1887

Abstract

The location of the BcLFI promoter of Epstein-Barr virus (EBV) has been identified by primer extension, which indicates that the 1 site of the BcLFI mRNA is located at nucleotide 137676 of the EBV genome. According to deletion analysis, the region upstream from nucleotide 38 is not essential for transcription of BcLFI. A 23 bp region in the promoter, from nucleotide 38 to 16, was identified as necessary for regulating the expression of BcLFI, i.e. the promoter activity is activated by 12-O-tetradecanoylphorbol 13-acetate but is repressed by phosphonoacetic acid. The results presented also demonstrate that the oriLyt sequence in is essential for enhancing the expression of BcLF1.

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1998-08-01
2024-05-03
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References

  1. Baer R., Bankier A. T., Biggin M. D., Deninger P. L., Farrell P. J., Gibson T. J., Hatfull G., Hudson G. S., Satchwell S. C., Seguin C., Tuffnell P. S., Barrell B. G. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310:207–211
    [Google Scholar]
  2. Biggin M., Bodescot M., Perricaudet M., Farrell P. 1987; Epstein-Barr virus gene expression in P3HR1-superinfected Raji cells. Journal of Virology 61:3120–3132
    [Google Scholar]
  3. Datta A. K., Hood R. E. 1981; Mechanism of inhibition of Epstein-Barr virus replication by phosphonoformic acid. Virology 114:52–59
    [Google Scholar]
  4. de Wet J. R., Wood K. V., DeLuca M., Helinski D. R., Subramani S. 1987; Firefly luciferase gene: structure and expression in mammalian cells. Molecular and Cellular Biology 7:725–737
    [Google Scholar]
  5. Faggioni A., Zompetta C., Grimaldi S., Barile G., Frati L., Lazdins J. 1986; Calcium modulation activates Epstein-Barr virus genome in latently infected cells. Science 232:1554–1556
    [Google Scholar]
  6. Hardwick J. M., Lieberman P. M., Hayward S. D. 1988; A new Epstein-Barr virus transactivator, R, induces expression of a cytoplasmic early antigen. Journal of Virology 62:2274–2284
    [Google Scholar]
  7. Johnson P. A., Everett R. D. 1986; The control of herpes simplex virus type-1 late gene transcription: a ‘TATA-box’/cap site region is sufficient for fully efficient regulated activity. Nucleic Acids Research 14:8247–8264
    [Google Scholar]
  8. Luka J., Kallin B., Klein G. 1979; Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate. Virology 94:228–231
    [Google Scholar]
  9. Mills L. K., Shi Y., Millette R. T. 1994; YY1 is the cellular factor shown previously to bind to regulatory regions of several leaky-late (βγ, γ1) genes of herpes simplex virus type 1. Journal of Virology 68:1234–1238
    [Google Scholar]
  10. Rickinson A. B., Kieff E. 1996; Epstein -Barr virus. In Fields Virology, 3rd edn. pp. 2397–2446 Fields B. N., Knipe D. M., Howley P. M. Edited by Philadelphia: Lippincott-Raven;
    [Google Scholar]
  11. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  13. Serio T. R., Kolman J. L., Miller G. 1997; Late gene expression from the Epstein-Barr virus BcLF1 and BFRF3 promoter does not require DNA replication in cis . Journal of Virology 71:8726–8734
    [Google Scholar]
  14. Shi Y., Seto E., Chang L.-S., Shenk T. 1991; Transcriptional repression by YY1, a human GL1-Kuppel-related protein, and relief of repression by adenovirus E1a protein. Cell 67:377–388
    [Google Scholar]
  15. Sinclair A. J., Farrell P. J. 1992; Epstein -Barr virus transcription factors. Cell Growth and Differentiation 3:557–563
    [Google Scholar]
  16. Summers W. C., Klein G. 1976; Inhibition of Epstein-Barr virus DNA synthesis and late gene expression by phosphonoacetic acid. Journal of Virology 18:151–155
    [Google Scholar]
  17. Takada K., Ono Y. 1989; Synchronous and sequential activation of latently infected Epstein-Barr virus genomes. Journal of Virology 63:445–449
    [Google Scholar]
  18. zur Hausen H., O’Neill F. J., Freese U. K., Hecker E. 1978; Persisting oncogenic herpesvirus induced by tumor promoter TPA. Nature 272:373–375
    [Google Scholar]
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