1887

Abstract

Summary

The effect of interferon (IFN) treatment on the early stages of herpes simplex virus type 1 (HSV-1) replication in three types of human cells was investigated. Interferon pretreatment was shown to reduce the steady state levels of both total and polysome-bound HSV-1 immediate early α mRNAs. Using the nuclear run-off transcription assay, we showed that IFN selectively inhibited transcription of the HSV-1 genes, with no effect on transcription of total cellular RNA or that of the β-tubulin RNA. Thus, IFN appears to inhibit the initiation of HSV-1 α gene transcription rather than affect the stability of the respective mRNAs. IFN did not prevent the HSV-1-induced early shut-off of host cellular protein synthesis caused by a structural protein of the infecting virus. This observation indicated that the IFN-mediated inhibition of HSV-1 replication is at a stage beyond viral penetration into the cytoplasm. These results suggested that IFN blocked HSV-1 replication primarily at a very early stage, during the onset of α mRNA transcription.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-6-1167
1988-06-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/6/JV0690061167.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-6-1167&mimeType=html&fmt=ahah

References

  1. BELKOWSKI L. S., SEN G. C. 1987; Inhibition of vesicular stomatitis viral mRNA synthesis by interferons. Journal of Virology 61:653–660
    [Google Scholar]
  2. BRENNAN M. B., STARK G. R. 1983; Interferon pretreatment inhibits simian virus 40 infections by blocking the onset of early transcription. Cell 33:811–816
    [Google Scholar]
  3. CAMPBELL M. E. M., PALFREYMAN J. W., PRESTON C. M. 1984; Identification of herpes simplex viras DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcription. Journal of Molecular Biology 180:1–19
    [Google Scholar]
  4. CATHALA G., SAVOURET J. F., MENDEZ B., WEST B. L., KARIN M., MARTIAL J. M., BAXTER J. D. 1983; Laboratory methods. A method for isolation of intact, translationally active ribonucleic acid. DNA 2:329–335
    [Google Scholar]
  5. CAYLEY P. J., DAVIES J. A., MCCULLAUGH K. G., KERR I. M. 1984; Activation of the ppp(A2′p)nA system in interferon treated herpes simplex virus infected cells and evidence for novel inhibitors of the ppp(A2′p)nA dependent RNase. European Journal of Biochemistry 143:165–174
    [Google Scholar]
  6. CHATTERJEE S., LAKEMAN A. D., WHITNEY R. J., HUNTER E. 1984; Effect of cloned human interferons on the replication of and cell fusion induced by herpes simplex virus. Virus Research 1:81–87
    [Google Scholar]
  7. CHATTERJEE S., HUNTER E., WHITLEY R. 1985; Effect of cloned human interferons on protein synthesis and morphogenesis of herpes simplex virus. Journal of Virology 56:419–425
    [Google Scholar]
  8. DOMKE I., STRAUB P., JACOBSEN H., KIRCHNER H., PANET A. 1985; Inhibition of replication of herpes simplex virus in mouse macrophages by interferons. Journal of General Virology 66:2231–2236
    [Google Scholar]
  9. DOMKE I., STRAUB P., KIRCHNER H. 1986; Effect of interferon on replication of herpes simplex virus type 1 and 2 in human macrophages. Journal of Virology 60:37–42
    [Google Scholar]
  10. GLOGER I., PANET A. 1984; Synthesis of herpes simplex virus proteins in interferon-treated human cells. Journal of General Virology 65:1107–1111
    [Google Scholar]
  11. GODOWSKI P. I., KNIPE D. 1986; Transcriptional control of herpesvirus gene expression: gene functions required for positive and negative regulation. Proceedings of the National Academy of Sciences, U.S.A 83:256–260
    [Google Scholar]
  12. GREENBERG M. E., ZIFF E. B. 1984; Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature, London 311:433–437
    [Google Scholar]
  13. HARRIS-HAMILTON E., BACHENHEIMER S. L. 1985; Accumulation of herpes simplex virus type 1 RNAs of different kinetic classes in the cytoplasm of infected cells. Journal of Virology 53:144–151
    [Google Scholar]
  14. HONESS R. W., ROIZMAN B. 1975; Regulation of herpesvirus macromolecular synthesis: sequential transition of polypeptide synthesis requires functional viral polypeptides. Proceedings of the National Academy of Sciences, U.S.A 72:1276–1280
    [Google Scholar]
  15. HONIGMAN A., PANET A. 1983; A unique sequence in murine leukemia virus long terminal repeat functions as a termination signal for transcription in Escherichia coli. Journal of Virology 45:456–461
    [Google Scholar]
  16. JEN G., DETJEN B. M., THACH R. E. 1980; Shutoff of HeLa cell protein synthesis by encephalomyocarditis virus and poliovirus: a comparative study. Journal of Virology 35:150–156
    [Google Scholar]
  17. LENGYEL P. 1982; Biochemistry of interferons and their actions. Annual Review of Biochemistry 51:251–282
    [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  19. MACKEM S., ROIZMAN B. 1980; Regulation of herpesvirus macromolecular synthesis: transcription initiation sites and domains of a genes. Proceedings of the National Academy of Sciences, U.S.A 77:7122–7126
    [Google Scholar]
  20. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. MARZLUFF W. F., HUANG R. C C. 1984 Transcription of RNA in isolated nuclei. Transcription and Translation—A Practical Approach, 2nd edn.89–129 Edited by Hames B. D., Higgins S. J. Oxford & Washington, D.C.: IRL Press;
    [Google Scholar]
  22. MUNOZ A., CARRASCO L. 1984; Formation of non-infective herpesvirus particles in cultured cells treated with human interferon. Journal of General Virology 65:1069–1078
    [Google Scholar]
  23. O’HARE P., HAYWARD G. S. 1984; Expression of recombinant genes containing herpes simplex virus delayed-early and immediate-early regulatory regions and transactivation by herpes virus infection. Journal of Virology 52:522–531
    [Google Scholar]
  24. PALMITER R. D. 1974; Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undegraded polysomes and messenger ribonucleic acid. Biochemistry 13:3606–3614
    [Google Scholar]
  25. PANET A. 1983; Regulation of the antiviral and anticellular activities of interferon by exogenous double-stranded RNA. Molecular and Cellular Biochemistry 52:153–160
    [Google Scholar]
  26. PANET A., FALK H. 1983; Inhibition by interferon of herpes simplex virus thymidine kinase and DNA polymerase in infected and biochemically transformed cells. Journal of General Virology 64:1999–2006
    [Google Scholar]
  27. PURIFOY D. J. M., BENYESH-MELNICK M. 1975; DNA polymerase induction by DNA negative temperature sensitive mutants of herpes simplex virus type-2. Virology 68:374–386
    [Google Scholar]
  28. QUINLAN M. P., KNIPE D. M. 1985; Stimulation of expression of a herpes simplex virus DNA-binding protein by two viral functions. Molecular and Cellular Biology 5:957–963
    [Google Scholar]
  29. READ G. S., FRENKEL N. 1983; Herpes simplex virus mutants defective in the virion associated shut-off of host polypeptide synthesis and exhibiting abnormal synthesis of (immediate-early) viral polypeptides. Journal of Virology 46:498–512
    [Google Scholar]
  30. RIGBY P. W. J., DIECKMANN M., RHODES C, BERG P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  31. SCHEK N., BACHENHEIMER S. L. 1985; Degradation of cellular mRNAs induced by a virion-associated factor during herpes simplex virus infection of Vero cells. Journal of Virology 55:601–610
    [Google Scholar]
  32. SPEAR P. G., ROIZMAN B. 1980 Herpes simplex viruses. DNA Tumor Viruses615–745 Edited by Tooze J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  33. STRAUB P., DOMKE I., KIRCHNER H., JACOBSEN H., PANET A. 1986; Synthesis of herpes simplex virus proteins and nucleic acids in interferon-treated macrophages. Virology 150:411–418
    [Google Scholar]
  34. WEINHEIMER S. P., MCKNIGHT S. L. 1987; Transcriptional and post-transcriptional controls establish the cascade of herpes simplex virus protein synthesis. Journal of Molecular Biology 195:819–833
    [Google Scholar]
  35. WIGLER M., SILVERSTELN S., LEE L. S., PELLICER A., CHENG Y. C., AXEL R. 1977; Transfer of purified herpes virus thymidine kinase gene into mouse cells. Cell 11:223–232
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-6-1167
Loading
/content/journal/jgv/10.1099/0022-1317-69-6-1167
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error