1887

Abstract

A cDNA library was constructed in gt10 using mRNA purified from cells infected with parainfluenza virus type 2 (PIV2). Virus-specific clones were identified by screening the library with P-labelled cDNA probes made from randomly primed vRNA. Clones containing the haemagglutinin-neuraminidase (HN) gene were identified by sequence comparisons with known parainfluenza virus HN gene sequences. The largest HN clone isolated had a nucleic acid sequence of 2065 bp with a single long open reading frame encoding a protein of 571 amino acids. The HN protein has nine predicted glycosylation sites and an amino-terminal membrane-spanning region. The PIV2 HN protein shares 43 % amino acid identity with the HN protein of simian virus 5 and 40% with mumps virus, 30% of the amino acids being common to all three viruses.

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1990-05-01
2024-04-26
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References

  1. Elango N., Coligan J. E., Jambou R. C., Venkatesan S. 1986; Human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mRNA and limited amino acid sequence of CnBr fragments of the purified protein. Journal of Virology 57:481–489
    [Google Scholar]
  2. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabeling DNA restriction endonuclease fragments to a high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  3. Goswami K. K. A., Russell W. C. 1983; Monoclonal antibodies against human paramyxovirus type 3 and against SV5 virus: preparation and preliminary characterization. Journal of General Virology 64:1663–1672
    [Google Scholar]
  4. Goswami K. K. A., Lange L. S., Mitchell D. N., Cameron K. R., Russell W. C. 1984; Does simian virus 5 infect humans?. Journal of General Virology 65:1295–1303
    [Google Scholar]
  5. Goswami K. K. A., Randall R. E., Lange L. S., Russell W. C. 1987; Antibodies against the paramyxovirus SV5 in the cerebrospinal fluids of some multiple sclerosis patients. Nature; London: 327244–247
    [Google Scholar]
  6. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for the isolation of cloned cDNAs that contain a specific gene. Proceedings of the National Academy of Sciences U.S.A.: 723961–3965
    [Google Scholar]
  7. Hiebert S. W., Paterson R. G., Lamb R. A. 1985; Hemagglutinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal membrane anchor. Journal of Virology 54:1–6
    [Google Scholar]
  8. Hiebert S. W., Richardson C. D., Lamb R. A. 1988; Cell surface expression and orientation in membranes of the 44-amino-acid SH protein of simian virus 5. Journal of Virology 62:2347–2357
    [Google Scholar]
  9. Hsiung G. D. 1972; Parainfluenza-5 virus. Infection of man and animals. Progress in Medical Virology 14:241–274
    [Google Scholar]
  10. Hyunh T. V., Young R. A., Davis R. W. 1985; Constructing and screening cDNA libraries in λgt10 and λgtll. In DNA Cloning Techniques: A Practical Approach pp 49–78 Glover D. Edited by Oxford: IRL Press;
    [Google Scholar]
  11. Ito Y., Tsurudome M., Hishiyama M., Yamada A. 1987; Immunological interrelationships among human and non-human paramyxoviruses revealed by immunoprecipitation. Journal of General Virology 68:1289–1297
    [Google Scholar]
  12. Kingsbury D. W., Bratt M. A., Choppin P. W., Hanson R. P., Hosaka Y., Ter Meulen V., Norrby E., Plowright W., Rott R., Wunner W. H. 1978; Paramyxoviridae. Intervirology 10:137–152
    [Google Scholar]
  13. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  14. Manfioletti G., Schneider C. 1988; A new and fast method for preparing high quality lambda DNA suitable for sequencing. Nucleic Acids Research 16:2873–2884
    [Google Scholar]
  15. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold-Spring Harbor Laboratory;
    [Google Scholar]
  16. Margalit H., Spouge J. L., Cornette J. L., Cease K. B., Delisi C., Berzofsky J. A. 1987; Prediction of immunodominant helper T cell antigenic sites from the primary sequence. Journal of Immunology 138:2213–2229
    [Google Scholar]
  17. Neuberger A., Gottschalk A., Marshall R. D., Spiro R. G. 1972; Carbohydrate-peptide linkages in glycoproteins and methods for their elucidation. In Glycoproteins pp 450–485 Gottschalk A. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  18. Paterson R. G., Harris J. R. T., Lamb R. A. 1984; Analysis and gene assignment of a paramyxovirus, simian virus 5. Virology 138:310–323
    [Google Scholar]
  19. Pringle C. R. 1987; Paramyxoviruses and disease. In The Molecular Basis of Virus Disease, Society for General Microbiology Symposium 40 pp 51–90 Russell W. C., Almond J. W. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  20. Randall R. E., Souberbielle B. E. 1990; Presentation of virus antigens for the induction of protective immunity. In Control of Virus Diseases Society for General Microbiology Symposium 45 Dimmock N. J., Griffiths P. D., Madeley C. R. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  21. Randall R. E., Young D. F. 1988; Comparison between parainfluenza virus type 2 and simian virus 5: monoclonal antibodies reveal major antigenic differences. Journal of General Virology 69:2051–2060
    [Google Scholar]
  22. Randall S. K., Gelfand D. H., Stoffell S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  23. Rydbeck R., Löve A., Örvell C. 1988; Parainfluenza virus type 2 haemagglutinin-neuraminidase glycoprotein characterized with monoclonal antibodies. Journal of General Virology 69:931–935
    [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A.: 745463–5467
    [Google Scholar]
  25. Southern J. A., Precious B., Randall R. E. 1990; Two nontemplated nucleotide additions are required to generate the P mRNA of parainfluenza virus type 2 since the RNA genome encodes protein V. Virology in press
    [Google Scholar]
  26. Spriggs M. K., Olmsted R. A., Venkatesan S., Coligan J. E., Collins P. L. 1986; Fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-action site, and comparison with other paramyxoviruses. Virology 152:241–251
    [Google Scholar]
  27. Staden R. 1982a; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  28. Staden R. 1982b; An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Research 10:2951–2961
    [Google Scholar]
  29. Suzu S., Sakai Y., Shioda T., Shibuta H. 1987; Nucleotide sequence of bovine parainfluenza 3 virus genome: the genes of the F and HN glycoproteins. Nucleic Acids Research 15:2945–2957
    [Google Scholar]
  30. Thomas S. M., Lamb R. A., Paterson R. G. 1988; Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. Cell 54:891–902
    [Google Scholar]
  31. Tsurudome M., Nishio M., Komada H., Bando H., Ito Y. 1989; Extensive antigenic diversity among human parainfluenza type 2 virus isolates and immunological relationships among paramyxoviruses revealed by monoclonal antibodies. Virology 171:34–48
    [Google Scholar]
  32. Vandvik B., Norrby E. 1989; Paramyxovirus SV5 and multiple sclerosis. Nature; London: 338769–771
    [Google Scholar]
  33. Waxam N. M., Aronowski J., Server A. C., Wolinsky J. S., Smith J. A., Goodman H. M. 1988; Sequence determination of the mumps virus HN gene. Virology 164:318–325
    [Google Scholar]
  34. Wright P. F. 1984; Parainfluenza viruses. In Textbook of Human Virology pp 299–309 Belshe R. B. Edited by Littleton, Massachusetts: PSG Publishing Company;
    [Google Scholar]
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