1887

Abstract

SUMMARY

We have confirmed that two closely related circular RNA molecules previously named grapevine yellow speckle viroid (GYSV) and grapevine viroid 1B (GV1B) are indeed viroids. Electron microscopy after spreading under non-denaturing conditions revealed that GYSV has a rod-like structure typical of viroids. Purified GYSV and GV1B replicated independently in inoculated grapevine seedlings and some of the infected plants developed yellow speckle symptoms indicating that both viroids can cause grapevine yellow speckle disease. Plus-sense RNA transcripts derived from a dimeric GYSV cDNA clone induced yellow speckle symptoms in a grapevine seedling confirming the role of GYSV in the yellow speckle disease. Two oligonucleotide probes were synthesized for the detection of the two related viroids. The probes which could detect each viroid individually were used to assess correlations between the occurrence of these viroids and the incidence of the disease.

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/content/journal/jgv/10.1099/0022-1317-70-12-3411
1989-12-01
2024-05-09
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References

  1. Barlass M., Skene K. G. M., Woodham R. C., Krake L. R. 1982; Regeneration of virus-free grapevines using in vitro apical culture. Annals of Applied Biology 101:291–295
    [Google Scholar]
  2. Hashimoto J., Koganezawa H. 1987; Nucleotide sequence and secondary structure of apple scar skin viroid. Nucleic Acids Research 15:7045–7052
    [Google Scholar]
  3. Koltunow A. M., Rezaian M. A. 1988; Grapevine yellow speckle viroid: structural features of a new viroid group. Nucleic Acids Research 16:849–864
    [Google Scholar]
  4. Koltunow A. M., Rezaian M. A. 1989a; Grapevine viroid 1B, a new member of the apple scar skin viroid group contains the left terminal region of tomato planta macho viroid. Virology 170:575–578
    [Google Scholar]
  5. Koltunow A. M., Rezaian M. A. 1989b; A scheme for viroid classification. Intervirology 30:194–201
    [Google Scholar]
  6. Krake L. R., Woodham R. C. 1983; Grapevine yellow speckle agent implicated in the aetiology of vein banding disease. Vitis 22:40–50
    [Google Scholar]
  7. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning : A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. 1984; Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Research 12:7035–7056
    [Google Scholar]
  9. Mink G. I., Parsons J. L. 1975; Rapid indexing procedures for detecting yellow speckle disease in grapevines. Plant Disease Reporter 59:869–872
    [Google Scholar]
  10. Randles J. W., Hatta T. 1979; Circularity of the ribonucleic acids associated with cadang-cadang disease. Virology 96:47–53
    [Google Scholar]
  11. Rezaian M. A., Koltunow A. M., Krake L. R. 1988; Isolation of three viroids and a circular RNA from grapevines. Journal of General Virology 69:413–122
    [Google Scholar]
  12. Sänger H. L., Klotz G., Reisner D., Gross H. J., Kleinschmidt A. K. 1976; Viroids are single-stranded covalently closed circular RNA molecules existing as highly base paired rod-like structures. Proceedings of the National Academy of SciencesU.S.A 73:3852–3856
    [Google Scholar]
  13. Sano T., Uyeda I., Shikata E., Meshi T., Ohno T., Okada Y. 1985; A viroid-like RNA isolated from grapevine has high sequence homology with hop stunt viroid. Journal of General Virology 66:333–338
    [Google Scholar]
  14. Semancik J. S., Rivera-Bustamante R., Goheen A. C. 1987; Widespread occurrence of viroid-like RNAs in grapevines. American Journal of Enology and Viticulture 38:35–40
    [Google Scholar]
  15. Shanmuganathan N., Fletcher G. 1980; The incidence of grapevine yellow speckle disease in Australian grapevines and the influence of inoculum source on symptom expression. Australian Journal of Agricultural Research 31:327–333
    [Google Scholar]
  16. Szychowski J. A., Goheen A. C., Semancik J. S. 1988; Mechanical transmission and rootstock reservoirs as factors in the widespread distribution of viroids in grapevines. American Journal of Enology and Viticulture 39:213–216
    [Google Scholar]
  17. Taylor R. H., Woodham R. C. 1972; Grapevine yellow speckle – a newly recognized graft-transmissible disease of Vitis . Australian Journal of Agricultural Research 23:447–452
    [Google Scholar]
  18. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of SciencesU.S.A 77:5201–5205
    [Google Scholar]
  19. Visvader J. E., Symons R. H. 1985; Eleven new sequence variants of citrus exocortis viroid and the correlation of sequence with pathogenicity. Nucleic Acids Research 13:2907–2920
    [Google Scholar]
  20. Woodham R. C., Krake L. R. 1982; Grapevine yellow speckle disease – studies on natural spread observed in the field. Vitis 21:337–345
    [Google Scholar]
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