1887

Abstract

Enhancin, an infectivity-enhancing protein from granulosis virus (TnGV) was tested for its ability to increase multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV) infection in the larvae of four lepidopteran insects. Enhancin increased the mortality of AcMNPV infection in all the four insect species tested. Peritrophic membrane (PM) assays showed altered protein profiles in PMs treated with enhancin in all the four species. This supports the hypothesis that enhancin affects virus infection by altering the structural integrity of the PMs. The binding of enhancin to the midgut brush border membranes (BBMs) was determined and specific binding sites were found on the BBM of No specific binding sites were found on the BBMs of or Therefore, specific binding of enhancin to the midgut cell membrane may not be necessary for the enhancement of baculovirus infection in insects.

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1994-08-01
2024-04-30
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References

  1. Bell R. A., Owens C. D., Shapiro M., Tardif J. R. 1981; Development of mass-rearing technology. In The Gypsy Moth: Research Toward Integrated Pest Management pp. 599–633 Doane C. C., McManus M. L. Edited by Washington, D.C: U.S. Department of Agriculture;
    [Google Scholar]
  2. Blum H., Beier H., Gross H. J. 1987; Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8:93–99
    [Google Scholar]
  3. Corsaro B. G., Gijzen M. R., Wang P., Granados R. R. 1993; Baculovirus enhancing proteins as determinants of viral pathogenesis. In Parasites and Pathogens of Insects 2 pp. 127–145 Beckage N., Thompson S. N., Federici B. A. Edited by New York: Academic Press;
    [Google Scholar]
  4. Derksen A. C. D., Granados R. R. 1988; Alteration of a lepidopteranperitrophic membrane by baculoviruses and enhancement of viral infection. Virology 167:242–250
    [Google Scholar]
  5. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991; Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology Supplementum 2 Wien: Springer-Verlag;
    [Google Scholar]
  6. Gallo L. G., Corsaro B. G., Hughes P. R., Granados R. R. 1991; In vivo enhancement of baculovirus infections by the viral enhancing factor of a granulosis virus of the cabbage looper, Trichoplusia ni (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology 58:203–210
    [Google Scholar]
  7. Goto C. 1990; Enhancement of a nuclear polyhedrosis virus (NPV) infection by a granulosis virus (GV) isolated from the spotted cutworm, Xestia c-nigrum L. (Lepidoptera: Noctuidae). Applied Entomology and Zoology 25:135–137
    [Google Scholar]
  8. Granados R. R., Corsaro B. G. 1990; Baculovirus enhancing proteins and their implication for insect control. In Proceedings and Abstracts of the Fifth International Colloquium, on Invertebrate Pathology and Microbial Control pp. 174–178
    [Google Scholar]
  9. Hara S., Tanada Y., Omi E. M. 1976; Isolation and characterization of a synergistic enzyme from the capsule of a granulosis virus of the armyworm. Pseudaletia unipuncta. Journal of Invertebrate Pathology 21:115–124
    [Google Scholar]
  10. Hashimoto Y., Corsaro B. G., Granados R. R. 1991; Location and nucleotide sequence of the gene encoding the viral enhancing factor of the Trichoplusia ni granulosis virus. Journal of General Virology 72:2645–2651
    [Google Scholar]
  11. Hukuhara T., Tamura K., Zhu Y., Abe H., Tanada Y. 1987; Synergistic factor shows specificity in enhancing nuclear polyhedrosis virus infections. Applied Entomology and Zoology 22:235–236
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  13. Quisenberry S. S., Whitford F. 1988; Evaluation of bermuda-grass resistance to fall armyworm (Lepidoptera: Noctuidae): influence of host strain and dietary conditioning. Journal of Economic Entomology 81:1463–1468
    [Google Scholar]
  14. Ryerse J. S., Purcell J. P., Sammons R. D., Lavrik P. B. 1992; Peritrophic membrane structure and formation in the larva of a moth, Heliothis. Tissue and Cell 24:751–771
    [Google Scholar]
  15. Santos C. D., Ferreira C., Terra W. R. 1983; Consumption of food and spatial organization of digestion of the cassava hornworm, Erinnyis ello. Journal of Insect Physiology 29:707–714
    [Google Scholar]
  16. Tanada Y. 1959; Synergism between viruses of the armyworm, Pseudaletia unipuncta (Haworth) (Lepidoptera, Noctuidae). Journal of Invertebrate Pathology 1:215–231
    [Google Scholar]
  17. Tanada Y. 1985; A synposis of studies on the synergistic property of an insect baculovirus: a tribute to Edward A. Steinhaus. Journal of Invertebrate Pathology 45:125–138
    [Google Scholar]
  18. Tanada Y., Himeno M., Omi E. M. 1973; Isolation of a factor, from the capsule of a granulosis virus, synergistic for a nuclear-polyhedrosis virus of the armyworm. Journal of Invertebrate Pathology 21:31–40
    [Google Scholar]
  19. Tanada Y., Hess R. T., Omi E. M. 1975; Invasion of nuclear polyhedrosis virus in midgut of the armyworm. Pseudaletia unipuncta, and the enhancement of a synergistic enzyme. Journal of Invertebrate Pathology 26:99–104
    [Google Scholar]
  20. Tanada Y., Inoue H., Hess R. T., Omi E. M. 1980; Site of action of synergistic factor of a granulosis virus of the armyworm, Pseudaletia unipuncta. Journal of Invertebrate Pathology 34:249–255
    [Google Scholar]
  21. Tanada Y., Hess R. T., Omi E. M., Yamamoto T. 1983; Localization of a synergistic factor of a granulosis virus by its esterase activity in the larval midgut of the armyworm, Pseudaletia unipuncta. Microbios 37:87–93
    [Google Scholar]
  22. Uchima K., Harvey J. P., Omi E. M., Tanada Y. 1988; Binding sites on the midgut cell membrane for the synergistic factor of a granulosis virus of the armyworm (Pseudaletia unipuncta). Insect Biochemistry 18:645–650
    [Google Scholar]
  23. Vail P. V., Vail S. S. 1987; Comparative replication of Autographa californica nuclear polyhedrosis virus in tissues of Heliothis spp. (Lepidoptera: Noctuidae). Annals of the Entomological Society of America 80:734–738
    [Google Scholar]
  24. Wolfersberger M., Luethy P., Maurer A., Parenti P., Sacchi F. V., Giordana B., Hanozet G. M. 1987; Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the cabbage butterfly (Pieris brassicae). Comparative Biochemistry and Physiology 86A:301–308
    [Google Scholar]
  25. Wood H. A. 1980; Autographa californica nuclear polyhedrosis virus-induced proteins in tissue culture. Virology 102:21–27
    [Google Scholar]
  26. Yunovitz H., Sneh B., Yawetz A. 1987; The pH characterizing the cytolytic activity of Bacillus thuringiensis δ-endotoxin in the larval epithelium of Spodoptera littoralis. Journal of Invertebrate Pathology 50:320–321
    [Google Scholar]
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