1887

Abstract

Pseudotype formation is a powerful tool for analysing mechanisms of virus neutralization and entry, since it allows for analysis of glycoprotein properties without the necessity for preparing recombinant genomes. Using recombinant vaccinia viruses, we prepared pseudotypes of La Crosse virus with recombinant glycoproteins cloned from the monoclonal antibody (MAb)-resistant variant V31. The resulting pseudotypes became partially resistant to MAb 807–31. Furthermore, when the V31 glycoproteins were incorporated into a second MAb-resistant variant (V33), the pseudotyped virus became sensitive to neutralization by the MAb (807–33) originally used in its selection. These results suggest a simple technique for the incorporation of glycoprotein mutations into bunya-viruses, allowing analysis of mechanisms of neutralization and other virus entry functions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-4-667
1998-04-01
2024-05-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/4/9568959.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-4-667&mimeType=html&fmt=ahah

References

  1. Bupp K., Stillmock K., González-Scarano F. 1996; Analysis of the intracellular transport properties of recombinant La Crosse virus glycoproteins. Virology 220:485–490
    [Google Scholar]
  2. Bridgen A., Elliott R. M. 1996; Rescue of a segmented negative-strand RNA virus entirely from cloned complementary DNAs. Proceedings of the National Academy of Sciences, USA 93:15400–15404
    [Google Scholar]
  3. Chakrabarti S., Brechling K., Moss B. 1985; Vaccinia virus expression vector: coexpression of β-galactosidase provides visual screening of recombinant virus plaques. Molecular and Cellular Biology 5:3403–3409
    [Google Scholar]
  4. Dragunova J., Kozuch O., Gresikova M. 1986; Phenotypic mixing between vesicular stomatitis and Uukuniemi viruses. ActaVirologica 30:512–514
    [Google Scholar]
  5. Elliott R. M. 1990; Molecular biology of the Bunyaviridae. Journal of General Virology 71:501–522
    [Google Scholar]
  6. González-Scarano F., Shope R. E., Calisher C. H., Nathanson N. 1982; Characterization of monoclonal antibodies against the G1 and N proteins of La Crosse and Tahyna, two California serogroup bunya-viruses. Virology 120:42–53
    [Google Scholar]
  7. González-Scarano F., Shope R. E., Calisher C. H., Nathanson N. 1983; Monoclonal antibodies against the G1 and nucleocapsid proteins of La Crosse and Tahyna viruses. In California Serogroup Viruses pp. 145–156 Calisher C. H., Thompson W. H. Edited by New York: Alan R. Liss;
    [Google Scholar]
  8. Grady L. J., Sanders M. L., Campbell W. P. 1987; The sequence of the M RNA of an isolate of La Crosse virus. Journal ofGeneral Virology 68:3057–3071
    [Google Scholar]
  9. Green E. M., Armstrong S. J., Dimmock N. J. 1992; Mechanisms of neutralization of a nairovirus (Dugbe virus) by polyclonal IgG and IgM. Journal of General Virology 73:1995–2001
    [Google Scholar]
  10. Griot C., González-Scarano F., Nathanson N. 1993; Molecular determinants of the virulence and infectivity of California serogroupbunyaviruses. Annual Review of Microbiology 47:117–138
    [Google Scholar]
  11. Hewlett M. J., Chiu W. 1991; Virion structure. In Bunyaviridae pp. 79–90 Kolakofsky D. Edited by Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  12. Icenogle J., Shiwen H., Duke G., Gilbert S., Rueckert R., Anderegg J. 1983; Neutralization of poliovirus by a monoclonal antibody: kinetics and stoichiometry. Virology 12 7:412–425
    [Google Scholar]
  13. Jacoby D. R. 1992 Molecular cloning and expression of the La Crosse virus M segment: implications for structure, processing, intracellular targeting and membrane fusion PhD thesis University of Pennsylvania, USA:
    [Google Scholar]
  14. Jacoby D. R., Cooke C., Prabakaran I., Boland J., Nathanson N., González-Scarano F. 1993; Expression of the La Crosse M segment proteins in a recombinant vaccinia expression system mediates pH-dependent cellular fusion. Virology 193:993–996
    [Google Scholar]
  15. Kingsford L., Boucquey K. H., Cardos T. P. 1991; Effects of specific monoclonal antibodies on La Crosse virus neutralization: aggregation, inactivation by Fab fragments, and inhibition of attachment to baby hamster kidney cells. Virology 180:591–601
    [Google Scholar]
  16. Landau N. R., Page K. A., Littman D. R. 1991; Pseudotyping with human T-cell leukemia virus type I broadens the human immunodeficiency virus host range. Journal of Virology 65:162–169
    [Google Scholar]
  17. Najjar J. A., Gentsch J. R., Nathanson N., González-Scarano F. 1985; Epitopes of the G1 glycoprotein of La Crosse virus form overlapping clusters within a single antigenic site. Virology 144:426–432
    [Google Scholar]
  18. Obijeski J. F., Bishop D. H. L., Murphy F. A., Palmer E. L. 1976; Structural proteins of La Crosse virus. Journal of Virology 19:985–997
    [Google Scholar]
  19. Pekosz A., Griot C., Nathanson N., González-Scarano F. 1995; Tropism of bunyaviruses: evidence for a G1 glycoprotein-mediated entry pathway common to the California serogroup. Virology 214:339–348
    [Google Scholar]
  20. Salzwedel K., Johnston P. B., Roberts S. J., Dubay J. W., Hunter E. 1993; Expression and characterization of glycophospholipid-anchored human immunodeficiency virus type 1 envelope glycoproteins. Journal of Virology 67:5279–5288
    [Google Scholar]
  21. Talmon Y., Prasad B. V. V., Clerx J. P. M., Wang G.-J., Chiu W., Hewlett M. J. 1987; Electron microscopy of vitrified-hydrated La Crosse virus. Journal of Virology 61:2319–2321
    [Google Scholar]
  22. Taylor H. P., Armstrong S. J., Dimmock N. J. 1987; Quantitative relationships between an influenza virus and neutralizing antibody. Virology 159:288–298
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-4-667
Loading
/content/journal/jgv/10.1099/0022-1317-79-4-667
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error