1887

Abstract

The Nla-like protein of plum pox virus is a protease with high sequence specificity that is autocatalytically released from the viral polyprotein. In order to determine whether the protease is active in trans we constructed a fusion protein consisting of the C-terminal region of the plum pox virus polyprotein and the staphylococcal Protein A. The authentic protease recognition sequence Asn-Val-Val-Val-His- Gln-Ala occurs in the centre of this protein fusion. This protein was cleaved specifically by extracts of plum pox virus-infected plants due to the strong activity of the viral protease making it a useful tool for diagnostic purposes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-7-1623
1990-07-01
2024-05-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/7/JV0710071623.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-7-1623&mimeType=html&fmt=ahah

References

  1. Carrington J. C., Dougherty W. G. 1987; Small nuclear inclusion protein encoded by a plant potyvirus is a protease. Journal of Virology 61:2540–2548
    [Google Scholar]
  2. Dougherty W. G., Carrington J. C. 1988; Expression and function of potyviral gene products. Annual Review of Phytopathology 26:123–143
    [Google Scholar]
  3. Garcia J. A., Riechmann J. L., Lain S. 1989a; Artificial cleavage site recognized by plum pox potyvirus protease in Escherichia coli . Journal of Virology 63:2457–2460
    [Google Scholar]
  4. Garcia J. A., Riechmann J. L., Lain S. 1989b; Proteolytic activity of the plum pox potyvirus NIa-like protein in Escherichia coli . Virology 170:362–369
    [Google Scholar]
  5. Hellmann G. M., Shaw J. G., Rhoads R. E. 1988; In vitro analysis of tobacco vein mottling virus NIa cistron: evidence for a virus-encoded protease. Virology 163:554–562
    [Google Scholar]
  6. Maiss E., Timpe U., Brisske A., Jelkmann W., Casper R., Himmler G., Mattanovich D., Katinger H. W. D. 1989; The complete nucleotide sequence of plum pox virus RNA. Journal of General Virology 70:513–524
    [Google Scholar]
  7. Mattanovich D., Himmler G., Laimer M., Maiss E., Regner F., Da Camara Machado A., Hanzer V., Casper R., Katinger H. 1988; Expression of the plum pox virus coat protein region in Escherichia coli . Virus Genes 2:119–127
    [Google Scholar]
  8. Nilsson B., Abrahmsen L., Uhlen M. 1985; Immobilization and purification of enzymes with staphylococcal protein A fusion vectors. EMBO Journal 4:1075–1080
    [Google Scholar]
  9. Wellink J., Van Kammen A. 1988; Proteases involved in the processing of viral polyproteins. Archives of Virology 98:1–26
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-7-1623
Loading
/content/journal/jgv/10.1099/0022-1317-71-7-1623
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error