1887

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Volume 69, Issue 6

The Response of Infants with Bronchiolitis to the Proteins of Respiratory Syncytial Virus Free

Abstract

Summary

Acute phase sera were collected from 28 infants hospitalized with bronchiolitis due to respiratory syncytial (RS) virus and convalescent sera were collected from 24 of them. The sera were assayed for neutralizing antibodies by plaque inhibition, for antibodies to the viral proteins by Western blot against partially purified RS virus, and for their ability to inhibit attachment and fusion. Among the 28 acute phase sera, 27 had antibody to the attachment glycoprotein (G), 16 had antibody to the fusion glycoprotein (F), but none had antibody to the matrix protein (VPM). Both the geometric mean anti-G titre, and the geometric mean anti-F titre correlated with the 50% neutralizing dose (ND) titre in the acute phase serum. Among the 24 convalescent sera, only four exhibited an increase in neutralizing antibody titre. The response to G appeared to be related to the acute phase ND titre. Of 17 infants with acute phase titres of less than 100 ND/ml, 10 responded to G while there was no response to this protein in seven infants with acute phase titres greater than 100 ND/ml. While only one infant responded to F, 18 responded to the phosphorylated nucleocapsid protein, VP32, and none responded to VPM. The ability of the acute phase sera to inhibit virus attachment to HeLa cells and to inhibit fusion correlated with the anti-G titre and the anti-F titre, respectively. However, there was no correlation between the inhibition of fusion and the anti-F titre in the convalescent sera, almost all of which inhibited fusion. These results suggest that the infected infants were responding to RS virus, but that their response to the viral proteins was either masked or slowed by residual maternal antibody. The inability to detect VPM in the acute and convalescent phase sera, as well as in 20 paired maternal and cord sera at a 1:50 dilution suggested that VPM, although it is one of the most prevalent viral proteins in both the virion and the infected cell, may be poorly antigenic in humans.

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Keyword(s): antibodies , bronchiolitis and respiratory syncytial virus
© Society for General Microbiology 1988
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1988-06-01
2024-05-02
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References

  1. BANGHAM C. R. M., OPENSHAW P. J. M., BALL L. A., KING A. M. Q., WERTZ G. W., ASKONAS B. A. 1986; Human and murine cytotoxic T cells specific to respiratory syncytial virus recognize the viral nucleoprotein (N), but not the major glycoprotein (G), expressed by vaccinia virus recombinants. Journal of Immunology 137:3973–3977
     [Google Scholar]
  2. BRUHN F. W., YEAGER A. S. 1977; Respiratory syncytial virus in early infancy. American Journal of Diseases of Children 131:145–148
     [Google Scholar]
  3. CHANOCK R. M., PARROTT R. H. 1965; Acute respiratory disease in infancy and childhood: present understanding and prospects for prevention. Pediatrics 36:21–39
     [Google Scholar]
  4. FERME B. F., GERIN J. L. 1982; Immunochemical identification of viral and non viral proteins of the respiratory syncytial virion. Infection and Immunity 37:243–249
     [Google Scholar]
  5. FERME B. F., DAPOLITO G., COTE P. J. JR, GERIN J. L. 1985; Kinetics of synthesis of respiratory syncytial virus glycoproteins. Journal of General Virology 66:1983–1990
     [Google Scholar]
  6. GARDNER P. S. 1968; Virus infections and respiratory disease of childhood. Archives of Disease in Childhood 43:629–645
     [Google Scholar]
  7. GIMENEZ H. B., KEIR H. M., CASH P. 1987; Immunoblot analysis of the human antibody response to respiratory syncytial virus infection. Journal of General Virology 68:1267–1275
     [Google Scholar]
  8. GRAVES M., GRIFFIN D. E., JOHNSON R. T., HIRSCH R. L., DE SORIANO I. L., ROEDENBECK S., VAISBERG A. 1984; Development of antibody to measles virus polypeptides during complicated and uncomplicated measles virus infections. Journal of Virology 49:409–412
     [Google Scholar]
  9. GRUBER C., LEVINE S. 1983; Respiratory syncytial virus polypeptides. III. The envelope-associated proteins. Journal of General Virology 64:825–832
     [Google Scholar]
  10. GRUBER C., LEVINE S. 1985a; Respiratory syncytial virus polypeptides. IV. The oligosaccharides of the glycoproteins. Journal of General Virology 66:417–432
     [Google Scholar]
  11. GRUBER C., LEVINE S. 1985b; Respiratory syncytial virus polypeptides. V. The kinetics of glycoprotein synthesis. Journal of General Virology 66:1241–1247
     [Google Scholar]
  12. HORNSLETH A., BECH-THOMPSON N., FRIIS B. 1985; Detection of RS virus IgG-subclass-specific antibodies: variation according to age in infants and small children and diagnostic value in RS-virus-infected small infants. Journal of Medical Virology 16:329–335
     [Google Scholar]
  13. HUANG Y. T., COLLINS P. L., WERTZ G. W. 1985; Characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein. Virus Research 2:157–173
     [Google Scholar]
  14. LEVINE S. 1977; Polypeptides of respiratory syncytial virus. Journal of Virology 21:427–431
     [Google Scholar]
  15. LEVINE S., HAMILTON R. 1969; Kinetics of the respiratory syncytial virus growth cycle in HeLa cells. Archiv für die gesamte Virusforschung 28:122–132
     [Google Scholar]
  16. LEVINE S., KLAIBER-FRANCO R., PARADISO P. R. 1987; Demonstration that glycoprotein G is the attachment protein of respiratory syncytial virus. Journal of General Virology 68:2521–2524
     [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
     [Google Scholar]
  18. MACHAMER C. E., HAYES E. C, GOLLOBIN S. D., WESTFALL L. K., ZWEERINK H. J. 1980; Antibodies against the measles matrix polypeptide after clinical infection and vaccination. Infection and Immunity 27:817–825
     [Google Scholar]
  19. MCINTOSH K., FISHAUT J. M. 1980 Immunopathologic mechanisms in lower respiratory disease of infants due to respiratory syncytial virus. Progress in Medical Virology 2694–118 Edited by Melnick J. L. Basel: S. Karger;
     [Google Scholar]
  20. MURPHY B. R., GRAHAM B. S., PRINCE G. A., WALSH E. E., CHANOCK R. M., KARZON D. T., WRIGHT P. F. 1986; Serum and nasal-wash immunoglobulin G and A antibody response of infants and children to respiratory syncytial virus F and G glycoproteins following primary infection. Journal of Clinical Microbiology 23:1009–1014
     [Google Scholar]
  21. NORRBY E., ÖRVELL C, VANDVIK B., CHERRY J. D. 1981; Antibodies against measles virus polypeptides in different disease conditions. Infection and Immunity 34:718–724
     [Google Scholar]
  22. PEEPLES M., LEVINE S. 1979; Respiratory syncytial virus polypeptides: their location in the virion. Virology 95:137–145
     [Google Scholar]
  23. PEMBERTON R. M., CANNON M. J., OPENSHAW P. J. M., BALL L. A., WERTZ G. W., ASKONAS B. A. 1987; Cytotoxic T cell specificity for respiratory syncytial virus proteins: fusion protein is an important target antigen. Journal of General Virology 68:2177–2182
     [Google Scholar]
  24. PRINGLE C. R., SHIRODARIA P. V., GIMENEZ H. B., LEVINE S. 1981; Antigen and polypeptide synthesis by temperature-sensitive mutants of respiratory syncytial virus. Journal of General Virology 54:173–183
     [Google Scholar]
  25. ROSS C. A., PINKERTON I. W., ASSAAD F. A. 1971; Pathogenesis of respiratory syncytial virus diseases in infancy. Archives of Disease in Childhood 46:702–704
     [Google Scholar]
  26. SAMSON A. C R., WILLCOCKS M. M., ROUTLEDGE E. G., MORGAN L. A., TOMS G. L. 1986; A neutralizing monoclonal antibody to respiratory syncytial virus which binds to both F1 and F2 components of the fusion protein. Journal of General Virology 67:1479–1483
     [Google Scholar]
  27. TRUDEL M., NADON F., SEGUIN C, GHOBRIL S., PAYMENT P., TREPANIER P. 1986; Immunovirological studies on human respiratory syncytial virus structural proteins. Canadian Journal of Microbiology 32:15–21
     [Google Scholar]
  28. TRUDEL M., NADON F., SEGUIN C, DIONNE G., LACROIX M. 1987a; Identification of a synthetic peptide as part of a major neutralization epitope of respiratory syncytial virus. Journal of General Virology 68:2273–2280
     [Google Scholar]
  29. TRUDEL M., NADON F., SEGUIN C, PAYMENT P., TALBOT P. J. 1987b; Respiratory syncytial virus fusion glycoprotein: further characterization of a major epitope involved in virus neutralization. Canadian Journal of Microbiology 33:933–938
     [Google Scholar]
  30. TSUTSUMI H., FLANAGAN T. D., OGRA P. L. 1987; Monoclonal antibodies to the large glycoproteins of respiratory syncytial virus: possible evidence for several functional antigenic sites. Journal of General Virology 68:2161–2167
     [Google Scholar]
  31. VAINIONPAA R., MEURMAN O., SARKKINEN H. 1985; Antibody response to respiratory syncytial structural proteins in children with acute respiratory syncytial virus infection. Journal of Virology 53:976–979
     [Google Scholar]
  32. WALSH E. E., HRUSKA J. 1983; Monoclonal antibodies to respiratory syncytial virus proteins. Identification of the fusion protein. Journal of Virology 47:171–177
     [Google Scholar]
  33. WALSH E. E., COTE P. J., FERNIE B. F., SCHLESINGER J. J., BRANDRISS M. W. 1986; Analysis of the respiratory syncytial virus fusion protein using monoclonal and polyclonal antibodies. Journal of General Virology 67:505–513
     [Google Scholar]
  34. WARD K. A., LAMBDEN P. R., OGILVIE M. M., WATT P. J. 1983; Antibodies to respiratory syncytial virus polypeptides and their significance in human infection. Journal of General Virology 64:1867–1876
     [Google Scholar]
  35. WATT P. J., ZARDIS M., LAMBDEN P. R. 1986; Age related IgG subclass response to respiratory syncytial virus fusion protein in infected infants. Clinical and Experimental Immunology 64:503–509
     [Google Scholar]
  36. WERTZ G. W., COLLINS P. L., HUANG Y., GRUBER C, LEVINE S., BALL L. A. 1985; Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proceedings of the National Academy of Sciences, U.S.A 82:4075–4079
     [Google Scholar]
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The Response of Infants with Bronchiolitis to the Proteins of Respiratory Syncytial Virus
J. Gen. Virol. 69, 1229 (1988); https://doi.org/10.1099/0022-1317-69-6-1229
/content/journal/jgv/10.1099/0022-1317-69-6-1229
/content/journal/jgv/10.1099/0022-1317-69-6-1229
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