1887

Journal of General Virology header logo

Volume 73, Issue 7

Failure to isolate human T cell lymphotropic virus type I and to detect variant-specific genomic sequences by polymerase chain reaction in Melanesians with indeterminate Western immunoblot Free

Abstract

The controversy over the endemicity of human T cell lymphotropic virus type I (HTLV-I) in Melanesia has been settled recently by the isolation of genetically distinct, highly divergent sequence variants of HTLV-I from unrelated inhabitants of Papua New Guinea and the Solomon Islands. Still at issue, however, is the significance of the high frequency of indeterminate HTLV-I Western blots (defined as reactivity to only -encoded proteins) among Melanesians. To investigate whether this indeterminate seroreactivity reflects specific reactivity to the Melanesian HTLV-I variants, 27 seroindeterminate Melanesians from Papua New Guinea and the Solomon Islands were studied for evidence of HTLV-I infection. Although antibodies against Melanesian variant-specific gene products and variant-specific gene sequences were detected by Western blot analysis and polymerase chain reaction, respectively, in all 11 HTLV-I Western blot-positive Melanesians, none of the 27 seroindeterminate Melanesians had such variant-specific antibodies or HTLV-I proviral sequences. In addition, attempts to isolate HTLV-I from seroindeterminate individuals were unsuccessful. These data indicate that HTLV-I infection is not the cause of the indeterminate Western blot reactivity seen in Melanesia.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1992
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-7-1805
1992-07-01
2024-05-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/7/JV0730071805.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-7-1805&mimeType=html&fmt=ahah

References

  1. Armstrong M. Y. K., Hrdy D. B., Carlson J. R., Friedlaender J. S. 1990; Prevalence of antibodies interactive with HTLV-I antigens in selected Solomon Islands populations. American Journal of Physical Anthropology 81:465–470
     [Google Scholar]
  2. Asher D. M., Goudsmit J., Pomeroy K. L., Garruto R. M., Bakker M., Ono S. G., Elliott N., Harris K., Askins H., Eldadah Z., Goldstein A. D., Gajdusek D. C. 1988; Antibodies to HTLV-I in populations of the southwestern Pacific. Journal of Medical Virology 26:339–351
     [Google Scholar]
  3. Behets F., Disasi A., Ryder R. W., Bishagara K., Piot P., Kashamuka M., Kamenga M., Nzila N., Laga M., Vercau-teren G., Batter V., Brown C., Quinn T. 1991; Comparison of five commercial enzyme-linked immunosorbent assays and Western immunoblotting for human-immunodeficiency virus antibody detection in serum samples from central Africa. Journal of Clinical Microbiology 29:2280–2284
     [Google Scholar]
  4. Constantine N., Fox E. 1989; Need to confirm HTLV-I screening assays. Lancet i:108–109
     [Google Scholar]
  5. De B. K., Srinivasan A. 1989; Multiple primers for the detection of HTLV-I by PCR. Nucleic Acids Research 17:2142
     [Google Scholar]
  6. Delaporte E., Dupont A., Merlin M., Josse R., Hamono B., Larouze B., Brun-Vezinet F. 1988; Seroepidemiological survey of HIV-1 and HIV-2 antibodies in Gabon. AIDS 2:136–137
     [Google Scholar]
  7. Delaporte E., Peeters M., Simoni M., Piot P. 1989; HTLV-I infection in western equatorial Africa. Lancet ii 1226
     [Google Scholar]
  8. Delaporte E., Monplaisir N., Louwagie J., Peeters M., Martin-Prevel Y., Louis J.-P., Trebuco A., Bedjabaga L., Ossari S., Honore C., Larouze B., D’Auriol L., van der Groen G., Pliot P. 1991; Prevalence of HTLV-I and HTLV-II infection in Gabon, Africa: comparison of the serological and PCR results. International Journal of Cancer 49:373–376
     [Google Scholar]
  9. Dock N. L., Kleinman S. H., Rayfield M. A., Schabel C. A., Williams A. E., Dodd R. Y. 1991; Human immunodeficiency virus infection and indeterminate Western blot patterns. Archives of Internal Medicine 151:525–530
     [Google Scholar]
  10. Garruto R. M., Yanagihara R., Asher D. M. 1989; Seroepidemiology of human T-lymphotropic virus type I in the Pacific region. In Retrovirus Humanos HTLV-I Paraparesia Espas-tica y Linfomas pp 95–108 Edited by Zaninovic V. Cali: Editorial Feriva;
     [Google Scholar]
  11. Garruto R. M., Slover M., Yanagihara R., Mora C. A., Alexander S. S., Asher D. M., Rodgers-Johnson P., Gajdusek D. C. 1990; High prevalence of human T-lymphotropic virus type I infection in isolated populations of the Western Pacific confirmed by Western immunoblot. American Journal of Human Biology 2:439–447
     [Google Scholar]
  12. Gessain A., Barin F., Vernant J. C., Gout O., Calender A., De The G. 1985; Antibodies to human T lymphotropic virus type I in patients with tropical spastic paraparesis. Lancet ii:407–410
     [Google Scholar]
  13. Gessain A., Yanagihara R., Franchini G., Garruto R. M., Jenkins C. L., Ajdukiewicz A. B., Gallo R. C., Gajdusek D. C. 1991; Highly divergent molecular variants of human T-lymphotropic virus type I from isolated populations in Papua New Guinea and the Solomon Islands. Proceedings of the National Academy of Sciences, U.S.A. 88:7694–7698
     [Google Scholar]
  14. Hayes C. G., Burans J. P., Oberst R. B. 1991; Antibodies to human T lymphotropic virus type I in a population from the Philippines: evidence for cross-reactivity with Plasmodium falciparum. Journal of Infectious Diseases 163:257–262
     [Google Scholar]
  15. Hinuma Y., Nagata K., Hanaoka M., Nakai M., Matsumoto T., Kinoshita K., Shirakawa S., Miyoshi I. 1981; Adult T-cell leukemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. Proceedings of the National Academy of Sciences, U.S.A. 78:6476–6480
     [Google Scholar]
  16. Huet T., Dazza M.-C., Brun-Vezinet F., Roelants G. E., Wain-Hobson S. 1989; A highly defective HIV-1 strain isolated from a healthy Gabonese individual presenting an atypical Western blot. AIDS 3:707–715
     [Google Scholar]
  17. Imagawa D. T., Lee M. H., Wolinsky S. M., Sano K., Morales F., Kwok S., Sninsky J. J., Nishanian P. G., Giorgi J., Fahey J. L., Dudley J., Visscher B. R., Detels R. 1989; Human immunodeficiency virus type 1 infection in homosexual men who remain seronegative for prolonged periods. New England Journal of Medicine 320:1458–1462
     [Google Scholar]
  18. Imai J., Terashi S., Talonu T., Komoda H., Taufa T., Nurse G. T., Babona D., Yamaguchi K., Nakashima H., Ishikawa K., Kawamura M., Hayami M. 1990; Geographical distribution of subjects seropositive for human T-cell leukemia virus type I in Papua New Guinea. Japanese Journal of Cancer Research 81:1218–1221
     [Google Scholar]
  19. Jackson J. B., MacDonald K. L., Cadwell J., Sullivan C., Kline W. E., Hanson M., Sannerud K. J., Stramer S. L., Fildes N. J., Kwok S. Y., Sninsky J. J., Bowman R. J., Polesky F. F., Balfour H. H. Jr, Osterholm M. T. 1990; Absence of HIV infection in blood donors with indeterminate Western blot tests for antibody to HIV-1. New England Journal of Medicine 322:217–222
     [Google Scholar]
  20. Jenkins C., Dimitrakakis M., Cook I., Sanders R., Stallman N. 1989; Culture change and epidemiological patterns among the Hagahai, Papua New Guinea. Human Ecology 17:27–57
     [Google Scholar]
  21. Jennings G. B., Bangs M. J., Sie A., Anthony R. L. 1991; Prevalence of serum antibodies to HTLV-I in an isolated community in the highlands of Irian Jaya, Indonesia. Abstracts, 40th Annual Meeting of the American Society of Tropical Medicine and Hygiene, Boston
     [Google Scholar]
  22. Kazura J. W., Saxinger W. C., Wenger J., Forsyth K., Lederman M. M., Gillespie J. A., Carpenter C. C. J., Alpers M. P. 1987; Epidemiology of human T-cell leukemia virus type I infection in East Sepik Province, Papua New Guinea. Journal of Infectious Diseases 155:1100–1107
     [Google Scholar]
  23. Kitajima I., Maruyama I., Maruyama Y., Ijichi S., Eiraku N., Mimura Y., Osame M. 1989; Polyarthritis in human T lymphotropic virus type I-associated myelopathy. Arthritis and Rheumatism 32:1342–1344
     [Google Scholar]
  24. Kwok S., Kellogg D., Ehrlich G., Poiesz B., Bhagavati S., Sninsky J. J. 1988; Characterization of a sequence of human T cell leukemia virus type I from a patient with chronic progressive myelopathy. Journal of Infectious Diseases 158:1193–1197
     [Google Scholar]
  25. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  26. LaGrenade L., Hanchard B., Fletcher V., Cranston B., Blattner W. 1990; Infective dermatitis of Jamaican children: a marker for HTLV-I infection. Lancet 336:1345–1347
     [Google Scholar]
  27. Lal R. B., Sulzer A., Shi Y. P., Sinha S., Alpers M., Povoa M., Roberts C., Lal A. A. 1991; >Lack of cross-reactivity between antibodies to malarial antigens and human T-lymphotropic virus type I/II. Abstracts, 40th Annual Meeting of the American Society of Tropical Medicine and Hygiene, Boston
     [Google Scholar]
  28. Lee H., Swanson P., Shorty V. S., Zack J. A., Rosenblatt J. D., Chen I. S. Y. 1989; High rate of HTLV-II infection in seropositive IV drug abusers in New Orleans. Science 244:471–475
     [Google Scholar]
  29. Miyoshi I., Kubonishi L., Yoshimoto S., Akagi T., Ohtuski Y., Shiraishi Y., Nagata K., Hinuma Y. 1981; Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T-cells. Nature, London 294:770–771
     [Google Scholar]
  30. Morgan O. St C., Mora C., Rodgers-Johnson P., Char G. 1989; HTLV-I and polymyositis in Jamaica. Lancet ii:1184–1187
     [Google Scholar]
  31. Nerurkar V. R., Yanagihara R. 1992; Specificity of an oligonucleotide primer pair and of a single-base substitution in the amplification and detection of env gene sequences of HTLV-I variants from Papua New Guinea and the Solomon Islands. AIDS Research and Human Retroviruses (in press)
    [Google Scholar]
  32. Osame M., Usuku M., Izumo S., Ijichi N., Amitani H., Igata A., Matsumoto M., Tara M. 1986; HTLV-I-associated myelopathy, a new clinical entity. Lancet i:1031–1032
     [Google Scholar]
  33. Ouattara S. A., Chotard J., Meite M., Selly-Essis A. M., de The G. 1986; Retrovirus infection (LAV/HTLV-III and HTLV-I) in Ivory Coast, West Africa. Annales de l’lnstitut Pasteur 137E:303–310
     [Google Scholar]
  34. Reddy E. P., Andberg-Wollheim M., Mettus R. V., Ray P. E., Defreitas E., Koprowski H. 1989; Amplification and molecular cloning of HTLV-I sequences from DNA of multiple sclerosis patients. Science 243:529–533
     [Google Scholar]
  35. Rodgers-Johnson P., Gajdusek D. C., Morgan O. St C., Zaninovic V., Sarin P. S., Graham D. S. 1985; HTLV-I and HTLV-III antibodies and tropical spastic paraparesis. Lancet ii:1247–1248
     [Google Scholar]
  36. Sanders R. C., Levin A., Anian G., Webber I., Lee H., Swanson P., Diwan A., Desowitz R., Blattner W. A., Alpers M. P. 1990; HTLV-I antibody studies in villagers in East Sepik Province, Papua New Guinea. Archives of Virology 114:27–35
     [Google Scholar]
  37. Sherman M. P., Saksena N. K., Dube D. K., Yanagihara R., Poiesz B. J. 1992; Evolutionary insights on the origin of HTLV-I derived from sequence analysis of a new HTLV-I variant from Papua New Guinea. Journal of Virology 66:2556–2563
     [Google Scholar]
  38. Takatsuki K., Uchiyama J., Sagawa K., Yodoi J. 1977; Adult T-cell leukemia in Japan. In Topics in Hematology pp 73–77 Edited by Seno S., Takaku K., Irino S. Amsterdam: Excerpta Medica;
     [Google Scholar]
  39. Weber J. N., Banatvala N., Clayden S., McAdam K. P. W. J., Palmer S., Moulsdale H., Tosswill J., Dilger P., Thorpe R., Amann S. 1989; HTLV-I infection in Papua New Guinea: evidence for serologic false positivity. Journal of Infectious Diseases 159:1025–1028
     [Google Scholar]
  40. Yanagihara R., Jenkins C. L., Alexander S. S., Mora C. A., Garruto R. M. 1990; Human T lymphotropic virus type I infection in Papua New Guinea: high prevalence among the Hagahai confirmed by Western analysis. Journal of Infectious Diseases 162:649–654
     [Google Scholar]
  41. Yanagihara R., Ajdukiewicz A. B., Garruto R. M., Sharlow E. R., Wu X. Y., Alemaena O., Sale H., Alexander S. S., Gajdusek D. C. 1991a; Human T lymphotropic virus type I infection in the Solomon Islands. American Journal of Tropical Medicine and Hygiene 44:122–130
     [Google Scholar]
  42. Yanagihara R., Ajdukiewicz A. B., Nerurkar V. R., Garruto R. M., Gajdusek D. C. 1991b; Verification of HTLV-I infection in the Solomon Islands by virus isolation and gene amplification. Japanese Journal of Cancer Research 82:240–244
     [Google Scholar]
  43. Yanagihara R., Jenkins C. L., Ajdukiewicz A. B., Lal R. B. 1991c; Serological discrimination of HTLV I and II infection in Melanesia. Lancet 337:617–618
     [Google Scholar]
  44. Yanagihara R., Nerurkar V. R., Ajdukiewicz A. B. 1991d; Comparisons between strains of human T lymphotropic virus type I isolated from inhabitants of the Solomon Islands and Papua New Guinea. Journal of Infectious Diseases 164:443–449
     [Google Scholar]
  45. Yanagihara R., Nerurkar V. R., Garruto R. M., Miller M. A., Leon-Monzon M. E., Jenkins C. L., Sanders R. C., Liberski P. P., Alpers M. P., Gajdusek D. C. 1991e; Characterization of a variant of human T lymphotropic virus type I isolated from a member of a remote, recently contacted group in Papua New Guinea. Proceedings of the National Academy of Sciences, U.S.A. 88:1446–1450
     [Google Scholar]
  46. Yoshida M., Miyoshi I., Hinuma Y. 1982; Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implication in the disease. Proceedings of the National Academy of Sciences, U.S.A. 792031–2035
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-7-1805
Loading
Failure to isolate human T cell lymphotropic virus type I and to detect variant-specific genomic sequences by polymerase chain reaction in Melanesians with indeterminate Western immunoblot
J. Gen. Virol. 73, 1805 (1992); https://doi.org/10.1099/0022-1317-73-7-1805
/content/journal/jgv/10.1099/0022-1317-73-7-1805
/content/journal/jgv/10.1099/0022-1317-73-7-1805
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error