1887

Abstract

Monoclonal antibodies (MAbs) to the haemagglutinin—neuraminidase (HN), fusion (F) and matrix (M) proteins of Newcastle disease virus were prepared and characterized. At least three non-overlapping or partially overlapping antigenic sites were delineated on the HN, three on the F and three on the M proteins by competitive binding assays. Antigenic sites on the HN and F proteins roughly represented functional domains defined by serological tests. Two antigenic sites on the F protein were involved in virus neutralizing and haemolysis-inhibiting activity. These antigenic determinants were readily affected by treatment with certain surfactants and acetone. Proteolytic cleavage of the HN and F proteins was associated with conformational change, revealed by altered reactivity with MAbs and by altered topological arrangements of some epitopes. None of the anti-M MAbs inhibited any biological activities of the virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-5-1189
1990-05-01
2024-04-30
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/5/JV0710051189.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-5-1189&mimeType=html&fmt=ahah

References

  1. Abenes G., Kida H., Yanagawa R. 1986; Antigenic mapping and functional analysis of the F protein of Newcastle disease virus using monoclonal antibodies. Archives of Virology 90:97–110
    [Google Scholar]
  2. Chambers P., Millar N. S., Emmerson P. T. 1986; Nucleotide sequence of the gene encoding the fusion glycoprotein of Newcastle disease virus. Journal of General Virology 67:2685–2694
    [Google Scholar]
  3. Compans R. W., Klenk H.-D. 1979; Viral membranes. In Comprehensive Virology 13 pp 293–407 Fraenkel-Conrat H., Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  4. Erdei J., Erdei J., Bachir K., Kaleta E. F., Shortridge K. F., Lomniczi B. 1987; Newcastle disease vaccine (La Sota) strain specific monoclonal antibody. Archives of Virology 96:265–269
    [Google Scholar]
  5. Ey P. L., Prowse S. J., Jenkin C. R. 1978; Isolation of pure IgGi, IgG2a and IgG2b immunoglobulins from mouse serum using Protein A-Sepharose. Immunochemistry 15:429–436
    [Google Scholar]
  6. Iorio R. M., Bratt M. A. 1983; Monoclonal antibodies to Newcastle disease virus: delineation of four epitopes on the HN glycoprotein. Journal of Virology 48:440–450
    [Google Scholar]
  7. Iorio R. M., Bratt M. A. 1984a; Monoclonal antibodies as functional probes of the HN glycoprotein of Newcastle disease virus: antigenic separation of the hemagglutinating and neuraminidase sites. Journal of Immunology 133:2215–2219
    [Google Scholar]
  8. Iorio R. M., Bratt M. A. 1984b; Neutralization of Newcastle disease virus by monoclonal antibodies to the hemagglutinin- neuraminidase glycoprotein: requirement for antibodies to four sites for complete neutralization. Journal of Virology 51:445–451
    [Google Scholar]
  9. Iorio R. M., Borgman J. B., Glickman R. L., Bratt M. A. 1986; Genetic variation within a neutralizing domain on the haemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. Journal of General Virology 67:1393–1403
    [Google Scholar]
  10. Ishida N., Homma M. 1978; Sendai virus. In Advances in Virus Research pp 349–383 Lauffer M. A., Bang F. B., Maramorosch K., Smith K. M. Edited by New York: Academic Press;
    [Google Scholar]
  11. Kohama T., Garten W., Klenk H.-D. 1981; Changes in conformation and charge paralleling proteolytic activation of Newcastle disease virus glycoproteins. Virology 111:364–376
    [Google Scholar]
  12. Kohama T., Sato T. A., Kobune F., Sugiura A. 1985; Maturation of measles virus hemagglutinin glycoprotein. Archives of Virology 85:257–268
    [Google Scholar]
  13. Kohama T., Fukuda A., Sugiura A. 1986; Effect of carboxylic ionophores on measles virus hemagglutinin protein. Archives of Virology 89:213–223
    [Google Scholar]
  14. Lamb R. A., Etkind P. R., Choppin P. W. 1978; Evidence for a ninth influenza viral polypeptide. Virology 91:60–78
    [Google Scholar]
  15. Long L., Portetelle D., Ghysdael J., Gonze M., Burny A., Meulemans G. 1986; Monoclonal antibodies to hemagglutinin- neuraminidase and fusion glycoproteins of Newcastle disease virus: relationship between glycosylation and reactivity. Journal of Virology 57:1198–1202
    [Google Scholar]
  16. Millar N. S., Chambers P., Emmerson P. T. 1988; Nucleotide sequence of the fusion and haemagglutinin-neuraminidase glycoprotein genes of Newcatle disease virus, strain Ulster: molecular basis for variations in pathogenicity between strains. Journal of General Virology 69:613–620
    [Google Scholar]
  17. Morrison T. G., Peeples M. E., McGinnes L. W. 1987; Conformational change in a viral glycoprotein during maturation due to disulfide bond disruption. Proceedings of the National Academy of Sciences U.S.A.: 841020–1024
    [Google Scholar]
  18. Nagai Y., Klenk H.-D. 1977; Activation of precursors to both glycoproteins of Newcastle disease virus by proteolytic cleavage. Virology 77:125–134
    [Google Scholar]
  19. Nagai Y., Klenk H.-D., Rott R. 1976; Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508
    [Google Scholar]
  20. Nishikawa K., Isomura S., Suzuki S., Watanabe E., Hamaguchi M., Yoshida T., Nagai Y. 1983; Monoclonal antibodies to the HN glycoprotein of Newcastle disease virus. Biological characterization and use for strain comparisons. Virology 130:318–330
    [Google Scholar]
  21. Nishikawa K., Morishima T., Toyoda T., Miyadai T., Yokochi T., Yoshida T., Nagai Y. 1986; Topological and operational delineation of antigenic sites on the HN glycoprotein of Newcastle disease virus and their structural requirements. Journal of Virology 60:987–993
    [Google Scholar]
  22. Nishikawa K., Hanada N., Morishima T., Yoshida T., Hamaguchi M., Toyoda T., Nagai Y. 1987; Antigenic characterization of the internal proteins of Newcastle disease virus by monoclonal antibodies. Virus Research 1:83–92
    [Google Scholar]
  23. Russell P. H. 1984; Newcastle disease virus: the effect of monoclonal antibody in the overlay on virus penetration and the immunoselection of variants. Journal of General Virology 65:795–798
    [Google Scholar]
  24. Russell P. H. 1986; The synergistic neutralization of Newcastle disease virus by two monoclonal antibodies to its haemagglutinin- neuraminidase protein. Archives of Virology 90:135–144
    [Google Scholar]
  25. Russell P. H., Alexander D. J. 1983; Antigenic variation of Newcastle disease virus strains detected by monoclonal antibodies. Archives of Virology 75:243–253
    [Google Scholar]
  26. Russell P. H., Griffiths P. C., Goswami K. K. A., Alexander D. J., Cannon M. J., Russell W. C. 1983; The characterization of monoclonal antibodies to Newcastle disease virus. Journal of General Virology 64:2069–2072
    [Google Scholar]
  27. Scheid A., Choppin P. W. 1977; Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses. Virology 80:54–66
    [Google Scholar]
  28. Schuy W., Garten W, Klenk H.-D. 1984; The carboxyterminus of the hemagglutinin-neuraminidase of Newcastle disease virus is exposed at the surface of the viral envelope. Virus Research 1:415–426
    [Google Scholar]
  29. Seto J. T. 1966; Functional significance of sialidase during influenza virus multiplication. Virology 30:731–737
    [Google Scholar]
  30. Toyoda T., Gotoh B., Sakaguchi T., Kida H., Nagai Y. 1988; Identification of amino acids relevant to three antigenic determinants on the fusion protein of Newcastle disease virus that are involved in fusion inhibition and neutralization. Journal of Virology 62:4427–4430
    [Google Scholar]
  31. Tozawa H., Komatsu H., Ohkata K., Nakajima T., Watanabe M., Tanaka Y., Arifuku M. 1986; Neutralizing activity of the antibodies against two kinds of envelope glycoproteins of Sendai virus. Archives of Virology 91:145–161
    [Google Scholar]
  32. Umino Y., Kohama T., Kohase M., Sugiura A., Klenk H.-D., Rott R. 1984; Biological functions of monospecific antibodies to envelope glycoproteins of Newcastle disease virus. Archives of Virology 81:53–65
    [Google Scholar]
  33. Umino Y., Sato T. A., Katow S., Matsuno T., Sugiura A. 1985; Monoclonal antibodies directed to El glycoprotein of rubella virus. Archives of Virology 83:33–42
    [Google Scholar]
  34. Umino Y., Kohama T., Kohase M., Sugiura A., Klenk H.-D., Rott R. 1987; Protective effect of antibodies to two viral envelope glycoproteins on lethal infection with Newcastle disease virus. Archives of Virology 94:97–107
    [Google Scholar]
  35. Umino Y., Kohama T., Sato T. A., Sugiura A. 1990; Protective effect of monoclonal antibodies to Newcatle disease virus in passive immunization. Journal of General Virology 71:1199–1203
    [Google Scholar]
  36. Wilson M. B., Nakane P. K. 1978; Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In Immunofluorescence and Related Staining Techniques pp 215–224 Knapp W., Holubar K., Wick G. Edited by Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-5-1189
Loading
/content/journal/jgv/10.1099/0022-1317-71-5-1189
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error