1887

Abstract

The Epstein—Barr virus (EBV) BZLF1 gene product, Zta is able to trigger the viral lytic cycle in latently infected B lymphocytes. Investigations into regulation of the promoter, Zp for the BZLF1 gene in B cells have identified 12--tetradecanoylphorbol 13-acetate, anti-immunoglobulin and Zta responsive elements as well as a negative regulatory element within Zp. EBV infects and replicates within squamous epithelial cells and there is evidence to indicate that EBV gene expression is linked to the differentiation status of epithelial cells. We have investigated regulation of Zp in undifferentiated and differentiated cells of the human squamous epithelial cell line SCC12F. Zp was found to be active in SCC12F cells and activity was increased approximately 7-fold upon induction of epithelial terminal differentiation. Sequences responsive to epithelial cell differentiation were contained within the region of Zp from -86 to +12 bp, a region previously shown to contain an AP-1-like binding site, designated the ZII domain. In addition, enhancing sequences were detected in the region -221 to -86 bp. These studies will lead to a greater understanding of differentiation-linked EBV gene expression in human squamous epithelial cells.

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1995-04-01
2024-05-02
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References

  1. Chevallier-Greco A., Manet E., Chavrier P., Mosnier C., Daillie J., Sergeant A. 1986; Both Epstein Barr virus (EBV)-encoded trans-acting factors, EB1 and EB2, are required to activate transcription from an EBV early promoter. EMBO Journal 5:3243–3249
    [Google Scholar]
  2. Countryman J., Miller G. 1985; Activation of expression of latent Epstein-Barr herpesvirus after gene transfer with a small cloned subfragment of heterogeneous viral DNA. Proceedings of the National Academy of Sciences, USA 82:4085–4089
    [Google Scholar]
  3. Crawford D. H., Ando I. 1986; EB virus induction is associated with B cell maturation. Immunology 59:405–409
    [Google Scholar]
  4. Dover R., Watt F. M. 1987; Measurement of the rate of epidermal terminal differentiation: expression of involucrin by S-phase keratinocytes in culture and in psoriatic plaques. Journal of Investigative Dermatology 89:349–352
    [Google Scholar]
  5. Flemington E., Speck S. H. 1990a; Identification of phorbol ester response elements in the promoter of Epstein-Barr virus putative lytic switch gene BZLF1. Journal of Virology 64:1217–1226
    [Google Scholar]
  6. Flemington E., Speck S. H. 1990b; Autoregulation of Epstein-Barr virus putative lytic switch gene BZLF1. Journal of Virology 64:1227–1232
    [Google Scholar]
  7. Folkman J., Moscona A. 1978; Role of cell shape in growth control. Nature 273:345–349
    [Google Scholar]
  8. Golden H. D., Chang R. S., Prescott W., Simpson E., Cooper T. Y. 1973; Leukocyte-transforming agent: prolonged excretion by patients with mononucleosis and excretion by normal individuals. Journal of Infectious Diseases 127:471–473
    [Google Scholar]
  9. Gorman C. M., Moffat L. F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  10. Green H. 1977; Terminal differentiation of cultured human epidermal cells. Cell 11:405–416
    [Google Scholar]
  11. Henle G., Henle W., Diehl V. 1968; Relation of Burkitt’s tumour-associated herpes-type virus to infectious mononucleosis. Proceedings of the National Academy of Sciences, USA 59:94–101
    [Google Scholar]
  12. Israele V., Shirley P., Sixbey J. W. 1991; Excretion of the Epstein-Barr virus from the genital tract of men. Journal of Infectious Diseases 163:1341–1343
    [Google Scholar]
  13. Kieff E., Lebowitz D. 1991; Epstein-Barr virus and its replication. In Fundamental Virology 2nd edn pp 897–928 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  14. Li Q. X., Young L. S., Nedobitek G., Dawson C. W., Birken-bach M., Wang F., Rickinson A. B. 1992; Epstein-Barr virus infection and replication in a human epithelial cell system. Nature 356:347–350
    [Google Scholar]
  15. Luka J., Kallin B., Klein G. 1979; Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate. Virology 94:228–231
    [Google Scholar]
  16. Miller G. 1990; Epstein-Barr virus. Biology, pathogenesis and medical aspects. In Virology 2nd edn, pp 1921–1958 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  17. Montalvo E. A., Shi Y., Shenk T. E., Levine A. J. 1991; Negative regulation of the BZLF1 promoter of Epstein-Barr virus. Journal of Virology 65:3647–3655
    [Google Scholar]
  18. Nederman J. C., Evans A. S., Subrahmanyan L., McCollum R. W. 1970; Prevalence, incidence and persistence of EB virus antibody in young adults. New England Journal of Medicine 282:361–365
    [Google Scholar]
  19. Nedobitek G., Young L. S. 1994; Epstein-Barr virus persistence and virus-associated tumours. Lancet 343:333–335
    [Google Scholar]
  20. Pattengale P. K., Smith R. W., Gerber P. 1973; Selective transformation of B lymphocytes by EB virus. Lancet ii:93–94
    [Google Scholar]
  21. Pope J. H., Horne M. K., Scott W. 1968; Transformation of foetal human leucocytes in vitro by filtrates of a human leukaemia cell line containing herpes-like virus. International Journal of Cancer 3:857–866
    [Google Scholar]
  22. Rheinwald J. G. 1980; Serial cultivation of normal human epidermal keratinocytes. Methods in Cell Biology 21A:229–254
    [Google Scholar]
  23. Rheinwald J. G., Beckett M. A. 1980; Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22:629–632
    [Google Scholar]
  24. Rheinwald J. G., Green H. 1975; Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6:331–334
    [Google Scholar]
  25. Rice R. H., Green H. 1979; Presence in human epidermal cells of a soluble protein precursor of the cross-linked envelope: activation of the cross-linking by calcium ions. Cell 18:681–694
    [Google Scholar]
  26. Schwarz E., Freese U. K., Gissmann L., Mayer W., Roggenbuck B., Stremlau A., zur Hausen H. 1985; Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature 314:111–114
    [Google Scholar]
  27. Shelbourn S. L., Sissons J. G. P., Sinclair J. H. 1989; Expression of oncogenic ras in human teratocarcinoma cells induces partial differentiation and permissiveness for human cytomegalovirus infection. Journal of General Virology 70:367–374
    [Google Scholar]
  28. Shimizu N., Takada K. 1993; Analysis of the BZLF1 promoter of Epstein-Barr virus: identification of an anti-immunoglobulin response sequence. Journal of Virology 67:3240–3245
    [Google Scholar]
  29. Sixbey J. W. 1989; Epstein-Barr virus and epithelial cells. In Advances in Viral Oncology pp 187–202 Edited by Klein G. New York: Raven Press;
    [Google Scholar]
  30. Sixbey J. W., Vesterinen E. H., Nedrud J. G., Raab-Traub N., Walton L. A., Pagano J. S. 1983; Replication of Epstein-Barr virus in human epithelial cells infected in vitro . Nature 306:480–483
    [Google Scholar]
  31. Sixbey J. W., Nedrud J. G., Raab-Traub N., Hanes R. A., Pagano J. S. 1984; Epstein-Barr virus replication in oropharyngeal epithelial cells. New England Journal of Medicine 310:1225–1230
    [Google Scholar]
  32. Sixbey J. W., Lemon S. M., Pagano J. S. 1986; A second site for Epstein-Barr virus shedding: the uterine cervix. Lancet ii:1122–1124
    [Google Scholar]
  33. Takada K., Shimizu N., Sakuma S., Ono Y. 1986; trans Activation of the latent Epstein-Barr virus (EBV) genome after transfection of the EBV DNA fragment. Journal of Virology 57:1016–1022
    [Google Scholar]
  34. Tovey M. G., Lenoir G., Begon-Lours J. 1978; Activation of latent Epstein-Barr virus by antibody to human IgM. Nature 276:270–272
    [Google Scholar]
  35. Urier G., Buisson M., Chambard P., Sergeant A. 1989; The Epstein-Barr virus early protein EB1 activates transcription from different responsive elements including AP-1 binding sites. EBMO Journal 8:1447–1453
    [Google Scholar]
  36. Zur Hausen H., O’Neill F. J., Freese U. K. 1978; Persisting oncogenic herpesvirus induced by the tumour promoter TPA. Nature 272:373–375
    [Google Scholar]
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