1887

Abstract

Rabbit haemorrhagic disease virus (RHDV) causes an important disease in rabbits. The virus capsid is composed of a single 60 kDa protein. The capsid protein gene was cloned in using the pET3 system, and the antigenic structure of RHDV VP60 was dissected using 11 monoclonal antibodies (MAbs) and 12 overlapping fragments of the protein expressed in Two antigenic regions were found. Ten out of the 11 MAbs recognized different discontinuous epitopes in the most immunodominant region of the viral capsid. This domain was located between residues 31 and 250 of the VP60 N terminus. The other MAb revealed the presence of an antigenic site within 102 aa of the C terminus. This MAb did not recognize the major cleavage product of the full-length 60 kDa protein. These results indicate that, in contrast to other caliciviruses such as Norwalk virus (NV), the 36 kDa cleavage product probably forms the N-terminal region of VP60. However, as in NV, the cleavage region appears to be the most immunodominant region.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-8-1901
1998-08-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/8/9714238.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-8-1901&mimeType=html&fmt=ahah

References

  1. Bartoloni A., Pizza M., Bigio M., Nucci D., Ashworth L. A., Irons L. I., Robinson A., Burns D., Manclark C., Sato H., Rappuoli R. 1988; Mapping of a protective epitope of Pertussis toxin by in vitro refolding of recombinant fragments. Bio/Technology 6:709–712
    [Google Scholar]
  2. Berke T., Golding B., Jiang X., Cubitt D., Wolfaardt M., Smith A., Matson D. O. 1997; Phylogenetic analysis of the caliciviruses. Journal of Medical Virology 52:419–424
    [Google Scholar]
  3. Capucci L., Frigoli G., Ronshold L., Lavazza A., Brocchi E., Rossi C. 1995; Antigenicity of the rabbit hemorrhagic disease virus studied by its reactivity with monoclonal antibodies. VirusResearch 37:221–238
    [Google Scholar]
  4. Chuma T., Le Blois H., Sánchez-Vizcaíno J. M., Díaz-Laviada M., Roy P. 1992; Expression of the major core antigen VP7 of African horsesickness virus by a recombinant baculovirus and its use as a group-specific diagnostic reagent. Journal of General Virology 73:925–931
    [Google Scholar]
  5. Clarke I. N., Lambden P. R. 1997; The molecular biology of caliciviruses. Journal of General Virology 78:291–301
    [Google Scholar]
  6. Granzow H., Weiland F., Strebelow H.-G., Liu C. M., Schirrmeier H. 1996; Rabbit hemorrhagic disease virus (RHDV): ultrastructure and biochemical studies of typical and core-like particles present in liver homogenates. VirusResearch 41:163–172
    [Google Scholar]
  7. Hardy M. E., White L. J., Ball J. M., Estes M. K. 1995; Specific proteolytic cleavage of recombinant Norwalk virus capsid protein. Journal of Virology 69:1693–1698
    [Google Scholar]
  8. Hardy M. E., Tanaka T. N., Kitamoto N., White L., Ball J. M., Jiang X., Estes M. 1996; Antigenic mapping of the recombinant Norwalk virus capsidprotein using monoclonal antibodies. Virology 217:252–261
    [Google Scholar]
  9. Laurent S., Vautherot J. F., Madelaine M.-F., Le Gall G., Rasschaert D. 1994; Recombinant rabbit hemorrhagic disease virus capsid protein expressed in baculovirus self-assembles into virus-like particles and induces protection. Journal of Virology 68:6794–6798
    [Google Scholar]
  10. Laurent S., Vautherot J. F., Le Gall G., Madelaine M.-F., Rasschaert D. 1997; Structural, antigenic and immunogenic relationships between European brown hare syndrome virus and rabbit haemorrhagic disease virus. Journal of General Virology 78:2803–2811
    [Google Scholar]
  11. Liu S. J., Xue H. P., Pu B. Q., Quian N. H. 1984; A new viral disease in rabbits. Animal Husbandry and Veterinary Medicine 16:253–255
    [Google Scholar]
  12. López de Turiso J. A., Cortés E., García J., Ranz A., Sanz A., Vela C., Casal I. 1991; Fine mapping of canine parvovirus B cell epitopes. Journal of General Virology 72:2445–2456
    [Google Scholar]
  13. Martínez-Torrecuadrada J. L., Casal J. I. 1995; Identification of a linear neutralization domain in the protein VP2 of African horsesickness virus. Virology 210:391–399
    [Google Scholar]
  14. Meyers G., Wirblich C., Thiel H.-J. 1991; Rabbit hemorrhagic disease virus: molecular cloning and nucleotide sequence of a calicivirus genome. Virology 184:664–676
    [Google Scholar]
  15. Milton I. D., Turner J., Teelan A., Gaskell R., Turner P. C., Carter M. J. 1992; Location of monoclonal antibody binding sites in the capsid protein of feline calicivirus. Journal of General Virology 73:2435–2439
    [Google Scholar]
  16. Nakane P. K., Kawaoi A. 1974; Peroxidase-labeled antibody: a new method of conjugation. Journal of Histochemistry and Cytochemistry 22:1084–1091
    [Google Scholar]
  17. Ohlinger V. F., Haas B., Ahl R., Weiland F. 1989; Die infektiöse hämorrhagische Krankheit des Kaninchen - eine durch ein Calicivirus verursachte Tierseuche. Tieraerztliche Umschau 44:284–294
    [Google Scholar]
  18. Ohlinger V. F., Haas B., Meyer G., Weiland F., Thiel H.-J. 1990; Identification and characterization of the virus causing rabbit hemorrhagic disease. Journal of Virology 64:3331–3336
    [Google Scholar]
  19. Plana-Durán J., Bastons M., Rodriguez M. J., Climent I., Cortés E., Vela C., Casal I. 1996; Oral immunization of rabbits with VP60 particles confers protection against rabbit hemorrhagic disease. Archives of Virology 141:1423–1436
    [Google Scholar]
  20. Prasad B. V. V., Rothnagel R., Jiang X., Estes M. K. 1994; Three dimensional structure of baculovirus-expressed Norwalk virus capsids. Journal of Virology 68:5117–5125
    [Google Scholar]
  21. Reid G., Rigby M. A., McDonald M., Hosie M. J., Neil J. C., Jarret O. 1991; Immunodiagnosis of feline immunodeficiency virus infection using recombinant viral p17 and p24. AIDS 5:1477–1483
    [Google Scholar]
  22. Sambrook J., Fritsch T. J., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Sanz A., Garcia-Barreno B., Nogal M. L., Viñuela E., Enjuanes L. 1985; Monoclonal antibodies specific for African swine fever virus proteins. Journal of Virology 54:199–206
    [Google Scholar]
  24. Seal B. S., Ridpath J. F., Mengeling W. L. 1993; Analysis of feline calicivirus capsid protein genes : identification of variable antigenic determinant regions of the protein. Journal of General Virology 74:2519–2524
    [Google Scholar]
  25. Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. 1990; Use of T7 RNA polymerase to direct expression of cloned genes. Methods in Enzymology 185:60–89
    [Google Scholar]
  26. Summers M. D., Smith G. E. 1987 A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures Texas Agricultural Experiment Station Bulletin 1555
    [Google Scholar]
  27. Thouvenin E., Laurent S., Madelaine M.-F., Rasschaert D., Vautherot J.-F., Hewat E. A. 1997; Bivalent binding of a neutralising antibody to a calicivirus involves the tortional flexibility of the antibody hinge. Journal of Molecular Biology 270:238–246
    [Google Scholar]
  28. Tohya Y., Yokohama N., Maeda K., Kawaguchi Y., Mikami T. 1997; Mapping of antigenic sites involved in neutralization on the capsid protein of feline calicivirus. Journal of General Virology 78:303–305
    [Google Scholar]
  29. Wirblich C., Meyers G., Ohlinger V. F., Capucci L., Eskens U., Haas B., Thiel H.-J. 1994; European brown hare syndrome virus: relationship to rabbit hemorrhagic disease virus and other caliciviruses. Journal of Virology 68:5164–5173
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-8-1901
Loading
/content/journal/jgv/10.1099/0022-1317-79-8-1901
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error