1887

Abstract

We have determined the genomic location and nucleotide sequence of the equine herpesvirus 4 thymidine kinase (TK) gene. The gene is positioned at approximately 0·48 map units within the long unique component of the genome and is flanked by genes encoding a herpes simplex virus 1 (HSV-1) UL24 homologue and glycoprotein H. The predicted protein is composed of 352 amino acids, has an of 38 800 and exhibits 36 % identity to the predicted TK of HSV-1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-8-1801
1990-08-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/8/JV0710081801.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-8-1801&mimeType=html&fmt=ahah

References

  1. Allen G. P., Bryans J. T. 1986; Molecular epizootiology, pathogenesis and prophylaxis of equine herpesvirus-1 infections. In Progress in Veterinary Microbiology and Immunology 2 pp Pandey R. Edited by Basel: S. Karger;
    [Google Scholar]
  2. Baer R., Bankier A. T., Biggin M. D., Deininger P. L., Farrell P. J., Gibson T. J., Hatfull G., Hudson G. S., Satchwell S. C., Seguin C., Tuffnell P. S., Barrell B. G. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature; London: 310207–211
    [Google Scholar]
  3. Corden J., Wasylyk B., Buchwalder A., Sassone-Corsi P., Kedinger C., Chambon P. 1980; Promoter sequences of eukaryotic protein-coding genes. Science 2091406–1414
    [Google Scholar]
  4. Cullinane A. A., Rixon F. J., Davison A. J. 1988; Characterization of the genome of equine herpesvirus 1 subtype 2. Journal of General Virology 69:1575–1590
    [Google Scholar]
  5. Darby G., Larder B. A., Inglis M. M. 1986; Evidence that the ‘active centre’ of the herpes simplex virus thymidine kinase involves an interaction between three distinct regions of the polypeptide. Journal of General Virology 67:753–758
    [Google Scholar]
  6. Davison A. J., Scott J. E. 1986; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
    [Google Scholar]
  7. Efstathiou S., Kemp S., Darby G., Minson A. C. 1989; The role of herpes simplex virus type 1 thymidine kinase in pathogenesis. Journal of General Virology 70:869–879
    [Google Scholar]
  8. Field H. J., Wildy P. 1978; The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice. Journal of Hygiene 81:267–277
    [Google Scholar]
  9. Gentry G. A. 1985; Locating a nucleotide-binding site in the thymidine kinase of vaccinia virus and of herpes simplex virus by scoring triply aligned protein sequences. Proceedings of the National Academy of Sciences U.S.A.: 826815–6819
    [Google Scholar]
  10. Graham F. L., Van Der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  11. Honess R. W., Craxton M. A., Williams L., Gompels U. A. 1989; A comparative analysis of the sequence of the thymidine kinase gene of a gammaherpesvirus, herpesvirus saimiri. Journal of General Virology 70:3003–3013
    [Google Scholar]
  12. Jacobson J. G., Martin S. L., Coen D. M. 1989; A conserved open reading frame that overlaps the herpes simplex virus thymidine kinase gene is important for viral growth in cell culture. Journal of Virology 63:1839–1843
    [Google Scholar]
  13. Kit S. 1985a; Thymidine kinase. Microbiological Sciences 2:369–375
    [Google Scholar]
  14. Kit S. 1985b US Patent Application4514497
    [Google Scholar]
  15. Kit S., Kit M., Qavi H., Trkula D., Otsuka H. 1983; Nucleotide sequence of the herpes simplex virus type 2 (HSV-2) thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide and its comparison with the HSV-1 thymidine kinase gene. Biochimica et biophysica acta 741:158–170
    [Google Scholar]
  16. Kit S., Kit M., Pirtle E. C. 1985a; Attenuated properties of thymidine kinase-negative deletion mutants of pseudorabies virus. American Journal of Veterinary Research 46:1359–1367
    [Google Scholar]
  17. Kit S., Qavi H., Gaines J. D., Billingsley P., McConnell S. 1985b; Thymidine kinase-negative bovine herpesvirus type 1 mutant is stable and highly attenuated in calves. Archives of Virology 86:63–83
    [Google Scholar]
  18. Kit S., Ichimura H., De Clercq E. 1987; Phosphorylation of nucleoside analogs by equine herpesvirus type 1 pyrimidine deoxyribonucleoside kinase. Antiviral Research 7:53–67
    [Google Scholar]
  19. Kozak M. 1984; Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Research 12:857–872
    [Google Scholar]
  20. Liu Q., Summers W. C. 1988; Site-directed mutagenesis of a nucleotide-binding domain in HSV-1 thymidine kinase: effects on catalytic activity. Virology 163:638–642
    [Google Scholar]
  21. McGeoch D. J., Dalrymple M. A., Davison A. J., Dolan A., Frame M. C., McNab D., Perry L. J., Scott J. E., Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
    [Google Scholar]
  22. Mittal S. K., Field H. J. 1989; Analysis of the bovine herpesvirus type 1 thymidine kinase (TK) gene from wild-type vims and TK- deficient mutants. Journal of General Virology 70:901–918
    [Google Scholar]
  23. Nicolson L., Cullinane A. A., Onions D. E. 1990; The nucleotide sequence of an equine herpesvirus 4 gene homologue of the herpes simplex virus 1 glycoprotein H gene. Journal of General Virology 71:1793–1800
    [Google Scholar]
  24. Otsuka H., Kit S. 1984; Nucleotide sequence of the marmoset herpesvirus thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide. Virology 135:316–330
    [Google Scholar]
  25. Proudfoot N. J., Brownlee G. G. 1976; 3ʹ Non-coding region sequences in eukaryotic messenger RNA. Nature; London: 2632112–14
    [Google Scholar]
  26. Queen C., Korn L. J. 1984; A comprehensive sequence analysis program for the IBM personal computer. Nucleic Acids Research 12:581–599
    [Google Scholar]
  27. Robertson G. R., Whalley J. M. 1988; Evolution of the herpes thymidine kinase gene: identification and comparison of the equine herpesvirus 1 thymidine kinase gene reveals similarity to a cell- encoded thymidylate kinase. Nucleic Acids Research 16:11303–11317
    [Google Scholar]
  28. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A.: 745463–5467
    [Google Scholar]
  29. Scott S. D., Ross N. L. J., Binns M. M. 1989; Nucleotide and predicted amino acid sequences of the Marek’s disease virus and turkey herpesvirus thymidine kinase genes; comparison with thymidine kinase genes of other herpesviruses. Journal of General Virology 70:3055–3065
    [Google Scholar]
  30. Sheppard M., May J. T. 1989; Location and characterization of the bovine herpesvirus type 2 thymidine kinase gene. Journal of General Virology 70:3067–3071
    [Google Scholar]
  31. Tenser R. B., Hay K. A., Edris W. A. 1989; Latency-associated transcript but not reactivatable virus is present in sensory ganglion neurons after inoculation of thymidine kinase-negative mutants of herpes simplex virus type 1. Journal of Virology 63:2861–2865
    [Google Scholar]
  32. Wagner M. J., Sharp J. A., Summers W. C. 1981; Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1. Proceedings of the National Academy of Sciences U.S.A.: 781441–1445
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-8-1801
Loading
/content/journal/jgv/10.1099/0022-1317-71-8-1801
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error