1887

Abstract

Potyviral helper component-proteinase (HC-Pro) is a multifunctional protein involved in aphid transmission, long-distance movement, polyprotein processing, genome amplification and symptom expression. It has been proposed that the active form of HC-Pro is a dimer and that coat protein (CP)-HC-Pro interaction is required for aphid transmission. To test these proposed interactions between CP and HC-Pro of potato A potyvirus (PVA), the yeast two-hybrid system was used. HC-Pro was shown to interact with itself in yeast cells, as did CP. Taken together with previous observations, we conclude that the functional HC-Pro is a homodimer. Deletion analysis showed that a 24 aa domain in the N-terminal half and the C-terminal proteinase part of HC-Pro were required for the interaction between HC-Pro molecules. No interactions were found between HC-Pro and CP using the genes of aphid-transmissible as well as aphid non-transmissible strains of PVA.

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1999-05-01
2024-05-01
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References

  1. Atreya C. D., Pirone T. P. 1993; Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility. Proceedings of the National Academy of Sciences, USA 90:11919–11923
    [Google Scholar]
  2. Atreya P. L., Atreya C. D., Pirone T. P. 1991; Amino acid substitutions in the coat protein result in loss of insect transmissibility of a plant virus. Proceedings of the National Academy of Sciences, USA 88:7887–7891
    [Google Scholar]
  3. Blanc S., Lopez-Moya J. J., Wang R., Garcia-Lampasona S., Thorn-bury D. W., Pirone T. P. 1997; A specific interaction between coat protein and helper component correlates with aphid transmission of a potyvirus. Virology 231:141–147
    [Google Scholar]
  4. Carrington J. C., Cary S. M., Parks T. D., Dougherty W. G. 1989; A second proteinase encoded by a plant potyvirus genome. EMBO Journal 8:365–370
    [Google Scholar]
  5. Chen C. M., Kraut N., Groudine M., Weintraub H. 1996; I-mf, a novel myogenic repressor, interacts with members of the MyoD family. Cell 86:731–741
    [Google Scholar]
  6. Chien C.-T., Bartel P. L., Sternglanz R., Fields S. 1991; The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proceedings of the National Academy of Sciences, USA 88:9578–9582
    [Google Scholar]
  7. Cronin S., Verchot J., Haldeman-Cahill R., Schaad M. C., Carrington J. C. 1995; Long-distance movement factor: a transport function of the potyvirus helper component proteinase. Plant Cell 7:549–559
    [Google Scholar]
  8. Dolja V. V., Herndon K. L., Pirone T. P., Carrington J. C. 1993; Spontaneous mutagenesis of a plant potyvirus genome after insertion of a foreign gene. Journal of Virology 67:5968–5975
    [Google Scholar]
  9. Hollenberg S. M., Sternglanz R., Cheng P. F., Weintraub H. 1995; Identification of a new family of tissue-specific basic helix-loop-helix proteins with a two-hybrid system. Molecular and Cellular Biology 15:3813–3822
    [Google Scholar]
  10. Hong Y., Levay K., Murphy J. F., Klein P. G., Shaw J. G., Hunt A. G. 1995; A potyvirus polymerase interacts with the viral coat protein and VPg in yeast cells. Virology 214:159–166
    [Google Scholar]
  11. Huet H., Gal-On A., Meir E., Lecoq H., Raccah B. 1994; Mutations in the helper component protease gene of zucchini yellow mosaic virus affect its ability to mediate aphid transmissibility. Journal of General Virology 75:1407–1414
    [Google Scholar]
  12. Jagadish M. N., Ward C. W., Gough K. H., Tulloch P. A., Whittaker L. A., Shukla D. D. 1991; Expression of potyvirus coat protein in E. coli and yeast and its assembly into virus-like particles. Journal of General Virology 72:1543–1550
    [Google Scholar]
  13. Kasschau K. D., Carrington J. C. 1995; Requirement for HC-Pro processing during genome amplification of tobacco etch potyvirus. Virology 209:268–273
    [Google Scholar]
  14. Kasschau K. D., Cronin S., Carrington J. C. 1997; Genome amplification and long-distance movement functions associated with the central domain of tobacco etch potyvirus helper component-proteinase. Virology 228:251–262
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  16. Luban J., Alin K. B., Bossolt K. L., Humaran T., Goff S. P. 1992; Genetic assay for multimerization of retroviral gag polyproteins. Journal of Virology 66:5157–5160
    [Google Scholar]
  17. Maia I. G., Bernardi F. 1996; Nucleic acid-binding properties of a bacterially expressed potato virus Y helper component-proteinase. Journal of General Virology 77:869–877
    [Google Scholar]
  18. Maia I. G., Haenni A.-L., Bernardi F. 1996; Potyviral HC-Pro: a multifunctional protein. Journal of General Virology 77:1335–1341
    [Google Scholar]
  19. Peng Y. H., Kadoury D., Gal-On A., Huet H., Wang Y., Raccah B. 1998; Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: effects on aphid transmission and binding to purified virions. Journal of General Virology 79:897–904
    [Google Scholar]
  20. Pirone T. P., Blanc S. 1996; Helper-dependent vector transmission of plant viruses. Annual Review of Phytopathology 34:227–247
    [Google Scholar]
  21. Pruss G., Ge X., Shi X. M., Carrington J. C., Vance V. B. 1997; Plant viral synergism: the potyviral genome encodes a broad-range pathogenicity enhancer that transactivates replication of heterologous viruses. Plant Cell 9:859–868
    [Google Scholar]
  22. Puurand Ü., Mäkinen K., Paulin L., Saarma M. 1994; The nucleotide sequence of potato virus A genomic RNA and its sequence similarities with other potyviruses. Journal of General Virology 75:457–461
    [Google Scholar]
  23. Rajamäki M., Merits A., Rabenstein F., Andrejeva J., Paulin L., Kekarainen T., Kreuze J. F., Forster R. L. S., Valkonen J. P. T. 1998; Biological, serological, and molecular differences among isolates of potato A potyvirus. Phytopathology 88:311–321
    [Google Scholar]
  24. Riechmann J. L., Lain S., Garcia J. A. 1992; Highlights and prospects of potyvirus molecular biology. Journal of General Virology 73:1–16
    [Google Scholar]
  25. Rojas M. R., Zerbini F. M., Allison R. F., Gilbertson R. L., Lucas W. J. 1997; Capsid protein and helper component-proteinase function as potyvirus cell-to-cell movement proteins. Virology 237:283–295
    [Google Scholar]
  26. Salomon R., Bernardi F. 1995; Inhibition of viral aphid transmission by the N-terminus of the maize dwarf mosaic virus coat protein. Virology 213:676–679
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Schiestl R. H., Gietz R. D. 1989; High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Current Genetics 16:339–346
    [Google Scholar]
  29. Shi X. M., Miller H., Verchot J., Carrington J. C., Vance V. B. 1997; Mutations in the region encoding the central domain of helper component-proteinase (HC-Pro) eliminate potato virus X/potyviral synergism. Virology 231:35–42
    [Google Scholar]
  30. Thornbury D. W., Hellmann G. M., Rhoads R. E., Pirone T. P. 1985; Purification and characterization of potyvirus helper component. Virology 144:260–267
    [Google Scholar]
  31. Trawick J. D., Wright R. M., Poyton R. O. 1989; Transcription of yeast COX6, the gene for cytochrome c oxidase subunit VI, is dependent on heme and on the HAP2 gene. Journal of Biological Chemistry 264:7005–7008
    [Google Scholar]
  32. Valkonen J. P. T., Puurand Ü., Slack S. A., Mäkinen K., Saarma M. 1995; Three strain groups of potato A potyvirus based on hypersensitive responses in potato, serological properties, and coat protein sequences. Plant Disease 79:748–753
    [Google Scholar]
  33. Valkonen J. P. T., Puurand Ü., Rabenstein F., Guo D., Merits A., Rajamäki M., Sorri O., Saarma M. 1996; Studies on potato A potyvirus (PVA) using infectious transcripts from a full-length cDNA: elicitation of hypersensitive resistance responses in potato, and aphid-transmissibility. Xth International Congress of Virology, Jerusalem, Israel, August 11–16 Abstract no. 0967
    [Google Scholar]
  34. Vojtek B. A., Hollenberg S. M., Cooper J. A. 1993; Mammalian Ras interacts directly with the serine/threonine kinase Raf. Cell 174:205–214
    [Google Scholar]
  35. Wang R. Y., Powell G., Hardie J., Pirone T. P. 1998; Role of the helper component in vector-specific transmission of potyviruses. Journal of General Virology 79:1519–1524
    [Google Scholar]
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