1887

Abstract

The N-terminal P1 protein of turnip mosaic potyvirus (TuMY) polyprotein was overexpressed in , purified by metal chelation chromatography under denaturing conditions and renatured. U.v. cross-linking experiments indicated that the recombinant protein interacted with RNA, and gel retardation electrophoresis demonstrated that more than one molecule of PI bound one molecule of RNA. Formation of the protein-RNA complexes was dependent on the conformational state of PI and was stable at relatively high concentrations of NaCl. P1 had the ability to bind ssRNA and ssDNA, with similar affinity, but was not able to bind to dsDNA. The TuMV protein had the additional characteristic of binding dsRNA with affinity similar to that observed with single-stranded nucleic acids.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-75-10-2567
1994-10-01
2024-05-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/75/10/JV0750102567.html?itemId=/content/journal/jgv/10.1099/0022-1317-75-10-2567&mimeType=html&fmt=ahah

References

  1. Atreya C. D., Raccah B., Pirone T. P. 1990; A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178:161–165
    [Google Scholar]
  2. Brantley J. D., Hunt A. G. 1993; The N-terminal protein of the polyprotein encoded by the potyvirus tobacco vein mottling virus is an RNA-binding protein. Journal of General Virology 74:1157–1162
    [Google Scholar]
  3. Calnan B. J., Tidor B., Biancalana S., Hudson D., Frankel A. D. 1991; Arginine-mediated RNA recognition: the arginine fork. Science 252:1167–1171
    [Google Scholar]
  4. Carrington J. C., Dougherty W. G. 1987; Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease. Virology 61:2540–2548
    [Google Scholar]
  5. Carrington J. C., Herndon K. L. 1992; Characterization of the potyviral HC-Pro autocatalytic cleavage site. Virology 187:308–315
    [Google Scholar]
  6. Citovsky V., Wong M. L., Zambryski P. 1989; Cooperative interaction of Agrobacterium VirE2 protein with single-stranded DNA: implications for the T-DNA transfer process. Proceedings of the National Academy of Sciences U.S.A.: 861193–1197
    [Google Scholar]
  7. Citovsky V., Knorr D., Schuster G., Zambryski P. 1990; The P30 movement protein of tobacco mosaic virus is a single-strand nucleic acid binding protein. Cell 60:673–647
    [Google Scholar]
  8. Citovsky V., Knorr D., Zambryski P. 1991; Gene 1, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA binding protein. Proceedings of the National Academy of Sciences U.S.A.: 883476–3480
    [Google Scholar]
  9. Citovsky V., Wong M. E., Shaw A. L., Venkataram P., Zambryski P. 1992; Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids. Plant Cell 4:397–111
    [Google Scholar]
  10. Domier L. L., Shaw J. G., Rhoads R. E. 1987; Poty virus proteins share amino acid sequence homology with picorna-, como-, and caulimoviral proteins. Virology 178:285–288
    [Google Scholar]
  11. Dougherty W. G., Carrington J. C. 1988; Expression and function of potyviral gene products. Annual Review of Phytopathology 26:123–142
    [Google Scholar]
  12. Dougherty W. G., Parks T. D. 1991; Post-translational processing of the tobacco etch virus 49-kDa small nuclear inclusion polyprotein: identification of an internal cleavage site and delimitation of VPg and proteinase domains. Virology 183:449–456
    [Google Scholar]
  13. Garcia J. A., Riechmann J. L., Laín S. 1989; Proteolytic activity of the plum pox potyvirus NIa-like protein in Escherichia coli . Virology 170:362–369
    [Google Scholar]
  14. Giesman-Cookmeyer D., Lommel S. A. 1993; Alanine scanning mutagenesis of a plant virus movement protein identifies three functional domains. Plant Cell 5:973–982
    [Google Scholar]
  15. Hellmann G. M., Shaw J. G., Rhoads R. E. 1988; In vitro analysis of tobacco vein mottling virus NIa cistron: evidence for a virus-encoded protease. Virology 163:554–562
    [Google Scholar]
  16. Heppell J., Berthiaume L., Tarrab E., Lecomte J., Arella M. 1992; Evidence of genomic variations between infectious pancreatic necrosis virus strains determined by restriction fragment profiles. Journal of General Virology 73:2863–2870
    [Google Scholar]
  17. Koonin E. V., Mushegian A. R., Ryabov E. V., Dolja V. V. 1991; Diverse groups of plant RNA and DNA viruses share related movement proteins that may possess chaperone-like activity. Journal of General Virology 72:2895–2903
    [Google Scholar]
  18. Laín S., Riechmann J. L., García J. A. 1990; RNA helicase: a novel activity associated with a protein encoded by a positive strand RNA virus. Nucleic Acids Research 18:7003–7006
    [Google Scholar]
  19. Laín S., Martin M. T., Riechmann J. L., García J. A. 1991; Novel catalytic activity associated with positive-strand RNA virus infection: nucleic acid-stimulated ATPase activity of the plum pox potyvirus helicase-like protein. Journal of Virology 63:1–6
    [Google Scholar]
  20. Laliberté J.-F., Nicolas O., Chatel H., Lazure C., Morosoli R. 1992; Release of a 22-kDa protein derived from the amino- terminal domain of the 49-kDa NIa of turnip mosaic potyvirus in Escherichia coli . Virology 190:510–514
    [Google Scholar]
  21. Lazinski D., Grzadzielska E., Das A. 1989; Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators requires a conserved arginine-rich motif. Cell 59:207–218
    [Google Scholar]
  22. Melcher U. 1990; Similarities between putative transport proteins of plant viruses. Journal of General Virology 71:1009–1018
    [Google Scholar]
  23. Murphy J. F., Rhoads R. E., Hunt A. G., Shaw J. G. 1990; The VPg of tobacco etch virus RNA is the 49-kDa proteinase or the N- terminal 24-kDa part of the proteinase. Virology 178:285–288
    [Google Scholar]
  24. Nicolas O., Laliberté J.-F. 1991; The use of PCR for the cloning of large cDNA fragments of turnip mosaic potyvirus. Journal of Virological Methods 32:57–66
    [Google Scholar]
  25. Nicolas O., Laliberté J.-F. 1992; The complete nucleotide sequence of turnip mosaic potyvirus RNA. Journal of General Virology 73:2785–2793
    [Google Scholar]
  26. Osman T. A. M., Hayes R. J., Buck K. W. 1992; Cooperative binding of the red clover necrotic mosaic virus movement protein to single-stranded nucleic acids. Journal of General Virology 73:223–227
    [Google Scholar]
  27. Osman T. A. M., Thömmes P., Buck K. W. 1993; Localization of a single-stranded RNA-binding domain in the movement protein of red clover necrotic mosaic dianthovirus. Journal of General Virology 74:2453–2457
    [Google Scholar]
  28. Poisson F., Roingeard P., Baillou A., Dubois F., Bonelli F., Calogero R. A., Goudeau A. 1993; Characterization of RNA- binding domains of hepatitis delta antigen. Journal of General Virology 74:2473–2477
    [Google Scholar]
  29. Riechmann J. L., Laín S., García J. A. 1992; Highlights and prospects of potyvirus molecular biology. Journal of General Virology 73:1–16
    [Google Scholar]
  30. Rodriguez-Cerezo E., Shaw J. G. 1991; Two newly-detected non-structural viral proteins in potyvirus infected cells. Virology 185:572–579
    [Google Scholar]
  31. Schoumacher F., Erny C., Berna A., Godefroy-Colburn T., Stussi-Garaud C. 1992; Nucleic acid-binding properties of the alfalfa mosaic virus movement protein produced in yeast. Virology 188:896–899
    [Google Scholar]
  32. Shukla D. D., Ward C. W. 1989; Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group. Advances in Virus Research 36:273–314
    [Google Scholar]
  33. Stern D. B., Gruissem W. 1987; Control of plastid gene expression: 3ʹ inverted repeats act as mRNA processing and stabilizing elements, but do not terminate transcription. Cell 51:1145–1157
    [Google Scholar]
  34. Studier F. W., Moffatt B. A. 1986; Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. Journal of Molecular Biology 189:113–130
    [Google Scholar]
  35. Thornbury D. W., Hellmann G. M., Rhoads R. E., Pirone T. P. 1985; Purification and characterization of potyvirus helper component. Virology 144:260–267
    [Google Scholar]
  36. Verchot J., Koonin E. V., Carrington J. C. 1991; The 35-kDa protein from the N-terminus of the potyviral polyprotein functions as a third virus-encoded proteinase. Virology 185:527–535
    [Google Scholar]
  37. Weeks K. M., Ampe C., Schultz S. C., Steitz T. A., Crothers D. M. 1990; Fragments of the HIV-1 tat protein specifically bind tar RNA. Science 249:1281–1285
    [Google Scholar]
  38. Yang J., Hunt A. G. 1992; Purification and characterization of a 70-kDa polyadenylate-binding protein from pea (Pisum sativum). Plant Physiology 98:1115–1120
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-75-10-2567
Loading
/content/journal/jgv/10.1099/0022-1317-75-10-2567
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error