1887

Journal of General Virology header logo

Volume 78, Issue 7

Role of gamma delta TCR+ lymphocytes in the augmented resistance of trehalose 6,6'-dimycolate-treated mice to influenza virus infection Free

Abstract

Trehalose 6,6′-dimycolate (TDM), an immunomodu- lator, potentiates non-specific resistance in mice to influenza virus infection. When mice were injected intravenously with TDM, the striking proliferation of a minority of T-lymphocytes bearing gamma/delta T-cell receptors ( T-cells) that accumulated in granulomatous lungs was thought to be associated with the maintenance of acquired resistance to lethal influenza virus infection. To clarify the cellular basis of the defence against influenza virus, mice were depleted of T-cells, alpha/beta (ap) T-cells, or natural killer (NK) cells by administration of corresponding antibodies prior to influenza virus infection. The depletion of T-cells significantly abrogated the augmented resistance of TDM- treated mice to infection, as did depletion of either α T-cells or NK cells. To gain insight into the functional ability of T-cells, we evaluated the cytotoxic activity ofthisT-cell subset againsta panel of target cell lines that were stably transfected with the influenza virus haemagglutinin (HA) gene from A/PR/8/34(H1N1) and A/Aichi/2/68(H3N2) strains. The T-cells from TDM-treated mice showed profound cytotoxicity against the target cells expressing HA of either the H1 or H3 subtype, in a non-major histocompatibility complex-restricted manner. Taken together, these results indicate that T-cells play a non-specific role, in conjunction with α T-cells and NK cells, in protecting mice against influenza virus infection, and that the recognition and destruction of HA-expressing target cells by the activated T-cells is one of the steps involved in this anti-influenza virus immuno- surveillance.

  • Published Online:
© Society for General Microbiology 1997
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-7-1597
1997-07-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/7/9225035.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-7-1597&mimeType=html&fmt=ahah

References

  1. Allan W., Carding S. R., Eichelberger M., Doherty P. C. 1992; Analysing the distribution of cells expressing mRNA for T cell receptor γ and δ chains in a virus-induced inflammatory process. Cellular Immunology 143:55–65
     [Google Scholar]
  2. Andersson S., Davis D. L., Dahlbäck H., Jörnvall H., Russell D. W. 1989; Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme. Journal of Biological Chemistry 264:8222–8229
     [Google Scholar]
  3. Augustin A., Kubo R. T., Sim G. K. 1989; Resident pulmonary lymphocytes expressing the γ/δ T-cell receptor. Nature 340:239–241
     [Google Scholar]
  4. Azuma M., Suzutani T., Sazaki K., Yoshida I., Sakuma T., Yoshida T. 1987; Role of interferon in the augmented resistance of trehalose 6,6ʹ-dimycolate-treated mice to influenza virus infection. Journal of General Virology 68:835–843
     [Google Scholar]
  5. Azuma M., Sazaki K., Nishikawa Y., Takahashi T., Shimoda A., Suzutani T., Yoshida I., Sakuma T., Nakaya K. 1988; Correlation between augmented resistance to influenza virus infection and histological changes in lung of mice treated with trehalose 6,6ʹ-dimycolate. Journal of Biological Response Modifiers 7:473–482
     [Google Scholar]
  6. Ellens H., Bentz J., Mason D., Zhang F., White J. M. 1990; Fusion of influenza hemagglutinin-expressing fibroblasts with glycophorin- bearing liposomes: role of hemagglutinin surface density. Biochemistry 29:9697–9707
     [Google Scholar]
  7. Felgner P. L., Holm M. 1989; Cationic liposome-mediated transfection. Focus 11:21–25
     [Google Scholar]
  8. Goodwin C. J., Holt S. J., Downes S., Marshall N. J. 1995; Microculture tetrazolium assays : a comparison between two new tetrazolium salts, XTT and MTS. Journal of Immunological Methods 179:95–103
     [Google Scholar]
  9. Haas W., Pereira P., Tonegawa S. 1993; Gamma/delta cells. Annual Review of Immunology 11:637–685
     [Google Scholar]
  10. Hiromatsu K., Yoshikai Y., Matsuzaki G., Ohga S., Muramori K., Matsumoto K., Bluestone J. A., Nomoto K. 1992; A protective role of ;δ T cells in primary infection with Listeria monocytogenes in mice. Journal of Experimental Medicine 175:49–56
     [Google Scholar]
  11. Johansson B. E., Bucher D. J., Kilbourne E. D. 1989; Purified influenza virus hemagglutinin and neuraminidase are equivalent in stimulation of antibody response but induce contrasting types of immunity to infection. Journal of Virology 63:1239–1246
     [Google Scholar]
  12. Johnson R. M., Lancki D. W., Sperling A. I., Dick R. F., Spear P. G., Fitch F. W., Bluestone J. A. 1992; A murine CD4-, CD8- T cell receptor-;δ T lymphocyte clone specific for herpes simplex virus glycoprotein I. Journal of Immunology 148:983–988
     [Google Scholar]
  13. Justewicz D. M., Doherty P. C., Webster R. G. 1995; The B-cell response in lymphoid tissue of mice immunized with various antigenic forms of the influenza virus hemagglutinin. Journal of Virology 69:5414–5421
     [Google Scholar]
  14. Klein J. L., Fickenscher H., Holliday J. E., Biesinger B., Fleckenstein B. 1996; Herpesvirus saimiri immortalized γδ T cell line activated by IL-12. Journal of Immunology 156:2754–2760
     [Google Scholar]
  15. Ladel C. H., Hess J., Daugelat S., Mombaerts P., Tonegawa S., Kaufmann S. H. E. 1995; Contribution of α/β and γ/δ T lymphocytes to immunity against Mycobacterium bovis Bacillus Calmette-Guérin: studies with T-cell receptor-deficient mutant mice. European Journal of Immunology 25:838–846
     [Google Scholar]
  16. Ladel C. H., Blum C., Kaufmann S. H. E. 1996; Control of natural killer cell-mediated innate resistance against the intracellular pathogen Listeria monocytogenes by γδ T lymphocytes. Infection and Immunity 64:1744–1749
     [Google Scholar]
  17. Munk M. E., Gatrill A. J., Kaufmann S. H. E. 1990; Target cell lysis and IL-2 secretion by γ/δ T lymphocytes after activation with bacteria. Journal of Immunology 145:2434–2439
     [Google Scholar]
  18. Nilssen D. E., Müller F., Øktedalen O., Frøland S. S., Fausa O., Halstensen T. S., Brandtzaeg P. 1996; Intraepithelial γ/δ T cells in duodenal mucosa are related to the immune state and survival time in AIDS. Journal of Virology 70:3545–3550
     [Google Scholar]
  19. Ogasawara T., Emoto M., Kiyotani K., Shimokata K., Yoshida T., Nagai Y., Yoshikai Y. 1994; Sendai virus pneumonia: evidence for the early recruitment of γδ T cells during the disease course. Journal of Virology 68:4022–4027
     [Google Scholar]
  20. Perussia B., Chan S. H., D’Andrea A., Tsuji K., Santoli D., Pospisil M., Young D., Wolf S. F., Trinchieri G. 1992; Natural killer (NK) cell stimulatory factor or IL-12 has differential effects on the proliferation ofTCR-αβ +, TCR-γδ+ T lymphocytes, and NK cells. Journal of Immunology 149:3495–3502
     [Google Scholar]
  21. Ponniah S., Doherty P. C., Eichelberger M. 1996; Selective response of γδ T-cell hybridomas to orthomyxovirus-infected cells. Journal of Virology 70:17–22
     [Google Scholar]
  22. Rosat J. P., MacDonald H. R., Louis J. A. 1993; A role for γδ + T cells during experimental infection of mice with Leishmania major . Journal of Immunology 150:550–555
     [Google Scholar]
  23. Sazaki K., Yoshida I., Azuma M. 1992; Mechanisms of augmented resistance of cyclosporin A-treated mice to influenza virus infection by trehalose 6,6ʹ-dimycolate. Microbiology and Immunology 36:1061–1075
     [Google Scholar]
  24. Sciammas R., Johnson R. M., Sperling A. I., Brady W., Linsley P. S., Spear P. G., Fitch F. W., Bluestone J. A. 1994; Unique antigen recognition by a herpesvirus-specific TCR-yd cell. Journal of Immunology 152:5392–5397
     [Google Scholar]
  25. Simeckova-Rosenberg J., Yun Z., Wyde P. R., Atassi M. Z. 1995; Protection of mice against lethal viral infection by synthetic peptides corresponding to B- and T-cell recognition site of influenza A hemagglutinin. Vaccine 13:927–932
     [Google Scholar]
  26. Skoner D. P., Whiteside T. L., Wilson J. W., Doyle W. J., Herberman R. B., Fireman P. 1996; Effect of influenza A virus infection on natural and adaptive cellular immunity. Clinical Immunology and Immuno- pathology 79:294–302
     [Google Scholar]
  27. Stein-Streilein J., Guffee J., Fan W. 1988; Locally and systemically derived natural killer cells participate in defense against intranasally inoculated influenza virus. Regional Immunology 1:100–105
     [Google Scholar]
  28. Tsuji M., Mombaerts P., Lefrancois L., Nussenzweig R. S., Zavala F., Tonegawa S. 1994; γδ T cells contribute to immunity against the liver stages of malaria in αβ T-cell-deficient mice. Proceedings of the National Academy of Sciences USA: 91345–349
     [Google Scholar]
  29. Wallace M., Malkovsky M., Carding S. R. 1995; Gamma/delta T lymphocytes in viral infections. Journal of Leukocyte Biology 58:277–283
     [Google Scholar]
  30. Zhang M., Gately M. K., Wang E., Gong J., Wolf S. F., Lu S., Modlin R. L., Barnes P. F. 1994; Interleukin 12 at the site of disease in tuberculosis. Journal of Clinical Investigation 93:1733–1739
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-7-1597
Loading
Role of gamma delta TCR+ lymphocytes in the augmented resistance of trehalose 6,6'-dimycolate-treated mice to influenza virus infection
J. Gen. Virol. 78, 1597 (1997); https://doi.org/10.1099/0022-1317-78-7-1597
/content/journal/jgv/10.1099/0022-1317-78-7-1597
/content/journal/jgv/10.1099/0022-1317-78-7-1597
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error