In vitro cleavage of hepatitis C virus polyprotein substrates by purified recombinant NS3 protease

References

1. Bartenschlager R., Ahlborn-Laake L., Mous J., Jacobsen H. 1993; Nonstructural protein 3 of the hepatitis C virus encodes a serine-type proteinase required for cleavage at the NS3/4 and NS4/5 junctions. Journal of Virology 67:3835–3844
   [Google Scholar]
2. Bartenschlager R., Ahlborn-Laake L., Mous J., Jacobsen H. 1993; Kinetic and structural analyses of hepatitis C virus polyprotein processing. Journal of Virology 68:5045–5055
   [Google Scholar]
3. Choo Q.-L., Richman K. H., Han J. H., Berger K., Lee C., Dong C., Gallegos C., Coit D., Medina-Selby A., Barr P. J., Weiner A. J., Bradley D. W., Kuo G., Houghton M. 1991; Genetic organization and diversity of the hepatitis C virus. Proceedings of the National Academy of Sciences, USA 88:2451–2455
   [Google Scholar]
4. Choo Q. L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. 1989; Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359–362
   [Google Scholar]
5. D’Souza E. D. A., O’Sullivan E., Amphlett E. M., Rowlands D. J., Sangar D. V., Clarke B. E. 1994; Analysis of NS3-mediated processing of the hepatitis C virus non-structural region in vitro . Journal of General Virology 75:3469–3476
   [Google Scholar]
6. Eckart M. R., Selby M., Masiarz F., Lee C., Berger K., Crawford K., Kuo G., Houghton M., Choo Q.-L. 1993; The hepatitis C virus encodes a serine protease involved in processing of the putative non-structural proteins from the viral polyprotein precursor. Biochemical and Biophysical Research Communications 192:399–406
   [Google Scholar]
7. Failla C., Tomei L., de Francesco R. 1994; Both NS3 and NS4A are required for proteolytic processing of hepatitis C virus nonstructural proteins. Journal of Virology 68:3753–3760
   [Google Scholar]
8. Grakoui A., Wychowski C., Lin C., Feinstone S. M., Rice C. M. 1993a; Expression and identification of hepatitis C virus polyprotein cleavage products. Journal of Virology 67:1385–1395
   [Google Scholar]
9. Grakoui A., McCourt D. W., Wychowski C., Feinstone S. M., Rice C. M. 1993b; Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites. Journal of Virology 67:2832–2843
   [Google Scholar]
10. Hijikata M., Kato N., Ootsuyama Y., Nakagawa M., Shimotohno K. 1991; Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis. Proceedings of the National Academy of Sciences, USA 88:5547–5551
    [Google Scholar]
11. Hijikata M., Mizushima H., Akagi T., Mori S., Kakiuchi N., Kato N., Tanaka T., Kimura K., Shimotohmo K. 1993; Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus. Journal of Virology 67:4665–1675
    [Google Scholar]
12. Kato N., Hijikata M., Ootsuyama Y., Nakagawa M., Ohkoshi S., Sugimura T., Shimotohno K. 1990; Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proceedings of the National Academy of Sciences, USA 87:9524–9528
    [Google Scholar]
13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
14. Miller R. H., Purcell R. H. 1990; Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups. Proceedings of the National Academy of Sciences, USA 87:2057–2061
    [Google Scholar]
15. Suzich J. A., Tamura J. K., Palmer-Hill F., Warrener P., Grakoui A., Rice C. M., Feinstone S. M., Collett M. S. 1993; Hepatitis C virus NS3 protein polynucleotide-stimulated nucleoside triphosphatase and comparison with the related pestivirus and flavivirus enzymes. Journal of Virology 67:6152–6158
    [Google Scholar]
16. Takamizawa A., Mori C., Fuke I., Manabe S., Murakami S., Fujita J., Onishi E., Andoh T., Yoshida I., Okayama H. 1991; Structure and organisation of the hepatitis C virus genome isolated from human carriers. Journal of Virology 65:1105–1113
    [Google Scholar]
17. Tomei L., Failla C., Santolini E., de Francesco R., la Monica N. 1993; NS3 is a serine protease required for processing of hepatitis C virus polyprotein. Journal of Virology 67:4017–4026
    [Google Scholar]