1887

Journal of General Virology header logo

Volume 71, Issue 1

The Use of General Primers in the Polymerase Chain Reaction Permits the Detection of a Broad Spectrum of Human Papillomavirus Genotypes Free

Abstract

A novel polymerase chain reaction (PCR) method was developed that permits the detection of 11 different human papillomavirus (HPV) genotypes using two general primer sets. By computer-assisted sequence analysis, two pairs of general primers were selected from the conserved L1 open reading frame and tested in the PCR on a set of cloned HPV genotypes. Experimental analysis showed that up to three mismatches between primers and target DNA did not influence the efficiency of the assay. The use of these primers in the PCR enabled the detection of HPV genotypes HPV-1a, −6, −8, −11, −13, −16, −18, −30, −31, −32 and −33, and was also successfully applied to well characterized cervical carcinoma cell lines and clinical samples. For the HPV types tested sub-picogram amounts of cloned DNA could be detected after general primer-mediated PCR and subsequent hybridization. The specificity of the amplification products was confirmed by blot hybridization procedures and restriction enzyme digestion. The results indicate that this PCR method can be a powerful tool for identifying novel HPV genotypes in dysplasias and squamous cell carcinomas suspected of having an HPV aetiology.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1990
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-1-173
1990-01-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/1/JV0710010173.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-1-173&mimeType=html&fmt=ahah

References

  1. Abramson A. L., Brandsma J. L., Steinberg B. M., Winkler B. 1985; Verrucous carcinoma of the larynx. Possible human papillomavirus etiology. Archives of Otolaryngology 111:709–715
     [Google Scholar]
  2. Boshart M., Gissmann L., Ikenberg H., Kleinheinz A., Scheurlen W., Zur Hausen H. 1984; A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO Journal 3:1151–1157
     [Google Scholar]
  3. Brandsma J. L., Abramson A. L. 1989; Association of papillomavirus with cancers of the head and neck. Archives of Otolaryngology and Head and Neck Surgery 115:621–625
     [Google Scholar]
  4. Brandsma J. L., Steinberg B. M., Abramson A. L., Winkler B. 1986; Presence of human papillomavirus type 16 related sequences in verrucous carcinoma of the larynx. Cancer Research 46:2185–2188
     [Google Scholar]
  5. Byrne J. C., Tsao M.-S., Fraser R. S., Howley P. M. 1987; Human papillomavirus-11 DNA in a patient with laryngotracheo-bronchial papillomatosis and metastatic squamous-cell carcinoma of the lung. New England Journal of Medicine 317:873–878
     [Google Scholar]
  6. Carman W. F., Kidd A. H. 1989; An assessment of optimal conditions for amplification of HIV cDNA using Thermus aquaticus polymerase. Journal of Virological Methods 23:277–290
     [Google Scholar]
  7. Cole S. T., Danos O. 1987; Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products. Journal of Molecular Biology 193:599–608
     [Google Scholar]
  8. Cole S. T., Streeck R. 1986; Genome organization and nucleotide sequence of human papillomavirus type 33, which is associated with cervical cancer. Journal of Virology 58:991–995
     [Google Scholar]
  9. Danos O., Katinka M., Yaniv M. 1982; Human papillomavirus la complete DNA sequence: a novel type of genome organization among Papovaviridae. EMBO Journal 1:231–236
     [Google Scholar]
  10. Danos O., Engel L. W., Chen E. Y., Yaniv M., Howley P. M. 1983; Comparative analysis of the human type la and bovine type 1 papillomavirus genomes. Journal of Virology 46:557–566
     [Google Scholar]
  11. Dartmann K., Schwarz E., Gissmann L., Zur Hausen H. 1986; The nucleotide sequence and genome organization of human papillomavirus type 11. Virology 151:124–130
     [Google Scholar]
  12. De Villiers E.-M., Weidauer H., Otto H., Zur Hausen H. 1985; Papillomavirus DNA in human tongue carcinomas. International Journal of Cancer 36:575–578
     [Google Scholar]
  13. Dürst M., Gissmann L., Ikenberg H., Zur Hausen H. 1983; A papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proceedings of the National Academy of Sciences, U.S.A 80:3812–3815
     [Google Scholar]
  14. Fuchs P. G., Iftner T., Weninger J., Pfister H. 1986; Epidermodysplasiaverruciformis-associated human papillomavirus 8: genomic sequence and comparative analysis. Journal of Virology 58:626–634
     [Google Scholar]
  15. Giri I., Danos O. 1986; Papillomavirus genomes.From sequence data to biological properties. Trends in Genetics 2:227–232
     [Google Scholar]
  16. Goldsborough M. D., Di-Silvestre D., Temple G. F., Lorincz A. T. 1989; Nucleotide sequence of human papillomavirus type 31: a cervical neoplasia-associated virus. Virology 171:306–311
     [Google Scholar]
  17. Kahn T., Schwarz E., Zur Hausen H. 1986; Molecular cloning and characterization of the DNA of a new human papillomavirus (HPV30) from a laryngeal carcinoma. International Journal of Cancer 37:61–65
     [Google Scholar]
  18. Löning T., Ikenberg H., Becker J., Gissmann L., Hoepfer I., Zur Hausen H. 1985; Analysis of oral papillomas, leukoplakias, and invasive carcinomas for human papillomavirus type related DNA. Journal of Investigative Dermatology 84:417–420
     [Google Scholar]
  19. Melchers W., Van Den Brule A., Walboomers J., De Bruin M., Burger M., Herbrink P., Meijer C., Lindeman J., Quint W. 1989; Increased detection rate of human papillomavirus in cervical scrapes by the polymerase chain reaction as compared to modified FISH and Southern-blot analysis. Journal of Medical Virology 27:329–335
     [Google Scholar]
  20. Newton C. R., Graham A., Heptinstall L. E., Powel S. J., Summers C., Kalsheker N., Smith J. C., Markham A. F. 1989; Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Research 17:2503–2516
     [Google Scholar]
  21. Queen C., Korn L. J. 1984; A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Research 12:581–599
     [Google Scholar]
  22. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Ehrlich H. A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
     [Google Scholar]
  23. Schwarz E., Dürst M., Demankowski C., Lattermann O., Zech R., Wolfsperger E., Suhai S., Zur Hausen H. 1983; DNA sequence and genome organization of genital human papillomavirus type 6b. EMBO Journal 2:2341–2348
     [Google Scholar]
  24. Schwarz E., Freese U. K., Gissmann L., Mayer W., Roggenbuck B., Stremlau A., Zur Hausen H. 1985; Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature; London: 314111–114
     [Google Scholar]
  25. Seedorf K., Krämmer G., Dürst M., Suhai S., Röwekamp W. G. 1985; Human papillomavirus type 16 DNA sequence. Virology 145:181–185
     [Google Scholar]
  26. Stremlau A., Gissmann L., Ikenberg H., Stark M., Bannasch P., Zur Hausen H. 1985; Human papillomavirus type 16 related DNA in an anaplastic carcinoma of the lung. Cancer 55:1737–1740
     [Google Scholar]
  27. Van Den Brule A. J. C., Claas H. C. J., Du Maine M., Melchers W. J. G., Helmerhorst T., Quint W. G. V., Lindeman J., Meijer C. J. L. M., Walboomers J. M. M. 1989; Use of anticontamination primers in the polymerase chain reaction for the detection of human papilloma virus genotypes in cervical scrapes and biopsies. Journal of Medical Virology 29:20–27
     [Google Scholar]
  28. Walboomers J. M. M., Melchers W. J. G., Mullink H., Meijer C. J. L. M., Struyk A., Quint W. G. J., Van Der Noordaa J., Ter Schegget J. 1988; Sensitivity of in situ detection with biotinylated probes of human papilloma virus type 16 DN A in frozen tissue sections of squamous cell carcinomas of the cervix. American Journal of Pathology 131:587–594
     [Google Scholar]
  29. Yee C., Krishnan-Hewlett I., Baker C. C., Schlegel R., Howley P. M. 1985; Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. American Journal of Pathology 119:361–366
     [Google Scholar]
  30. Zachow K. R., Ostrow R. S., Faras A. J. 1987; Nucleotide sequence and genome organization of human papillomavirus type 5. Virology 158:251–254
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-1-173
Loading
The Use of General Primers in the Polymerase Chain Reaction Permits the Detection of a Broad Spectrum of Human Papillomavirus Genotypes
J. Gen. Virol. 71, 173 (1990); https://doi.org/10.1099/0022-1317-71-1-173
/content/journal/jgv/10.1099/0022-1317-71-1-173
/content/journal/jgv/10.1099/0022-1317-71-1-173
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error