f Isolation and characterization of Tula virus, a distinct serotype in the genus Hantavirus, family Bunyaviridae
- Authors: Olli Vapalahti, Åke Lundkvist, Sami K. J. Kukkonen, Ying Cheng, Mari Gilljam, Mari Kanerva, Tytti Manni, Milan Pejcoch, Jukka Niemimaa, Asko Kaikusalo, Heikki Henttonen, Antti Vaheri, Alexander Plyusnin
- J. Gen. Virol., December 1996 77: 3063-3067, doi: 10.1099/0022-1317-77-12-3063
- Subject: Animal - RNA viruses
- Published Online:
A Vero E6 cell culture isolate of Tula virus (TUL), a hantavirus first detected in European common voles (Microtus arvalis and M. rossiaemeridionalis) by RT-PCR was obtained after initial passaging of TUL-infected vole lung samples in laboratory-colonized M. arvalis. TUL was defined as a classical serotype by a cross-focus-reduction neutralization test (FRNT) and was also shown to be distinct from other hantaviruses by haemagglutination inhibition assay. The sequences of S, M and partial L genome segments of the isolate were determined: the S segment was 99·9% identical to the original rodent-derived sequence. Serological evidence for a previous TUL infection was obtained from the serum of a blood donor living near a TUL focus in Moravia, Czech Republic, showing at least a 16-fold higher FRNT titre to TUL as compared to Puumala or other hantaviruses.
© Society for General Microbiology Ltd 1996 | Published by the Microbiology Society
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