1887

Abstract

The range of hepatitis A virus (HAV) particles generated during persistent infection of different cell lines was studied. Buoyant density and sedimentation analyses of cell extracts revealed a uniform profile of particles in all cell lines analysed except for BS-C-1 cells. The virion itself usually represented less than 50% of the total mass of virus antigen. A major portion of the antigen was associated with non-infectious, empty particles, which banded at 1.305 g/ml and 1.20 g/ml CsCl, and sedimented in sucrose gradients at 76S and 59S. Empty HAV particles were similar to those of poliovirus with respect to their physical stability and had the characteristic capsid protein content (VP0, VP1 and VP3). An additional RNA-containing particle, probably the provirion, represented only a minor species characterized by a buoyant density of 1.32 g/ml in CsCl and sedimenting at 130S.

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1991-09-01
2024-04-30
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References

  1. Anderson D. A., Ross B. C. 1990; Morphogenesis of hepatitis A virus: isolation and characterization of subviral particles. Journal of Virology 64:5284–5289
    [Google Scholar]
  2. Andre F. E., Hepburn A., d’Hondt E. 1990; Inactivated candidate vaccines for hepatitis-A. Progress in Medical Virology 37:72–95
    [Google Scholar]
  3. Coulepis A. G., Locarnini A. S., Westaway E. G., Tannock G. A., Gust I. D. 1982; Biophysical and biochemical characterization of hepatitis A virus. Intervirology 18:107–127
    [Google Scholar]
  4. de Chastonay J., Siegl G. 1987; Replicative events in hepatitis A virus-infected MRC-5 cells. Virology 157:268–275
    [Google Scholar]
  5. Fernandez-Thomas C. B., Baltimore D. 1973; Morphogenesis of polio-virus. II. Demonstration of a new intermediate, the provirion. Journal of Virology 12:1122–1130
    [Google Scholar]
  6. Flehmig B. 1981; Hepatitis A virus in cell culture. II. Growth characteristics of hepatitis A virus in Frhk-4/R cells. Medical Microbiology and Immunology 170:73–81
    [Google Scholar]
  7. Flehmig B., Heinricy U., Pfisterer M. 1989; Immunogenicity of a killed hepatitis A vaccine in seronegative volunteers. Lancet i:1039–1041
    [Google Scholar]
  8. Frösner G. G., Overby L. R., Flehmig B., Gerth H. J., Haas H., Decker R. H., Ling C. M., Zuckerman A. J., Frösner H. R. 1977; Seroepidemiological investigation of patients and family contacts in an epidemic of hepatitis A. Journal of Medical Virology 1:163–173
    [Google Scholar]
  9. Gauss-Müller V., Lottspeich F., Deinhardt F. 1986; Characterization of hepatitis A virus structural proteins. Virology 155:732–736
    [Google Scholar]
  10. Gromeier M., Wetz K. 1990; Kinetics of poliovirus in HeLa cells in a non-acidic environment. Journal of Virology 64:3590–3597
    [Google Scholar]
  11. Heinricy U., Stierhof Y. -D., Pfisterer M., Flehmig B. 1987; Properties of a hepatitis A virus candidate vaccine strain. Journal of General Virology 68:2487–2493
    [Google Scholar]
  12. Koch F., Koch G. 1985 The Molecular Biology of Poliovirus Wien & New York: Springer-Verlag;
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Lee P. W. K., Colter J. S. 1979; Further characterization of mengo subviral particles: a new hypothesis for piconavirus assembly. Virology 97:266–274
    [Google Scholar]
  15. Lee P. W. K., Paucha E., Colter J. S. 1978; Identification and partial characterization of a new (50S) subviral particle in Mengo virus-infected L cells. Virology 85:286–295
    [Google Scholar]
  16. Lemon S. M., Binn L. N. 1985; Incomplete neutralization of hepatitis A virus in vitro due to lipid-associated virions. Journal of General Virology 66:2501–2505
    [Google Scholar]
  17. Lemon S. M., Jansen R. W., Newbold J. E. 1985; Infectious hepatitis A virus particles produced in cell culture consist of three distinct types with different buoyant densities in CsCl. Journal of Virology 54:78–85
    [Google Scholar]
  18. Onodera S., Phillips B. A. 1987; A novel method for obtaining poliovirus 14S pentamers from procapsids and their self-assembly into virus-like shells. Virology 159:278–287
    [Google Scholar]
  19. Onodera S., Cardamone J. J., Phillips J. R., Phillips B. A. 1986; Biological activity and electron microscopy of poliovirus 14S particles obtained from alkali-dissociated procapsids. Journal of Virology 58:610–618
    [Google Scholar]
  20. Ping L. H., Jansen R. W., Stapelton J. T., Cohen J. I., Lemon S. L. 1988; Identification of an immunodominant antigenic site involving the capsid protein VP3 of hepatitis A virus. Proceedings of the National Academy of Sciences, U.S.A. 85:8281–8285
    [Google Scholar]
  21. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  22. Siegl G., Frösner G. G. 1978; Characterization and classification of virus particles associated with hepatitis A. I. Size, density and sedimentation. Journal of Virology 26:40–47
    [Google Scholar]
  23. Siegl G., Frösner G. G., Gauss-Muller V., Tratschin J. -D., Deinhardt F. 1981; The physicochemical properties of infectious hepatitis A virions. Journal of General Virology 57:331–341
    [Google Scholar]
  24. Siegl G., de Chastonay J., Kronauer G. 1984a; Propagation and assay of hepatitis A virus in vitro. Journal of Virological Methods 9:53–67
    [Google Scholar]
  25. Siegl G., Weitz M., Kronauer G. 1984b; Stability of hepatitis A virus. Intervirology 22:218–226
    [Google Scholar]
  26. Sjogren M. H., Eckels K. H., Binn L. N., Dubois D. R., Hoke C. H., Burke D. S., Bancroft W. H. 1988; Safety and immunogenicity of an inactivated hepatitis A vaccine. In Viral Hepatitis and Liver Disease pp 94–96 Edited by Zuckerman A. New York: Alan R. Liss;
    [Google Scholar]
  27. Stapelton J. T., Lange D. K. 1988; Trypsin sensitivity of hepatitis A virus. In Vaccines ′88: New Chemical and Genetic Approaches to Vaccination pp 235–239 Edited by Ginsberg J., Brown F., Lerner R. A., Chanock R. M. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Ticehurst J. R., Racaniello V. R., Baroudy B. M., Baltimore D., Purcell R. H., Feinstone S. M. 1983; Molecular cloning and characterization of hepatitis A virus cDNA. Proceedings of the National Academy of Sciences, U.S.A 80:5885–5889
    [Google Scholar]
  29. Weitz M., Finkel-Jimenez B., Siegl G. 1990 Viral Hepatitis and Liver Disease Baltimore: Williams & Wilkins; (in press)
    [Google Scholar]
  30. Wheeler C. M., Fields H. A., Schable C. A., Meinke W. J., Maynard J. E. 1986a; Adsorption, purification, and growth characteristics of hepatitis A virus strain HAS-15 propagated in fetal rhesus monkey kidney cells. Journal of Clinical Microbiology 23:434–440
    [Google Scholar]
  31. Wheeler C. M., Robertson B. H., Van Nest G., Dina D., Bradley D. W., Fields H. A. 1986b; Structure of the hepatitis A virion: peptide mapping of the capsid region. Journal of Virology 58:307–313
    [Google Scholar]
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