f Comparison of the genomes of the wild-type French viscerotropic strain of yellow fever virus with its vaccine derivative French neurotropic vaccine
- Authors: Eryu Wang, Kate D. Ryman, Alan D. Jennings*, David J. Wood, Frank Taffs, Philip D. Minor, Peter G. Sanders, Alan D. T. Barrett‡
*Author for correspondence. Fax +1 409 747 2415. e-mail email@example.com
- First Published Online: 01 November 1995, Journal of General Virology 76: 2749-2755, doi: 10.1099/0022-1317-76-11-2749
- Subject: Animal
- Issue Published:
The French neurotropic vaccine, or FNV, was used extensively in Africa to control yellow fever (YF). Although efficacious, the vaccine caused an unacceptable rate of post-vaccinal complications in children and was subsequently replaced by the 17D vaccine. Here we report that the genomes of the wild-type YF virus French viscerotropic virus and its attenuated vaccine derivative, FNV virus from the Institut Pasteur, Paris, (FNV-IP) differ by 77 nucleotides encoding 35 amino acid substitutions. Comparison of FNV-IP and three other isolates of FNV with other YF vaccine strains (17D-204 and 17DD derived from wild-type strain Asibi) revealed that during the two attenuation processes two common nucleotide changes arose that encode two amino acid substitutions: one is in the membrane protein at amino acid 35 (M-35), the other in nonstructural (NS) protein 4B at NS4B-95. These common substitutions may be important in the process of attenuation of viscerotropic disease for humans and monkeys, and/or may be involved in loss of mosquito competence of the vaccine viruses.
Author for correspondence. Fax +1 409 747 2415. e-mail firstname.lastname@example.org
Present address: European Collection of Animal Cell Cultures, Centre for Applied Microbiology and Research, Salisbury, Wiltshire SP4 0JG, UK.
© Society for General Microbiology 1995 | Published by the Microbiology Society
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