1887

Journal of General Virology header logo

Volume 72, Issue 1

Analysis of the Newly Identified Neutralization Epitopes on VP7 of Human Rotavirus Serotype 1 Free

Abstract

Neutralizing monoclonal antibodies (MAbs) directed to the VP7 protein and neutralization-resistant mutants were used to analyse the antigenic structure of VP7 of human rotavirus serotype 1. Cross-neutralization tests using the MAbs and the resistant mutants indicated the existence of two functionally independent neutralization epitope regions (S1 and S2) on VP7. Region S1 corresponds to a single epitope domain of VP7 which has been detected previously. Two MAbs prepared in this study recognized the S1 region, and the resistant mutants they selected had amino acid substitutions at positions 94 or 213. On the other hand, region S2 is considered to be a novel epitope. Single or double amino acid substitutions were detected in the variable regions (amino acid positions 145, 217 and 221) and in the constant regions (positions 104, 201 and 291) of the VP7 protein of mutants selected by MAbs directed to the S2 region. It was suggested that the variable region E (amino acids 208 to 221) includes two independent neutralization sites, and that amino acid substitutions in the constant region of VP7 also affect serotype-specific neutralization epitopes. Neutralization epitopes on VP7 are considered to be highly dependent on the conformation of VP7.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1991
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-1-117
1991-01-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/1/JV0720010117.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-1-117&mimeType=html&fmt=ahah

References

  1. Ahmed M. U., Taniguchi K., Kobayashi N., Urasawa T., Wakasugi F., Islam M., Shaikh H., Urasawa S. 1989; Characterization by enzyme-linked immunosorbent assay using subgroup- and serotype-specific monoclonal antibodies of human rotavirus obtained from diarrheic patients in Bangladesh. Journal of Clinical Microbiology 27:1678–1681
     [Google Scholar]
  2. Baxt B., Vakharia V., Moore D. M., Franke A. J., Morgan D. O. 1989; Analysis of neutralizing antigenic sites on the surface of type A12 foot-and-mouth disease virus. Journal of Virology 63:2143–2151
     [Google Scholar]
  3. Bellinzoni R. B., Mattion N. M., Matson D. O., Blackhall J., La Torre J. L., Scodeller E. A., Urasawa S., Taniguchi K., Estes M.K. 1990; Porcine rotaviruses antigenically related to human rotavirus serotypes 1 and 2. Journal of Clinical Microbiology 28:633–636
     [Google Scholar]
  4. Caton A. J., Brownlee G. G., Yewdell J. W., Gerhard W. 1982; The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype). Cell 31:417–427
     [Google Scholar]
  5. Coelingh K. V. W., Tierney E. L. 1989; Identification of amino acids recognized by syncytium-inhibiting and neutralizing monoclonal antibodies to the human parainfluenza type 3 virus fusion protein. Journal of Virology 63:3755–3760
     [Google Scholar]
  6. Coulson B. S. 1987; Variation in neutralization epitopes of human rotaviruses in relation to genetic RNA polymorphism. Virology 159:209–216
     [Google Scholar]
  7. Coulson B.S., Fowler K.J., Bishop R.F., Cotton R. G. H. 1985; Neutralizing monoclonal antibodies to human rotavirus and indication of antigenic drift among strains from neonates. Journal of Virology 54:14–20
     [Google Scholar]
  8. Dyall-Smith M. L., Lazdins I., Tregear G. W., Holmes I. H. 1986; Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proceedings of the National Academy of Sciences, U.S.A 83:3465–3468
     [Google Scholar]
  9. Flores J., Myslinski J., Kalica A. R., Greenberg H. B., Wyatt R. G., Kapikian A. Z., CHANOCK R. M. 1982; In vitro transcription of two human rotaviruses. Journal of Virology 43:1032–1037
     [Google Scholar]
  10. Georges-Courbot M. C., Beraud A. M., Beards G. M., Campbell A. D., Gonzalez J. P., Georges A.J., Flewett T. H. 1988; Subgroups, serotypes, and electropherotypes of rotavirus isolated from children in Bangui, Central African Republic. Journal of Clinical Microbiology 26:668–671
     [Google Scholar]
  11. Gerhard W., Yewdell J., Frankel M. E. 1981; Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies. Nature, London 290:713–717
     [Google Scholar]
  12. Gerna G., Sarasini A., Matteo A. D., Parea M., Orsolini P., Battaglia M. 1988; Identification of two subtypes of serotype 4 human rotavirus by using VP7-specific neutralizing monoclonal antibodies. Journal of Clinical Microbiology 26:1388–1392
     [Google Scholar]
  13. Glass R. I., Keith J., Nakagomi O., Nakagomi T., Askaa J., Kapikian A. Z., Chanock R. M., Flores J. 1985; Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhoea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology 141:292–298
     [Google Scholar]
  14. Gorziglia M., Hoshino Y., Buckler-White A., Blumentals I., Glass R., Flores J., Kapikian A. Z., Chanock R. M. 1986; Conservation of amino acid sequence of VP8 and cleavage region of 84kDa outer capsid protein among rotaviruses recovered from asymptomatic neonatal infection. Proceedings of the National Academy of Sciences, U.S.A 83:7039–7043
     [Google Scholar]
  15. Green K. Y., Midthun K., Gorziglia M., Hoshino Y., Kapikian A. Z., Chanock R. M., Flores J. 1987; Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology 161:153–159
     [Google Scholar]
  16. Green K. Y., Sears J. F., Taniguchi K., Midthun K., Hoshino Y., Gorziglia M., Nishikawa K., Urasawa S., Kapikian A. Z., Chanock R. M., Flores J. 1988; Prediction of human rotavirus serotype by nucleotide sequence analysis of the VP7 protein gene. Journal of Virology 62:1819–1823
     [Google Scholar]
  17. Green K. Y., Hoshino Y., Ikegami N. 1989; Sequence analysis of the gene encoding the serotype-specific glycoprotein (VP7) of two new human rotavirus serotypes. Virology 168:429–433
     [Google Scholar]
  18. Greenberg H. B., Flores J., Kalica A. R., Wyatt R. G., Jones R. 1983a; Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS–1 strains of human rotavirus. Journal of General Virology 64:313–320
     [Google Scholar]
  19. Greenberg H. B., Valdesuso J., Van Wyke K., Midthun K., Walsh M., McAuliffe V., Wyatt R. G., Kalica A. R., Flores J., Hoshino Y. 1983b; Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. Journal of Virology 47:267–275
     [Google Scholar]
  20. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. 1981; Genes of human (strain Wa) and bovine (strain UK) rotavirus that code for neutralization and subgroup antigens. Virology 112:385–390
     [Google Scholar]
  21. Kalica A. R., Flores J., Greenberg H. B. 1983; Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation. Virology 125:194–205
     [Google Scholar]
  22. Kapikian A. Z., Chanock R. M. 1990; Rotaviruses. In Virology 2nd edn. pp. 1353–1404 Edited by Fields B. N., Knipe D. M. Raven Press; New York:
     [Google Scholar]
  23. Lazdins I., Sonza S., Dyall-Smith M. L., Coulson B. S., Holmes I. H. 1985; Demonstration of an immunodominant neutralization site by analysis of antigenic variants of SA11 rotavirus. Journal of Virology 56:317–319
     [Google Scholar]
  24. Lobigs M., Dalgarno L., Schlesinger J. J., Weir R. C. 1987; Location of a neutralization determinant in the E protein of yellow fever virus (17D vaccine strain). Virology 161:474–478
     [Google Scholar]
  25. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Benfield D. A., Greenberg H. B. 1988a; Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus. Virology 165:511–517
     [Google Scholar]
  26. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Dang M.-N., Greenberg H. B. 1988b; The rhesus rotavirus gene encoding protein VP3: Location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region. Proceedings of the National Academy of Sciences, U.S.A 85:645–649
     [Google Scholar]
  27. Mason B. B., Graham D. Y., Estes M. K. 1983; Biochemical mapping of the simian rotavirus SA-11 genome. Journal of Virology 46:413–423
     [Google Scholar]
  28. Morita Y., Taniguchi K., Urasawa T., Urasawa S. 1988; Analysis of serotype-specific neutralization epitopes on VP7 of human rotavirus by the use of neutralizing monoclonal antibodies and antigenic variants. Journal of General Virology 69:451–458
     [Google Scholar]
  29. Nishikawa K., Hoshino Y., Taniguchi K., Green K. Y., Greenberg H. B., Kapikian A. Z., Chanock R. M., Gorziglia M. 1989; Rotavirus VP7 neutralization epitopes of serotype 3 strains. Virology 171:503–515
     [Google Scholar]
  30. Page G. S., Mosser A. G., Hogle J. M., Filman D. J., Rueckert R. R., Chow M. 1988; Three-dimensional structure of poliovirus serotype 1 neutralizing determinants. Journal of Virology 62:1781–1794
     [Google Scholar]
  31. Shaw R. D., Vo P. T., Offit P. A., Coulson B. S., Greenberg H. B. 1986; Antigenic mapping of the surface proteins of rhesus rotavirus. Virology 155:434–451
     [Google Scholar]
  32. Stocker J. W., Forster H. K., Miggiano V., Stahli C., Staiger G., Takacs B., Staehelin T. 1982; Generation of 2 new mouse myeloma cell lines ‘PAI’ and ‘PAI-0’ for hybridoma production. Research Disclosure 217:155–157
     [Google Scholar]
  33. Taniguchi K., Urasawa S., Urasawa T. 1985; Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. Journal of General Virology 66:1045–1053
     [Google Scholar]
  34. Taniguchi K., Morita Y., Urasawa T., Urasawa S. 1987; Cross-reactive neutralization epitopes on VP3 of human rotavirus: analysis with monoclonal antibodies and antigenic variants. Journal of Virology 61:1726–1730
     [Google Scholar]
  35. Taniguchi K., Hoshino Y., Nishikawa K., Green K. Y., Maloy W. L., Morita Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. 1988a; Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. Journal of Virology 62:1870–1874
     [Google Scholar]
  36. Taniguchi K., Maloy W. L., Nishikawa K., Green K. Y., Hoshino Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. 1988b; Identification of cross-reactive and serotype 2-specific neutralization epitopes on VP3 of human rotavirus. Journal of Virology 62:2421–2426
     [Google Scholar]
  37. Underwood P. A., Skehel J. J., Wiley D. C. 1987; Receptorbinding characteristics of monoclonal antibody-selected antigenic variants of influenza virus. Journal of Virology 61:206–208
     [Google Scholar]
  38. Urasawa S., Urasawa T., Taniguchi K., Chiba S. 1984; Serotype determination of human rotavirus isolates and antibody prevalence in pediatric population in Hokkaido, Japan. Archives of Virology 81:1–12
     [Google Scholar]
  39. Urasawa S., Urasawa T., Taniguchi K. 1986; Genetic reassortment between two human rotaviruses having different serotype and subgroup specificities. Journal of General Virology 67:1551–1559
     [Google Scholar]
  40. Urasawa S., Urasawa T., Taniguchi K., Morita Y., Sakurada N., Saeki Y., Morita O., Hasegawa S. 1988; Validity of an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies for serotyping human rotavirus in stool specimens. Microbiology and Immunology 32:699–708
     [Google Scholar]
  41. Urasawa S., Urasawa T., Taniguchi K., Wakasugi F., Kobaya-shi N., Chiba S., Sakurada N., Morita M., Morita O., Tokieda M., Kawamoto H., Minekawa Y., Ohseto M. 1989; Survey of human rotavirus serotypes in different locals in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies. Journal of Infectious Diseases 160:44–51
     [Google Scholar]
  42. Urasawa T., Urasawa S., Taniguchi K. 1981; Sequential passages of human rotavirus in MA-104 cells. Microbiology and Immunology 25:1025–1035
     [Google Scholar]
  43. Urasawa T., Urasawa S., Chiba Y., Taniguchi K., Kobayashi N., Mutanda L. N., Tukei P. M. 1987; Antigenic characterization of rotaviruses isolated in Kenya from 1982 to 1983. Journal of Clinical Microbiology 25:1891–1896
     [Google Scholar]
  44. Vandepol S. B., Lefrancois L., Holland J. J. 1986; Sequences of the major antibody binding epitopes of the Indiana serotype of vesicular stomatitis virus. Virology 148:312–325
     [Google Scholar]
  45. Ward R. L., Knowlton D. R., Schiff G. M., Hoshino Y., Greenberg H. B. 1988; Relative concentration of serum neutralizing antibody to VP3 and VP7 proteins in adults infected with a human rotavirus. Journal of Virology 62:1543–1549
     [Google Scholar]
  46. Wyatt R. G., James H. D. Jr, Pittman A. L., Hoshino Y., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. 1983; Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. Journal of Clinical Microbiology 18:310–317
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-1-117
Loading
Analysis of the Newly Identified Neutralization Epitopes on VP7 of Human Rotavirus Serotype 1
J. Gen. Virol. 72, 117 (1991); https://doi.org/10.1099/0022-1317-72-1-117
/content/journal/jgv/10.1099/0022-1317-72-1-117
/content/journal/jgv/10.1099/0022-1317-72-1-117
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error