f Characterization of Mapuera virus: structure, proteins and nucleotide sequence of the gene encoding the nucleocapsid protein
- Authors: G. W. Henderson, C. Laird, E. Dermott, B. K. Rima*
*Author for correspondence. Fax +44 1232 236505. e-mail B.Rima@qub.ac.uk.
- First Published Online: 01 October 1995, Journal of General Virology 76: 2509-2518, doi: 10.1099/0022-1317-76-10-2509
- Subject: Animal
- Issue Published:
The molecular biology of Mapuera virus was studied at both the protein and nucleic acid levels. Seven virus-encoded proteins were detected in infected Vero cells. The sizes and characteristics of each of the proteins determined from various radiolabelling experiments allowed preliminary identification of the proteins as the large (L; 190 kDa), haemagglutinin neuraminidase (HN; 74 kDa), nucleocapsid (N; 66 kDa), fusion (F0; 63 kDa), phosphoprotein (P; 49 kDa), matrix (M; 43 kDa) and non-structural (V; 35 kDa) proteins. Western blot analysis showed that the HN, N and P proteins were major antigens recognized in the mouse. A cDNA library of total virus-infected cellular mRNA was created and screening of the library resulted in the detection of cDNA sequences representing the N mRNA transcript of Mapuera virus. The N mRNA sequence determined from the clones was 1731 nt in length and contained an ORF that encoded 537 amino acids, the complete 3′ untranslated region and part of the 5′ non-coding region. The calculated M r of the N protein was 59 kDa, which is close to the 66 kDa protein observed by SDS-PAGE.
Author for correspondence. Fax +44 1232 236505. e-mail B.Rima@qub.ac.uk.
© Society for General Microbiology 1995 | Published by the Microbiology Society
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