1887

Abstract

Purified virions of lettuce infectious yellows virus (LIYV), a tentative member of the closterovirus group, contained two RNAs of approximately 8500 and 7300 nucleotides (RNAs 1 and 2 respectively) and a single coat protein species with of approximately 28000. LIYV-infected plants contained multiple dsRNAs. The two largest were the correct size for the replicative forms of LIYV virion RNAs 1 and 2. To assess the relationships between LIYV RNAs 1 and 2, cDNAs corresponding to the virion RNAs were cloned. Northern blot hybridization analysis showed no detectable sequence homology between these RNAs. A partial amino acid sequence obtained from purified LIYV coat protein was found to align in the most upstream of four complete open reading frames (ORFs) identified in a LIYV RNA 2 cDNA clone. The identity of this ORF was confirmed as the LIYV coat protein gene by immunological analysis of the gene product expressed and in . Computer analysis of the LIYV coat protein amino acid sequence indicated that it belongs to a large family of proteins forming filamentous capsids of RNA plant viruses. The LIYV coat protein appears to be most closely related to the coat proteins of two clostero-viruses, beet yellows virus and citrus tristeza virus.

The nucleotide sequence reported in this paper has been submitted to the GenBank database and assigned the accession number U05242.

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1994-07-01
2024-05-21
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References

  1. Agranovsky A. A., Boyko V. P., Karasev A. V., Lunina N. A., Koonin E. V., Dolja V. V. 1991a; Nucleotide sequence of the 3′-terminal half of beet yellows closterovirus RNA genome: unique arrangement of eight virus genes. Journal of General Virology 72:15–23
    [Google Scholar]
  2. Agranovsky A. A., Boyko V. P., Karasev A. V., Koonin E. V., Dolja V. V. 1991b; Putative 65 kDa protein of beet yellows closterovirus is a homologue of HSP70 heat shock proteins. Journal of Molecular Biology 217:603–610
    [Google Scholar]
  3. Amasino R. M. 1986; Acceleration of nucleic acid hybridization rate by polyethylene glycol. Analytical Biochemistry 152:304–307
    [Google Scholar]
  4. Baltimore D. 1966; Purification and properties of poliovirus doublestranded ribonucleic acid. Journal of Molecular Biology 18:421–428
    [Google Scholar]
  5. Bar-Joseph M., Murant A. F. 1982; Closterovirus group. CMI/AAB Descriptions of Plant Viruses260
    [Google Scholar]
  6. Boyko V. P., Karasev A. V., Agranovsky A. A., Koonin E. V., Dolja V. V. 1992; Coat protein gene duplication in a filamentous RNA virus of plants. Proceedings of the National Academy of Sciences, U.S.A 89:9156–9160
    [Google Scholar]
  7. Burnett W. N. 1981; ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated Protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  8. Coffin R. S., Coutts R. H. A. 1993; The closteroviruses, capilloviruses and other similar viruses: a short review. Journal of General Virology 74:1475–1483
    [Google Scholar]
  9. Dawson W. O. 1983; Tobacco mosaic virus protein synthesis is correlated with double-stranded RNA synthesis and not singlestranded RNA synthesis. Virology 125:314–323
    [Google Scholar]
  10. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  11. Dolja V. V., Carrington J. C. 1992; Evolution of positive-strand RNA viruses. Seminars in Virology 3:315–326
    [Google Scholar]
  12. Dolja V. V., Boyko V. P., Agranovsky A. A., Koonin E. V. 1991; Phylogeny of capsid proteins of rod-shaped and filamentous RNA plant viruses: two families with distinct patterns of sequence and probably structure conservation. Virology 184:79–86
    [Google Scholar]
  13. Dougherty W. G., Hebert E. 1980; Translation of potyvirus RNA in a rabbit reticulocyte lysate: cell-free translation strategy and a genetic map of the potyviral genome. Virology 104:183–194
    [Google Scholar]
  14. Duffus J. E., Mayhew D. E., Flock R. A. 1982; Lettuce infectious yellows-a new whitefly-transmitted virus of the desert southwest. (Abstract). Phytopathology 72:963
    [Google Scholar]
  15. Duffus J. E., Larsen R. C., Liu H. Y. 1986; Lettuce infectious yellows virus-a new type of whitefly-transmitted virus. Phytopathology 76:97–100
    [Google Scholar]
  16. Felsenstein J. 1989; PHYLIP -phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  17. Feng D. F., Johnson M. S., Doolittle R. F. 1985; Aligning amino acid sequence: comparison of commonly used methods. Journal of Molecular Evolution 21:112–125
    [Google Scholar]
  18. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991; Classification and nomenclature of viruses. Fifth report of the International Committee on Taxonomy of Viruses. Archives of Virology Supplementum 2:
    [Google Scholar]
  19. German S., Candresse T., Lanneau M., Huet J. C., Pernollet J. C., Dunez J. 1990; Nucleotide sequence and genomic organization of apple chlorotic leaf spot closterovirus. Virology 179:104–112
    [Google Scholar]
  20. Gumpf D., Bar-Joseph M., Dodds J. A. 1981; Purification of citrus tristeza virus on sucrose-Cs2SO4 cushion gradients and estimation of its RNA size. Phytopathology 71:878
    [Google Scholar]
  21. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  22. Huiet L., Tsai J. H., Falk B. W. 1992; Complete sequence of maize stripe virus RNA4 and mapping of its subgenomic RNAs. Journal of General Virology 73:1603–1607 corrigendum 3049
    [Google Scholar]
  23. Kashiwazaki S., Hayano Y., Minobe Y., Omura T., Hibino H., Tsuchizaki T. 1989; Nucleotide sequence of the capsid protein gene of barley yellow mosaic virus. Journal of General Virology 70:3015–3023
    [Google Scholar]
  24. Kashiwazaki S., Minobe Y., Hibino H. 1991; Nucleotide sequence of barley yellow mosaic virus RNA 2. Journal of General Virology 72:995–999
    [Google Scholar]
  25. Klaassen V. A., Falk B. W. 1989; Characterization of a California isolate of sorghum yellow banding virus. Phytopathology 79:646–650
    [Google Scholar]
  26. Klaassen V. A., Boeshore M. L., Falk B. W. 1992; Molecular characterization of lettuce infectious yellows virus. (Abstract). Phytopathology 82:1111
    [Google Scholar]
  27. Lai M. M. C. 1992; RNA recombination in animal and plant viruses. Microbiological Reviews 56:61–79
    [Google Scholar]
  28. Lockhart B. E. L., Autrey L. J. C., Comstock J. C. 1992; Partial purification and serology of sugarcane mild mosaic virus, a mealybug-transmitted closterolike virus. Phytopathology 82:691–695
    [Google Scholar]
  29. Mcmaster G. K., Carmichael G. C. 1977; Analysis of single-and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U.S.A 74:4835–4838
    [Google Scholar]
  30. Morris T. J., Dodds J. A. 1979; Isolation and analysis of doublestranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858
    [Google Scholar]
  31. Ochi M., Kashiwazaki S., Hiratsuka K., Namba S., Tsuchizaki T. 1992; Nucleotide sequence of the 3′-terminal region of potato virus T RNA. Annals of the Phytopathological Society of Japan 58:416–425
    [Google Scholar]
  32. Pappu H. R., Karasev A. V., Anderson E. J., Pappu S. S., Lee R. F., Dolja V. V., Koonin E. V., Gumpf D. J., Niblett C. L. 1993; Citrus tristeza virus potentially encodes HSP70 and HSP90-like proteins. (Abstract). American Society for Virology 12th Annual Meeting A43:
    [Google Scholar]
  33. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  34. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  35. Sekiya M. E., Lawrence S. D., Mccaffery M., Cline K. 1991; Molecular cloning and nucleotide sequencing of the coat protein gene of citrus tristeza virus. Journal of General Virology 72:1013–1020
    [Google Scholar]
  36. Valverde R. A., Nameth S. T., Jordon R. L. 1990; Analysis of double-stranded RNA for plant virus diagnosis. Plant Disease 74:255–258
    [Google Scholar]
  37. Yoshikawa N., Sasaki E., Kato M., Takahashi T. 1992; The nucleotide sequence of apple stem grooving capillovirus genome. Virology 191:98–105
    [Google Scholar]
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