1887

Abstract

Using an SI mapping assay on RNA from cells infected by the nuclear polyhedrosis virus in the presence and absence of cycloheximide and aphidicolin, we can distinguish three classes of transcripts. First, there are those whose synthesis is blocked by the DNA synthesis inhibitor aphidicolin and which are therefore late transcripts. These include the late transcript of the 39K gene and a late leftward transcript across the I site in the dIII-F region. Second, there are those whose synthesis is not blocked by aphidicolin, but whose accumulation is inhibited by the protein synthesis inhibitor cycloheximide and which are therefore presumably delayed early genes. These include the p26 transcript(s), the early 39K transcript and the a transcript in the dIII-K/Q region. Third, there are those whose accumulation is not affected or is enhanced by cycloheximide. These are not necessarily immediate early transcripts, but their response to cycloheximide is clearly different from that of those in the second class. They include the α, and α transcripts in the dIII-K/Q region and the early leftward transcript across the I site in the dIII-F region.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-9-2195
1990-09-01
2024-05-09
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/9/JV0710092195.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-9-2195&mimeType=html&fmt=ahah

References

  1. Crawford A. M., Miller L. K. 1988; Characterization of an early gene accelerating expression of late genes of the baculoviruses Autographa californica nuclear polyhedrosis virus. Journal of Virology 62:2773–2781
    [Google Scholar]
  2. Friesen P. D., Miller L. K. 1985; Temporal regulation of baculovirus RNA: overlapping early and late transcripts. Virology 54:392–400
    [Google Scholar]
  3. Friesen P. D., Miller L. K. 1987; Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the HindIII-K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 61:2264–2272
    [Google Scholar]
  4. Guarino L. A., Summers M. D. 1986; Functional mapping of arrani-activating gene required for expression of a baculovirus delayed-early gene. Journal of Virology 57:563–571
    [Google Scholar]
  5. Guarino L. A., Summers M. D. 1987; Nucleotide sequence and temporal expression of a baculovirus regulatory gene. Journal of Virology 61:2091–2099
    [Google Scholar]
  6. Hardin S. E., Weaver R. F. 1990; Overlapping divergent transcripts mapping to the HindIII F region of the Autographa californica nuclear polyhedrosis virus. Journal of General Virology 71:225–229
    [Google Scholar]
  7. Huh N. E., Weaver R. F. 1990; Identifying the RNA polymerases that synthesize specific transcripts of the Autographa californica nuclear polyhedrosis virus. Journal of General Virology 71:195–201
    [Google Scholar]
  8. Kelly D. C., Lescott T. 1981; Baculovirus replication: protein synthesis in Spodoptera frugiperda cells infected with Trichoplusia ni nuclear polyhedrosis virus. Microbiologica 4:35–47
    [Google Scholar]
  9. Kuzio J., Rohel D. Z., Curry C. J., Krebs A., Carstens C. B., Faulkner P. 1984; Nucleotide sequence of the p10 polypeptide gene of Autographa californica nuclear polyhedrosis virus. Virology 139:414–418
    [Google Scholar]
  10. Lübert H., Doerfler W. 1984; Transcription of overlapping sets of RNAs from the genome of Autographa californica nuclear polyhedrosis virus: a novel method for mapping RNAs. Journal of Virology 52:255–265
    [Google Scholar]
  11. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Nissen M. S., Friesen P. D. 1989; Molecular analysis of the transcriptional regulatory region of an early baculovirus gene. Journal of Virology 63:493–503
    [Google Scholar]
  13. Oellig C., Happ B., Müller T., Doerfler W. 1987; Overlapping sets of viral RNAs reflect array of polypeptides in the EcoRI-J and -N fragments (map positions 81.2 to 85.0) of the Autographa californica nuclear polyhedrosis virus genome. Journal of Virology 61:3048–3057
    [Google Scholar]
  14. Rankin C. D., Ladin B. F., Weaver R. F. 1986; Physical mapping of temporally regulated, overlapping transcripts in the region of the 10K protein gene in Autographa californica nuclear polyhedrosis virus. Journal of Virology 57:18–27
    [Google Scholar]
  15. Rice W. C., Miller L. K. 1986; Baculovirus transcription in the presence of inhibitors and in non-permissive Drosophila cells. Virus Research 6:155–172
    [Google Scholar]
  16. Weaver R. F., Weissman C. 1979; Mapping of an RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15S β-globin mRNA precursor and mature β-globin mRNA have identical map coordinates. Nucleic Acids Research 17:1175–1193
    [Google Scholar]
  17. Vlak J. M., Smith G. E., Summers M. D. 1981; Hybridization selection and in vitro translation of Autographa californica nuclear polyhedrosis virus mRNA. Journal of Virology 40:762–771
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-9-2195
Loading
/content/journal/jgv/10.1099/0022-1317-71-9-2195
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error