1887

Abstract

The post-translational maturation of the fusion protein (F) of human respiratory syncytial virus was investigated. Chemical cross-linking experiments indicated that F forms homotetramers and provided evidence that the intermonomer contacts involve primarily the F subunit. Homooligomerization as measured by sedimentation in sucrose gradients was insensitive to carbonyl cyanide -chlorophenylhydrazone, indicating that it occurs in the endoplasmic reticulum. Cleavage of the F precursor to yield the F and F subunits was blocked by monensin or brefeldin A, indicating that it takes place in distal cisternae of the trans Golgi compartment or in the more distal trans Golgi network. The F precursor was not detected at the cell surface in surface immunoprecipitation experiments, indicating that cleavage is intracellular. The appearance of the cleaved F protein at the cell surface was concurrent with that of the attachment glycoprotein (G); this and other information indicated that the type 2 membrane orientation of G is not obligatorily associated with a reduced transit rate. Examination of F maturation in the presence of tunicamycin provided evidence that its expression at the cell surface depends upon cleavage and not directly upon glycosylation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-12-3095
1991-12-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/12/JV0720123095.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-12-3095&mimeType=html&fmt=ahah

References

  1. Arumugham R. G., Hildreth S. W., Paradiso P. R. 1989a; Interprotein disulfide bonding between F and G glycoproteins of human respiratory syncytial virus. Archives of Virology 105:65–79
    [Google Scholar]
  2. Arumugham R. G., Hildreth S. W., Paradiso P. R. 1989b; Evidence that the fusion protein of respiratory syncytial virus exist as a dimer in its native form. Archives of Virology 106:327–334
    [Google Scholar]
  3. Arumugham R. G., Hildreth S. W., Paradiso P. R. 1989c; Fatty acid acylation of the fusion glycoprotein of human respiratory syncytial virus. Journal of Biological Chemistry 264:10339–10342
    [Google Scholar]
  4. Beeler J. A., Coelingh K. L. 1989; Neutralization epitopes of the F glycoprotein of respiratory syncytial virus: effect of mutation on fusion function. Journal of Virology 63:2941–2950
    [Google Scholar]
  5. Blumberg B., Giorgi C., Roux L., Raju R., Dowling P., Chollet A., Kolakofsky D. 1985; Sequence determination of the Sendai virus HN gene and its comparison to the influenza virus glycoproteins. Cell 4:269–278
    [Google Scholar]
  6. Buckland R., Wild F. 1989; Leucine zipper motif extends. Nature, London 338:547
    [Google Scholar]
  7. Cash P., Pringle C. R., Preston C. M. 1977; The polypeptides of human respiratory syncytial virus: products of cell-free protein synthesis and post-translational modifications. Virology 92:375–384
    [Google Scholar]
  8. Chege N. W., Pfeffer S. R. 1990; Compartmentation of the Golgi complex: brefeldin A distinguished trans-Golgi cisternae from the trans-Golgi network. Journal of Cell Biology 111:893–899
    [Google Scholar]
  9. Collins P. L. 1991; The molecular biology of human respiratory syncytial virus (RSV) of genus Pneumonovirus. In The Paramyxoviruses pp 103–162 Edited by Kingsbury D. W. New York: Plenum Press;
    [Google Scholar]
  10. Collins P. L., Mottet G. 1991; Homooligomerization of the hemagglutinin-neuraminidase glycoprotein of human parainfluenza virus type 3 occurs prior to the acquisition of correct intramolecular disulfide bonds and mature immunoreactivity. Journal of Virology 65:2362–2371
    [Google Scholar]
  11. Copeland C. S., Zimmer K.-P., Wagner K. R., Healey G. A., Mellman I., Helenius A. 1988; Folding, trimerization, and transport are sequential events in the biogenesis of influenza virus hemagglutinin. Cell 53:197–209
    [Google Scholar]
  12. Doms R. W., Russ G., Yewdell J. W. 1989; Brefeldin A redistributes resident and itinerant Golgi proteins to the endoplasmic reticulum. Journal of Cell Biology 109:61–72
    [Google Scholar]
  13. Dunphy W. G., Rothman J. E. 1985; Compartmental organization of the Golgi stack. Cell 43:13–21
    [Google Scholar]
  14. Fernie B. F., Dapolito G., Cote P. J. Jr, Gerin J. L. 1985; Kinetics of synthesis of respiratory syncytial virus glycoproteins. Journal of General Virology 66:1983–1990
    [Google Scholar]
  15. Griffiths G., Simons K. 1986; The trans-Golgi network: sorting at the exit site of the Golgi complex. Science 234:438–443
    [Google Scholar]
  16. Griffiths G., Quinn P., Warren G. 1983; Dissection of the Golgi complex. I. Monensin inhibits the transport of viral membrane proteins from medial to trans Golgi cisternae in baby hamster kidney cells infected with Semliki Forest virus. Journal of Cell Biology 96:835–850
    [Google Scholar]
  17. Gruber C., Levine S. 1985a; Respiratory syncytial virus polypeptides. IV. The oligosaccharides of the glycoproteins. Journal of General Virology 66:417–432
    [Google Scholar]
  18. Gruber C., Levine S. 1985b; Respiratory syncytial virus polypeptides. V. The kinetics of glycoprotein synthesis. Journal of General Virology 66:1241–1247
    [Google Scholar]
  19. Hsu M.-C., Scheid A., Choppin P. W. 1981; Activation of the Sendai virus fusion protein (F) involves a conformational change with exposure of a new hydrophobic region. Journal of Biological Chemistry 256:3557–3563
    [Google Scholar]
  20. Johnson P. R., Collins P. L. 1988; The fusion glycoproteins of human respiratory syncytial virus of subgroups A and B: sequence conservation provides a structural basis for antigenic relatedness. Journal of General Virology 69:2623–2628
    [Google Scholar]
  21. Klausner R. D. 1989; Sorting and traffic in the central vacuolar system. Cell 57:703–706
    [Google Scholar]
  22. Kohama T., Garten W., Klenk H.-D. 1981; Changes in conformation and charge paralleling proteolytic activation of Newcastle disease virus glycoproteins. Virology 111:364–376
    [Google Scholar]
  23. Kornfeld R., Kornfeld S. 1985; Assembly of asparagine linked oligosaccharides. Annual Review of Biochemistry 54:631–63
    [Google Scholar]
  24. Lippincott-Schwartz J., Donaldson J. G., Schweizer A., Berger E. G., Hauri H.-P., Yuan L. C., Klausner R. D. 1990; Microtubule-dependent retrograde transport of proteins into the ER in the presence of brefeldin A suggests an ER recycling pathway. Cell 60:821–836
    [Google Scholar]
  25. McGinnes L. W., Semerjian A., Morrison T. G. 1985; Conformational changes in Newcastle disease virus fusion glycoprotein during intracellular transport. Journal of Virology 56:341–348
    [Google Scholar]
  26. McIntosh K. M., Chanock R. M. 1990; Respiratory syncytial virus. In Virology, 2nd. edn pp 1045–1072 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  27. Morrison T. G., Ward L. 1984; Intracellular processing of the vesicular stomatitis virus glycoprotein and the Newcastle disease virus hemagglutinin-neuraminidase glycoprotein. Virus Research 1:225–239
    [Google Scholar]
  28. Olmsted R. A., Collins P. L. 1989; The 1A protein of respiratory syncytial virus is an integral membrane protein present as multiple, structurally distinct species. Journal of Virology 63:2019–2029
    [Google Scholar]
  29. Rose J. K., Doms R. W. 1988; Regulation of protein export from the endoplasmic reticulum. Annual Review of Cell Biology 4:257–288
    [Google Scholar]
  30. Sechoy O., Philippot J. R., Bienvenue A. 1987; F protein-F protein interaction within the Sendai virus identified by native bonding or chemical cross-linking. Journal of Biological Chemistry 262:11519–11523
    [Google Scholar]
  31. Spriggs M. K., Olmsted R. A., Venkatesan S., Coligan J. E., Collins P. L. 1986; Fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. Virology 152:241–251
    [Google Scholar]
  32. Vidal S., Mottet G., Kolakofsky D., Roux L. 1989; Addition of high-mannose sugars must precede disulfide bond formation for proper folding of Sendai virus glycoproteins. Journal of Virology 63:892–900
    [Google Scholar]
  33. Walsh E. E., Brandriss M. W., Schlesinger J. J. 1985; Purification and characterization of the respiratory syncytial virus fusion protein. Journal of General Virology 66:409–415
    [Google Scholar]
  34. Wertz G. W., Kreiger M., Ball L. A. 1989; Structure and cell surface maturation of the attachment glycoprotein of human respiratory syncytial virus in a cell line deficient in O glycosylation. Journal of Virology 63:4767–4776
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-12-3095
Loading
/content/journal/jgv/10.1099/0022-1317-72-12-3095
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error