses, toxins, complement, and other cytotoxic agents. Journal of Biological Chemistry 261:9300–9308
     [Google Scholar]
  3. BLIGH E. G., DYER W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
     [Google Scholar]
  4. BROWN S. M., RITCHIE D. A., SUBAK-SHARPE J. H. 1973; Genetic studies with herpes simplex virus type 1. The isolation of temperature-sensitive mutants, their arrangement into complementation groups and recombination analysis leading to a linkage map. Journal of General Virology 18:329–346
     [Google Scholar]
  5. CARNEY D. H., SCOTT D. L., GORDON E. A., LABELLE E. F. 1985; Phosphoinositides in mitogenesis: neomycin inhibits thrombin-stimulated phosphoinositide turnover and initiation of cell proliferation. Cell 42:479–488
     [Google Scholar]
  6. COCKCROFT S., GOMPERTS B. D. 1985; Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase. Nature, London 314:534–536
     [Google Scholar]
  7. CRABB J. H., JACKSON R. C. 1986; Polycation inhibition of exocytosis from sea urchin egg cortex. Journal of Membrane Biology 91:85–96
     [Google Scholar]
  8. DE CHAFFOY DE COURCELLES D., ROEVENS P., VAN BELLE H. 1984; 12-O-Tetradecanoylphorbol 13-acetate stimulates inositol lipid phosphorylation in intact human platelets. FEBS Letters 173:389–393
     [Google Scholar]
  9. DOWNES C. P., MICHELL R. H. 1981; The polyphosphoinositide phosphodiesterase of erythrocyte membranes. Biochemical Journal 198:133–140
     [Google Scholar]
  10. GAD A. E., BENTAL M., ELYASHIV G., WEINBERG H. 1985; Promotion and inhibition of vesicle fusion by polylysine. Biochemistry 24:6277–6282
     [Google Scholar]
  11. LANGELAND N., HAARR L., HOLMSEN H. 1986; Polyphosphoinositide metabolism in baby-hamster kidney cells infected with herpes simplex virus type 1. Biochemical Journal 237:707–712
     [Google Scholar]
  12. LANGELAND N., HAARR L., HOLMSEN H. 1986; Evidence that neomycin inhibits HSV 1 infection of BHK cells. Biochemical and Biophysical Research Communications 141:198–203
     [Google Scholar]
  13. LANGELAND N., HOLMSEN H., LILLEHAUG J. R., HAARR L. 1987; Evidence that neomycin inhibits binding of herpes simplex virus type 1 to the cellular receptor. Journal of Virology 61:3388–3393
     [Google Scholar]
  14. LEIDERMAN L. J., CRISS W. E., OKA T. 1985; The characterization and regulation of a polyamine-responsive, cyclic nucleotide-independent protein kinase activity in the mouse mammary gland. Biochimica et biophysica acta 844:95–104
     [Google Scholar]
  15. LE PETIT J., NOBILI O., BOYER J. 1986; Stimulation of brain lipase activity by polyamines. Comparison with the effect of ACTH. Pharmacology, Biochemistry and Behaviour 24:1543–1545
     [Google Scholar]
  16. LIN S.-Y. S., LIAO C, LEE C Y. 1977; Mechanism of anticholinesterase activities of cardiotoxin, protamine and polylysine. Biochemical Journal 161:229–232
     [Google Scholar]
  17. LOW M. G. 1987; Biochemistry of the glycosyl-phosphatidylinositol membrane protein anchors. Biochemical Journal 244:1–13
     [Google Scholar]
  18. MACPHERSON I., STOKER M. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
     [Google Scholar]
  19. MARSDEN H. S., CAMPBELL M. E. M., HAARR L., FRAME M. C, PARRIS D. S., MURPHY M., HOPE R. G., MULLER M. T., PRESTON C. M. 1987; The 65000-Mr DNA-binding and virion rrans-inducing proteins of herpes simplex virus type 1. Journal of Virology 61:2428–2437
     [Google Scholar]
  20. MOHANTY J. G., ROSENTHAL K. S. 1986; 2-Deoxy-d-glucose inhibition of herpes simplex virus type-1 receptor expression. Antiviral Research 6:137–149
     [Google Scholar]
  21. PALMER F. B. ST. C. 1981; Chromatography of acidic phospholipids on immobilized neomycin. Journal of Lipid Research 22:1296–1300
     [Google Scholar]
  22. PRAKASH J., FOXE M. J. 1985; Optimisation of conditions for infection of isolated potato protoplasts with potato virus X. Archives of Virology 85:269–279
     [Google Scholar]
  23. REID D. G., GAJJAR K. 1987; A proton and carbon 13 nuclear magnetic resonance study of neomycin B and its interaction with phosphatidylinositol 4,5-bisphosphate. Journal of Biological Chemistry 262:7967–7972
     [Google Scholar]
  24. SASTRASINH M., KNAUSS T. C, WEINBERG J. M., HUMES H. D. 1982; Identification of the aminoglycoside binding site in rat renal brush border membranes. Journal of Pharmacology and Experimental Therapeutics 222:350–358
     [Google Scholar]
  25. SCHACHT J. 1978; Purification of polyphosphoinositides by chromatography on immobilized neomycin. Journal of Lipid Research 19:1063–1067
     [Google Scholar]
  26. SCHACHT J. 1979; Isolation of an aminoglycoside receptor from guinea pig inner ear tissues and kidney. Archives of Oto-rhino-laryngology 224:129–134
     [Google Scholar]
  27. SHEN W.-C., RYSER H. J.-P. 1979; Poly(L-lysine) and poly(d-lysine) conjugates of methotrexate: different inhibitory effects on drug resistant cells. Molecular Pharmacology 16:614–622
     [Google Scholar]
  28. SHEN W.-C., RYSER H. J.-P., LAMANNA L. 1985; Disulfide spacer between methotrexate and poly(d-lysine). Journal of Biological Chemistry 260:10905–10908
     [Google Scholar]
  29. STAHMANN M. A., GRAF L. H., PATTERSON E. L., WALKER J. C, WATSON D. W. 1951; The inhibition of tobacco mosaic virus by synthetic lysine polypeptides. Journal of Biological Chemistry 189:45–52
     [Google Scholar]
  30. TACHIBANA M., MORIOKA H., MACHINO M., AMAGAI T., MIZUKOSHI O. 1986; Aminoglycoside binding sites in the cochlea as revealed by neomycin-gold labelling. Histochemistry 85:301–304
     [Google Scholar]
  31. TIMBURY M. C. 1971; Temperature-sensitive mutants of herpes simplex virus type 2. Journal of General Virology 13:373–376
     [Google Scholar]
  32. USTER P. S., DEAMER D. W. 1985; pH-dependent fusion of liposomes using titratable polycations. Biochemistry 24:1–8
     [Google Scholar]
  33. VAHLNE A., SVENNERHOLM B., LYCKE E. 1979; Evidence for herpes simplex virus type-selective receptors on cellular plasma membranes. Journal of General Virology 44:217–225
     [Google Scholar]
  34. WALTER A., STEER C. J., BLUMENTHAL R. 1986; Polylysine induces pH-dependent fusion of acidic phospholipid vesicles: a model for polycation-induced fusion. Biochimica et biophysica acta 861:319–330
     [Google Scholar]
  35. WHITAKER M., AITCHISON M. 1985; Calcium-dependent polyphosphoinositide hydrolysis is associated with exocytosis in vitro. FEBS Letters 182:119–124
     [Google Scholar]
  36. WILLIAMS P. D., HOTTENDORF G. H., BENNETT D. B. 1986; Inhibition of renal membrane binding and nephrotoxicity of aminoglycosides. Journal of Pharmacology and Experimental Therapeutics 237:919–925
     [Google Scholar]
  37. ZEIGLER R. J., POZOS R. S. 1981; Effects of lectins on peripheral infection by herpes simplex virus of rat sensory neurons in culture. Infection and Immunity 34:588–595
     [Google Scholar]
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Interaction of Polylysine with the Cellular Receptor for Herpes Simplex Virus Type 1
J. Gen. Virol. 69, 1137 (1988); https://doi.org/10.1099/0022-1317-69-6-1137
/content/journal/jgv/10.1099/0022-1317-69-6-1137
/content/journal/jgv/10.1099/0022-1317-69-6-1137
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