1887

Abstract

An experimental scheme was developed for direct sequence analysis of Puumala virus-containing specimens from wild rodents (). Total RNA isolated from rodent lung tissues was reverse-transcribed in the presence of a universal 11 nucleotide primer complementary to all three viral RNA segments followed by amplification in a PCR with gene-specific primers. A full-length PCR product of approximately 1800 bp from the S segment encoding the viral nucleo-protein and a product of approximately 900 bp from the M segment (encoding the C-terminal two-thirds of the G2 protein and including the 3′ non-coding region) of Puumala virus (from trapped in Udmurtia) were prepared and sequenced. No pronounced differences to Vero cell-grown viruses were seen. The Udmurtia/894Cg/91 strain was more closely related to the Bashkiria/CG 18–20/84 strain than to the Finnish prototype strain of Puumala virus, Sotkamo/V-2969/81. Thus there is a correlation with the geographic origin of the three strains. The results indicate the occurrence of genetic drift and different selection pressures leading to (i) clustering of mutations, (ii) a lower frequency of nucleotide substitutions in the coding than in the 3′ noncoding regions and (iii) a higher frequency of amino acid substitutions in G2 than in the N protein.

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1994-02-01
2024-05-02
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References

  1. Antic D., Yong Kang C., Spik K., Schmaljohn C., Vapalahti O., Vaheri A. 1992; Comparison of the deduced gene products of the L, M and S genome segments of hantaviruses. Virus Research 24:35–46
    [Google Scholar]
  2. Becker Y., Darai G.editor 1992 Diagnosis of Human Viruses bv Polymerase Chain Reaction Technology Frontiers of Virology 1 Berlin-Heidelberg: Springer-Verlag;
    [Google Scholar]
  3. Brummer-Korvenkontio M., Vaheri A., Hovi T., Von Bonsdorff C. -H., Vuorimies J., Manni T., Penttinen K., Oker-Blom N., Lahdevirta J. 1980; Nephropathia epidemica: detection of antigen in bank voles and serological diagnosis of human infection. Journal of Infectious Diseases 141:131–134
    [Google Scholar]
  4. Brummer-Korvenkontio M., Henttonen H., Vaheri A. 1982; Hemorrhagic fever with renal syndrome in Finland: ecology and virology of nephropathia epidemica. Scandinavian Journal of Infectious Diseases Supplement 38:88–91
    [Google Scholar]
  5. Chumakov M. P., Gavrilovskay I. N., Boiko V. A., Zakharova M. A., Myasnikov YU. A., Bashkirev T. A., Apekina N. S., Safiullin R. S., Potapov V. S. 1981; Detection of hemorrhagic fever with renal syndrome (HFRS) virus in the lungs of bank voles Clethrionomys glareolus and redbacked voles Clethrionomys rutilis trapped in HFRS foci in the European part of U.S.S.R., and serodiagnosis of this infection in man. Archives of Virology 69:295–300
    [Google Scholar]
  6. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626
    [Google Scholar]
  7. Elliott R. M., Schmaljohn C. S., Collett M. S. 1991; Bunyaviridae genome structure and gene expression. Current Topics in Microbiology and Immunology 169:91–141
    [Google Scholar]
  8. Giebel L. B., Stohwasser R., Zöller L., Bautz E. K. F., Darai G. 1989; Determination of the coding capacity of the M segment of nephropatia epidemica virus strain Hällnäs B1 by molecular cloning and nucleotide sequence analysis. Virology 172:498–505
    [Google Scholar]
  9. Hedman K., Vaheri A., Brummer-Korvenkontio M. 1991; Rapid diagnosis of hantavirus disease with an IgG-avidity assay. Lancet 338:1353–1356
    [Google Scholar]
  10. Lee H. W., Lee P. W., Baek L. J., Chu Y. K. 1990; Geographical distribution of hemorrhagic fever with renal syndrome and hantaviruses. Archives of Virology Supplement 1:5–18
    [Google Scholar]
  11. Maddison W. P., Maddison D. R. 1992; MacClade: analysis of phylogeny and character evolution. Version 3.0. Sunderland, Massachusetts: Sinauer Associates;
    [Google Scholar]
  12. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  13. Stohwasser R., Giebel L. B., Raab K., Bautz E. K. F., Darai G. 1992; Polymerase chain reaction for detection of hantaviruses. In Diagnosis of Human Viruses by Polymerase Chain Reaction Technology.Frontiers of Virology 1 pp. 372–388 Becker Y., Darai G. Edited by Berlin-Heidelberg: Springer-Verlag;
    [Google Scholar]
  14. Tkatchenko E. A., Bashkirtsev V. N., Van Der Groen G., Dzagurova T. K., Ivanov A. P., Ryltseva E. V. 1984; Isolation in Vero E6 cells of hantavirus from Clethrionomys glareolus captured in Bashkiria area of the USSR. Annales de la Societé Beige de Medecine Tropicale 65:121–135
    [Google Scholar]
  15. Vapalahti O., Kallio-Kokko H., Salonen E. -M., Brummer-Korvenkontio M., Vaheri A. 1992; Cloning and sequencing of Puumala virus Sotkamo strain S and M RNA segments: evidence for strain variation in hantaviruses and expression of the nucleocapsid protein. Journal of General Virology 73:829–838
    [Google Scholar]
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