1887

Abstract

Summary

Virus envelope glycoprotein gVI (gp50) of Aujeszky’s disease virus (ADV) was purified from a Nonidet P40-solubilized lysate of ADV-infected Vero cells by immunoaffinity chromatography using a monoclonal antibody against gVI. Mice immunized by the purified gVI produced virus-neutralizing antibody and were successfully protected against subsequent challenge with ADV.

Keyword(s): ADV , gVI and immunity
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/content/journal/jgv/10.1099/0022-1317-69-6-1411
1988-06-01
2024-05-18
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References

  1. ASHLEY R., MERTZ G., CLARK H., SCHICK M., SALTER D., COREY L. 1985; Humoral immune response to herpes simplex virus type 2 glycoproteins in patients receiving a glycoprotein subunit vaccine. Journal of Virology 56:475–481
    [Google Scholar]
  2. BEN-PORAT T., DEMARCHI J. M., LOMNLCZI B., KAPLAN A. S. 1986; Role of glycoproteins of Pseudorabies virus in eliciting neutralizing antibodies. Virology 154:325–334
    [Google Scholar]
  3. CHOPPIN P. W., SCHEID A. 1980; The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Reviews of Infectious Diseases 2:40–61
    [Google Scholar]
  4. COHEN G. H., KATZE M., HYDREAN-STERN C., EISENBERG R. J. 1978; Type-common CP-1 antigen of herpes simplex virus is associated with a 59,000 molecular-weight envelope glycoprotein. Journal of Virology 27:172–181
    [Google Scholar]
  5. FUKUSHO A., SHIMIZU M., KUBO M., NANBA K., SHIMIZU Y., KONNO S., SUZUKI K., OTAKI T. 1981; The first outbreak of Aujeszky’s disease in swine in Japan. II. Virus isolation. Bulletin of the National Institute of Animal Health 82:5–11 in Japanese
    [Google Scholar]
  6. GALFRÉ G., MILSTEIN C. 1981; Preparation of monoclonal antibodies: strategies and procedures. Methods in Enzymology 73:3–46
    [Google Scholar]
  7. GLORIOSO I. C, LEVINE M., HOLLAND T. C, SZCZESLUL M. S. 1980; Mutant analysis of herpes simplex virus-induced cell surface antigens: resistance to complement-mediated immune cytolysis. Journal of Virology 35:672–681
    [Google Scholar]
  8. GLORIOSO J., SCHRÖDER C H., KUMEL G., SZCZESIUL M., LEVINE M. 1984; Immunogenicity of herpes simplex virus glycoproteins gC and gB and their role in protective immunity. Journal of Virology 50:805–812
    [Google Scholar]
  9. HAMPL H., BEN-PORAT T., EHRLICHER L., HABERMEHL K.-O., KAPLAN A. S. 1984; Characterization of the envelope proteins of Pseudorabies virus. Journal of Virology 52:583–590
    [Google Scholar]
  10. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. LAMB R. A., ETKIND P. R., CHOPPIN P. W. 1978; Evidence for a ninth influenza viral polypeptide. Virology 91:60–78
    [Google Scholar]
  12. LAWMAN M. J. P., COURTNEY R. J., EBERLE R., SCHAFFER P. A., O’HARA M. K., ROUSE B. T. 1980; Cell-mediated immunity to herpes simplex virus: specificity of cytotoxic T cells. Infection and Immunity 30:451–461
    [Google Scholar]
  13. METTENLEITER T. C, SCHREURS C, THIEL H.-J., RZIHA H.-J. 1987; Variability of Pseudorabies virus glycoprotein I expression. Virology 158:141–146
    [Google Scholar]
  14. NORRILD B. 1980; Immunochemistry of herpes simplex virus glycoproteins. Current Topics in Microbiology and Immunology 90:67–106
    [Google Scholar]
  15. PETROVSKIS E. A., TIMMINS J. G., ARMENTROUT M. A., MARCHIOLI C C, YANCEY R. J., POST L. E. 1986; DNA sequence of the gene for Pseudorabies virus gp50, a glycoprotein without N-linked glycosylation. Journal of Virology 59:216–223
    [Google Scholar]
  16. ROBERTS P. L., DUNCAN B. E., RAYBOULD T. J. G., WATSON D. H. 1985; Purification of herpes simplex virus glycoproteins B and C using monoclonal antibodies and their ability to protect mice against lethal challenge. Journal of General Virology 66:1073–1085
    [Google Scholar]
  17. SPEAR P. G., ROIZMAN B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. Journal of Virology 9:143–159
    [Google Scholar]
  18. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  19. VESTERGAARD B. F. 1980; Herpes simplex virus antigens and antibodies: a survey of studies based on quantitative Immunoelectrophoresis. Reviews of Infectious Diseases 2:899–913
    [Google Scholar]
  20. WATHEN M. W., WATHEN L. M. K. 1984; Isolation, characterization, and physical mapping of a Pseudorabies virus mutant containing antigenically altered gp50. Journal of Virology 51:57–62
    [Google Scholar]
  21. WEIS J. H., ENQUIST L. W., SALSTROM J. S., WATSON R. J. 1983; An immunologically active chimeric protein containing herpes simplex virus type 1 glycoprotein D. Nature, London 302:72–74
    [Google Scholar]
  22. ZACHARIUS R. M., ZELL T. E., MORRISON J. H., WOODLOCK J. J. 1969; Glycoprotein staining following electrophoresis on acrylamide gels. Analytical Biochemistry 30:148–152
    [Google Scholar]
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