1887

Abstract

SUMMARY

The structures of the rearranged genomic segment 11 of two spontaneous swine rotavirus strains were determined. We found that the rearrangements involved the duplication of normal segment 11 in a head-to-tail orientation, and partial deletions in both monomers. The open reading frame for VP11, the protein encoded by normal segment 11, was maintained. We also showed that the two rearranged genes were transcribed into RNA molecules of the same length as their corresponding genomic segments.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-6-1329
1989-06-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/6/JV0700061329.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-6-1329&mimeType=html&fmt=ahah

References

  1. Agarwal K. L., Brunsted J., Noyes B. E. 1981; A general method for detection and characterization of an mRNA using an oligonucleotide probe. Journal of Biological Chemistry 256:1023–1028
    [Google Scholar]
  2. Auffray C., Rougeon F. 1980; Purification of mouse immunoglobulin heavy-chain mRNAs from total myeloma tumor RNA. European Journal of Biochemistry 107:303–314
    [Google Scholar]
  3. Bellinzoni R. C., Mattion N., Burrone O., Gonzalez S., La Torre J. L., Scodeller E. A. 1987; Isolation of group A swine rotaviruses displaying atypical electropherotypes. Journal of Clinical Microbiology 25:952–954
    [Google Scholar]
  4. Besselaar T. G., Rosenblatt A., Kidd A. H. 1986; Atypical rotavirus from South African neonates. Archives of Virology 87:327–330
    [Google Scholar]
  5. Bishop J. M., Koch G. 1969; Infectious replicative intermediate of poliovirus: purification and characterization. Virology 37:521–534
    [Google Scholar]
  6. Both G. W., Bellamy A. R., Street J. E., Siegman L. J. 1982; A general strategy for cloning double-stranded RNA: nucleotide sequence of the simian-11 rotavirus gene 8. Nucleic Acids Research 11:7075–7088
    [Google Scholar]
  7. Bujarski J. F., Kaesberg P. 1986; Genetic recombination between RNA components of a multipartite plant virus. Nature, London 321:528–531
    [Google Scholar]
  8. Cashdollar L. M., Esparza J., Hudson G. R., Chmelo R., Lee P. W. K., Joklik W. K. 1982; Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proceedings of the National Academy of Sciences, U.S.A 79:7644–7648
    [Google Scholar]
  9. Crespi M., Sánchez D., Tisminetzky S., Bellinzoni R., Bercovich A., Rossi M. S., Corley E., De Mendoza D., Hopp E., Zorzopulos J., Frasch C., Baralle F., Burrone O. 1986; Molecular cloning of rotavirus genome. Medicina, Buenos Aires 46:693–697
    [Google Scholar]
  10. Espejo R. T., Muñoz O., Serafin F., Romero P. 1980; Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences. Infection and Immunity 27:351–354
    [Google Scholar]
  11. Estes M. K., Graham D. Y., Gerba C. P., Smith E. M. 1979; Simian rotavirus SA11 replication in cell cultures. Journal of Virology 31:810–815
    [Google Scholar]
  12. Estes M. K., Graham D. Y., Dimitrov D. H. 1984; The molecular epidemiology of rotavirus gastroenteritis. Progress in Medical Virology 29:1–22
    [Google Scholar]
  13. Gubler U., Hoffman J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  14. Henikoff S. 1984; Unidirectional digestion with exonuclease HI creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  15. Hundley F., Biryahwaho B., Gow M., Desselberger U. 1985; Genome rearrangements of bovine rotavirus after serial passage at high multiplicity of infection. Virology 143:88–103
    [Google Scholar]
  16. Hundley F., Mc Ire M., Clark B., Beards G., Wood D., Chrystie I., Desselberger U. 1987; Heterogeneity of genome rearrangements in rotaviruses isolated from a chronically infected immunodeficient child. Journal of Virology 61:3365–3372
    [Google Scholar]
  17. Imai M., Richardson M. A., Ikegami N., Shatkin A. J., Furuichi Y. 1983; Molecular cloning of double-stranded RNA virus genomes. Proceedings of the National Academy of Sciences, U.S.A 80:373–377
    [Google Scholar]
  18. Kirkegaard K., Baltimore D. 1986; The mechanism of RNA recombination in poliovirus. Cell 47:433–443
    [Google Scholar]
  19. Mccrae M. A., Mccorquodale J. G. 1983; Molecular biology of rotaviruses. V. Terminal structure of viral RNA species. Virology 126:204–212
    [Google Scholar]
  20. Makino S., Keck J. G., Stohlman S. A., Lai M. M. C. 1986; High-frequency RNA recombination of murine coronavirus. Journal of Virology 57:729–737
    [Google Scholar]
  21. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Mason B. B., Graham D. Y., Estes M. K. 1980; In vitro transcription and translation of simian rotavirus SA11 gene products. Journal of Virology 33:1111–1121
    [Google Scholar]
  23. Mattion N., González S. A., Burrone O., Bellinzoni R., La Torre J. L., Scodeller E. A. 1988; Rearrangement of genomic segment 11 in two swine rotavirus strains. Journal of General Virology 69:695–698
    [Google Scholar]
  24. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–580
    [Google Scholar]
  25. Mitchell D. B., Both G. W. 1988; Simian rotavirus SA11 contains overlapping reading frames. Nucleic Acids Research 16:6244
    [Google Scholar]
  26. Pedley S., Hundley F., Chrystie I., Mccrae M. A., Desselberger U. 1984; The genomes of rotaviruses isolated from chronically infected immunodeficient children. Journal of General Virology 65:1141–1150
    [Google Scholar]
  27. Pocock D. H. 1987; Isolation and characterization of two group A rotaviruses with unusual genome profiles. Journal of General Virology 68:653–660
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  29. Smith R. E., Zweerink H. J., Joklik W. K. 1969; Polypeptide components of virions, top components and cores of reovirus type 3. Virology 39:791–810
    [Google Scholar]
  30. Tanaka T. N., Conner M. E., Graham D. Y., Estes M. K. 1988; Molecular characterization of three rabbit rotavirus strains. Archives of Virology 98:253–265
    [Google Scholar]
  31. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.SA 77:5201–5205
    [Google Scholar]
  32. Thouless M. E., Di Giacomo R. F., Neuman D. S. 1986; Isolation of two lapine rotaviruses: characterization of the subgroup, serotype and RNA electropherotype. Archives of Virology 89:161–170
    [Google Scholar]
  33. Virtanen A., Pettersson U. 1985; Organization of early region IB of human adenovirus type 2: identification of four differentially spliced mRNAs. Journal of Virology 54:383–391
    [Google Scholar]
  34. Ward C. W., Azad A. A., Dyall-smith M. L. 1985; Structural homologies between RNA segments 10 and 11 from UK bovine, simian SAU, and human Wa rotaviruses. Virology 144:328–336
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-6-1329
Loading
/content/journal/jgv/10.1099/0022-1317-70-6-1329
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error