1887

Abstract

SUMMARY

Complementary DNA to the genomic RNA of tomato spotted wilt virus (TSWV) was synthesized and cloned in either pUC19 or gt10. Restriction endonuclease maps were constructed from cDNA clones specific for the S and the M RNA segments, extending 3·0 and 5·4 kbp respectively. The nucleotide sequences of the 3′ and 5′ termini of both RNA molecules were determined. The S and M RNAs contain inverted complementary repeats at their termini, probably involved in RNA replication and in the formation of circular nucleocapsids in virions. The terminal structures of the TSWV genome resemble, in these aspects, those of the Bunyaviridae.

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1989-12-01
2024-05-09
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References

  1. Bishop D. H. L., Calisher C. H., Casals J., Chumakov M. P., Gaidamovich S. Y., Hannoun C., Lvov D. K., Marshall I. D., Oker-Blom N., Pettersson R. F., Porterfield J. S., Russell P. K., Shope R. E., Westaway E. G. 1980; Bunyaviridae. Intervirology 14:125–143
    [Google Scholar]
  2. Clerx-Van Haaster C. M., Bishop D. H. L. 1980; Analysis of the 3′-terminal sequences of snowshoe hare and La Crasse bunyaviruses. Virology 105:564–574
    [Google Scholar]
  3. Clerx-Van Haaster C. M., Clerx J. P. M., Ushuima H., Akashi H., Fuller F., Bishop D. H. L. 1982; The 3′ terminal RNA sequences of bunyaviruses and nairoviruses (Bunyaviridae): evidence of end sequence generic differences within the virus family. Journal of General Virology 61:289–292
    [Google Scholar]
  4. England T. E., Uhlenbeck O. C. 1978; 3′-Terminal labelling of RNA with T4 RNA ligase. Nature, London 275:560–561
    [Google Scholar]
  5. Grunstein J. M., Hogness D. M. 1975; Colony hybridization: a method for isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of SciencesU.S.A 72:3961–3965
    [Google Scholar]
  6. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  7. Huynh T. V., Young R. A., Davies R. W. 1985; Construction and screening of cDNA libraries in λgt10 and λgt11. In DNA Cloning Techniques: A Practical Approach49–78 Glover D. Oxford: IRL Press;
    [Google Scholar]
  8. Ie T. S. 1970; Tomato spotted wilt virus. CMI/AAB Descriptions of Plant Viruses39
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Markwell M. A. K. 1982; A new solid-state reagent to iodinate proteins: 1. conditions for the efficient labeling of antiserum. Analytical Biochemistry 125:427–432
    [Google Scholar]
  11. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  12. Milne R. G., Francki R. I. B. 1984; Should tomato spotted wilt virus be considered as a possible member of the family Bunyaviridae?. Intervirology 22:72–76
    [Google Scholar]
  13. Mohamed N. A. 1981; Isolation and characterization of subviral structures from tomato spotted wilt virus. Journal of General Virology 53:197–206
    [Google Scholar]
  14. Mohamed N. A., Randles J. W., Francki R. I. B. 1973; Protein composition of tomato spotted wilt virus. Virology 56:12–21
    [Google Scholar]
  15. Obijeski I. F., Bishop D. H. L., Palmer E. L., Murphy F. A. 1976; Segmented genome and nucleocapsid of La Crasse virus. Journal of Virology 20:664–675
    [Google Scholar]
  16. Pardigon N., Vialat P., Girard M., Bouloy M. 1982; Panhandles and hairpin structures at the termini of Germiston virus RNAs (bunyavirus). Virology 122:191–197
    [Google Scholar]
  17. Parker M. D., Hewlett M. J. 1981; The 3′-terminal sequences of Uukuniemi and Inkoo virus RNA genome segments. In The Replication of Negative Strand Viruses125–145 Bishop D. H. L., Compans R. W. New York: Elsevier/North-Holland;
    [Google Scholar]
  18. Pettersson R. F., Von Bonsdorff C. H. 1975; Ribonucleoproteins of Uukuniemi virus are circular. Journal of Virology 15:386–392
    [Google Scholar]
  19. Sakimura K. 1962; The present status of thrips-borne viruses. In Biological Transmission of Disease Agents33–40 Maramorosch K. New York: Academic Press;
    [Google Scholar]
  20. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesU.S.A 74:5463–5467
    [Google Scholar]
  21. Schmaljohn C. S., Schmaljohn A. L., Dalrymple J. M. 1987; Hantaan virus M RNA: coding strategy, nucleotide sequence and gene order. Virology 157:31–39
    [Google Scholar]
  22. Strauss E. G., Strauss J. H. 1983; Replication strategies of the single stranded RNA viruses of eukaryotes. Current Topics in Microbiology and Immunology 105:1–98
    [Google Scholar]
  23. Strauss J. H., Strauss E. G. 1988; Evolution of RNA viruses. Annual Review of Microbiology 42:657–683
    [Google Scholar]
  24. Tas P. W. L., Boerjan M. L., Peters D. 1977; The structural proteins of tomato spotted wilt virus. Journal of General Virology 36:267–279
    [Google Scholar]
  25. Van Den Hurk J., Tas P. W. L., Peters D. 1977; The ribonucleic acid of tomato spotted wilt virus. Journal of General Virology 36:81–91
    [Google Scholar]
  26. Van Kammen A., Henstra S., Ie T. S. 1966; Morphology of tomato spotted wilt virus. Virology 30:574–577
    [Google Scholar]
  27. Verkleij F. N., Peters D. 1983; Characterization of a defective form of tomato spotted wilt virus. Journal of General Virology 64:677–686
    [Google Scholar]
  28. Verkleij F. N., De Vries P., Peters D. 1982; Evidence that tomato spotted wilt virus RNA is a positive strand. Journal of General Virology 58:329–338
    [Google Scholar]
  29. Wieslander R. 1979; A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Analytical Biochemistry 98:305–309
    [Google Scholar]
  30. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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