1887

Abstract

Chimpanzees were inoculated intravenously with the H strain of hepatitis C virus (HCV), and analysed for viraemia using the polymerase chain reaction and for a humoral immune response using first and second generation anti-HCV ELISAs and an immunoblot assay (4-RIBA). In all seven chimpanzees studied, viraemia occurred several weeks before a significant increase in serum alanine transferase (ALT) activity, whereas the first circulating anti-HCV antibodies became detectable at the time of significant increase in ALT levels, provided the second generation ELISA or 4-RIBA was used. On the basis of the duration of viraemia the chimpanzees studied could be assigned to two different groups: those in which viraemia disappeared in conjunction with or shortly after seroconversion, and those remaining viraemic for many weeks after the appearance of antibodies. The clearance of HCV from the circulation did not correlate with the antibody pattern determined using 4-RIBA, i.e. the HCV-specific assays currently available do not enable us to predict whether an infected chimpanzee will develop persistent viraemia. Only two of the seven chimpanzees analysed developed anti-core protein (c-22) antibodies, which appeared at the same time as the first ALT peak, whereas all animals developed antibodies to the non-structural protein, c-33, and these antibodies persisted.

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1992-04-01
2024-04-26
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References

  1. Chiba J., Ohba H., Matsuura Y., Watanabe Y., Katayama T., Kikuchi S., Saito I., Miyamura T. 1991; Serodiagnosis of hepatitis C virus (HCV) with an HCV core protein molecularly expressed by a recombinant baculovirus. Proceedings of the National Academy of Sciences, U.S.A 88:4641–4645
    [Google Scholar]
  2. Choo Q. L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. 1989; Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359–362
    [Google Scholar]
  3. Esteban J. J., Gonzalez A., Hernandez J. M., Viladomiu L., Sanchez C., Lopez-Talavera J. C., Lucea D., Martin-Vega C., Vidal X., Esteban R., Guardia J. 1990; Evaluation of antibodies to hepatitis C virus in a study of transfusion-associated hepatitis. New England Journal of Medicine 323:1107–1112
    [Google Scholar]
  4. Farci P., Alter H. J., Wong D., Miller R. H., Shih J. W., Jett B., Purcell R. H. 1991; A long-term study of hepatitis C virus replication in non-A, non-B hepatitis. New England Journal of Medicine 325:98–104
    [Google Scholar]
  5. Feinstone S. M., Alter H. J., Dienes H. P., Shimizu Y. K., Popper H., Blackmore D., Sly D., London W. T., Purcell R. H. 1981; NonA/nonB hepatitis in chimpanzees and marmosets. Journal of Infectious Diseases 144:588–598
    [Google Scholar]
  6. Fuchs K., Motz M., Schreier E., Zachoval R., Deinhardt F., Roggendorf M. 1991; Characterization of nucleotide sequences from European hepatitis C virus isolates. Gene 103:163–169
    [Google Scholar]
  7. Garson J. A., Tedder R. S., Briggs M., Tuke P., Glazebrook J. A., Trute A., Parker D., Barbara J. A. J., Conteras M., Aloysius S. 1990a; Detection of hepatitis C viral sequences in blood donation by ‘nested’ polymerase chain reaction and prediction of infectivity. Lancet i:1419–1422
    [Google Scholar]
  8. Garson J. A., Tuke P. W., Makris M., Briggs M., Machin S. J., Preston F. E., Tedder R. S. 1990b; Demonstration of viraemia patterns in haemophiliacs treated with hepatitis-C-virus-contami-nated factor VIII concentrates. Lancet ii:1022–1025
    [Google Scholar]
  9. Harada S., Watanabe Y., Takeuchi K., Suzuki T., Katayama T., Takebe Y., Saito I., Miyamura T. 1991; Expression of processed core protein of hepatitis C virus in mammalian cells. Journal of Virology 65:3015–3021
    [Google Scholar]
  10. Mauler R., Merkle W., Hilfenhaus J. 1987; Inactivation of HTLV-III/LAV, hepatitis B and nonA/nonB hepatitis viruses by pasteurization in human plasma preparations. Developments in Biological Standardization 67:337–351
    [Google Scholar]
  11. Nasoff M. S., Zehedee S. L., Inchauspe G., Prince A. M. 1991; Identification of an immunodominant epitope within the capsid protein of hepatitis C virus. Proceedings of the National Academy of Sciences, U.S.A 88:5462–5466
    [Google Scholar]
  12. Ogata N., Alter H. J., Miller R. H., Purcell R. H. 1991; Nucleotide sequence and mutation rate of the H strain of hepatitis C virus. Proceedings of the National Academy of Sciences, U.S.A 88:3392–3396
    [Google Scholar]
  13. Okamoto H., Okada S., Sugiyama Y., Yotsumoto S., Tanaka T., Yoshizawa H., Tsuda F., Miyakawa Y., Mayumi M. 1990; The 5′-terminal sequence of hepatitis C virus genome. Japanese Journal of Experimental Medicine 60:167–177
    [Google Scholar]
  14. Prince A., Stephan W., Brotman B. 1983; Betapropiolactone ultraviolet irradiation: a review of its effectiveness against viruses in blood derivatives. Reviews of Infectious Diseases 5:92–107
    [Google Scholar]
  15. Shimizu Y. K., Weiner A. J., Rosenblatt J., Wong D. C., Shapiro M., Popkin T., Houghton M., Alter H. J., Purcell R. H. 1990; Early events in hepatitis C virus infection of chimpanzees. Proceedings of the National Academy of Sciences, U.S.A 87:6441–6444
    [Google Scholar]
  16. van der Poel C. L., Cuypers H. T. M., Reesink H. W., Weiner A. J., Quan S., Di Nello R., van Boven J. J. P., Winkel I., Mulder-Folkerts D., Exel-Oehlers P. J., Schaasberg W., Leentvaar-Kuypers A., Polito A., Houghton M., Lelie P. 1991; Confirmation of hepatitis C virus infection by new four-antigen recombinant immunoblot assay. Lancet i:317–319
    [Google Scholar]
  17. Weiner A. J., Kuo G., Bradley D. W., Bonino F., Saracco G., Lee C., Rosenblatt I., Choo Q. L., Houghton M. 1990; Detection of hepatitis C viral sequences in nonA/nonB hepatitis. Lancet ii:1–3
    [Google Scholar]
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