1887

Journal of General Virology header logo

Volume 80, Issue 7

Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle Free

Abstract

Virus-specific T cell responses were measured in cattle seropositive for bovine viral diarrhoea virus (BVDV) and compared with those from BVDV-seronegative animals. CD4+ and CD8+ T cells were purified and co-cultured in vitro with autologous, BVDV-infected monocytes over a time-course to assess the kinetics of the proliferative response. Supernatants from parallel T cell cultures were harvested and the presence of the cytokines interleukin (IL)-2, IL-4 and interferon-gamma (IFN-gamma) were measured by ELISA (IFN-gamma) or by a bioassay (IL-2 and IL-4). CD4+ and CD8+ T cells from all seropositive, but not seronegative, cattle proliferated specifically in response to BVDV-infected monocytes. Measurement of cytokines in the supernatants from proliferating T cell cultures showed that the CD4+ T cell response was type 2-like, with extremely high levels of B cell growth factor and IL-4 activity together with comparatively low levels of IL-2 activity and IFN-gamma protein. The CD8+ T cell response, although more variable, appeared to be type 1-like, with increased IL-2 and IFN-gamma but no IL-4 or B cell stimulatory activity.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1999
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-7-1673
1999-07-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/7/0801673a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-7-1673&mimeType=html&fmt=ahah

References

  1. Alwan W. H., Openshaw P. J. M. 1993; Distinct patterns of T- and B-cell immunity to respiratory syncytial virus induced by individual viral proteins. Vaccine 11:431–437
     [Google Scholar]
  2. Arneborn P., Biberfeld G. 1983; T-lymphocyte subpopulations in relation to immunosuppression in measles and varicella. Infection and Immunity 39:29–37
     [Google Scholar]
  3. Beer M., Wolf G., Pichler J., Wolfmeyer A., Kaaden O.-R. 1997; Cytotoxic T-lymphocyte responses in cattle infected with bovine viral diarrhea virus. Veterinary Microbiology 58:9–22
     [Google Scholar]
  4. Bensaid A., Hadam M. 1991; Individual antigens of cattle. Bovine CD4 (BoCD4). Veterinary Immunology and Immunopathology 27:51–54
     [Google Scholar]
  5. Bielefeldt Ohmann H. 1988; BVD virus antigens in tissues of persistently viraemic, clinically normal cattle: implications for the pathogenesis of clinically fatal disease. Acta Veterinaria Scandanavica 29:77–84
     [Google Scholar]
  6. Bolin S. R., Sacks J. M., Crowder S. V. 1987; Frequency of association of noncytopathic bovine viral diarrhea virus with mononuclear leukocytes from persistently infected cattle. American Journal of Veterinary Research 48:1441–1445
     [Google Scholar]
  7. Brown G. B., Bolin S. R., Frank D. E., Roth J. A. 1991; Defective function of leukocytes from cattle persistently infected with bovine viral diarrhea virus, and the influence of recombinant cytokines. American Journal of Veterinary Research 52:381–387
     [Google Scholar]
  8. Brown W. C., Zhao S., Woods V. M., Tripp C. A., Tetzlaff C. L., Heussler V. T., Dobbelaere D. A. E., Rice-Ficht A. C. 1993; Identification of two Th1 cell epitopes on the Babesia bovis-encoded 77-kilodalton merozoite protein (Bb-1) by use of truncated recombinant fusion proteins. Infection and Immunity 61:236–244
     [Google Scholar]
  9. Brown W. C., Davis W. C., Dobbelaere D. A. E., Rice-Ficht A. C. 1994; CD4+ T-cell clones obtained from cattle chronically infected with Fasciola hepatica and specific for adult worm antigen express both unrestricted and Th2 cytokine profiles. Infection and Immunity 62:818–827
     [Google Scholar]
  10. Brownlie J., Clarke M. C., Howard C. J. 1984; Experimental production of fatal mucosal disease in cattle. Veterinary Record 114:535–536
     [Google Scholar]
  11. Brownlie J., Clarke M. C., Howard C. J., Pocock D. H. 1987; Pathogenesis and epidemiology of bovine virus diarrhoea virus infection of cattle. Annales de Recherches Veterinaires 18:157–166
     [Google Scholar]
  12. Canals A., Zarlenga D. S., Almeria S., Gasbarre L. C. 1997; Cytokine profile induced by a primary infection with Ostertagia ostertagi in cattle. Veterinary Immunology and Immunopathology 58:63–75
     [Google Scholar]
  13. Davis W. C., Brown W. C., Hamilton M. J., Wyatt C. R., Orden J. A., Khalid A. M., Naessens J. 1996; Analysis of monoclonal antibodies specific for the γδ TcR. Veterinary Immunology and Immunopathology 52:275–283
     [Google Scholar]
  14. Doherty P. C., Topham D. J., Tripp R. A., Cardin R. D., Brooks J. W., Stevenson P. G. 1997; Effector CD4+ and CD8+ T-cell mechanisms in the control of respiratory virus infections. Immunological Reviews 159:105–117
     [Google Scholar]
  15. Edwards S., Wood L., Hewitt-Taylor C., Drew T. W. 1986; Evidence for an immunocompromising effect of bovine pestivirus on bovid herpesvirus 1 vaccination. Veterinary Research Communications 10:297–302
     [Google Scholar]
  16. Fernandez A., Hewicker M., Trautwein G., Pohlenz J., Liess B. 1989; Viral antigen distribution in the central nervous system of cattle persistently infected with bovine viral diarrhea virus. Veterinary Pathology 26:26–32
     [Google Scholar]
  17. Gagnon S. J., Zeng W., Kurane I., Ennis F. A. 1996; Identification of two epitopes on the dengue 4 virus capsid protein recognized by a serotype-specific and a panel of serotype-cross-reactive human CD4+ cytotoxic T-lymphocyte clones. Journal of Virology 70:141–147
     [Google Scholar]
  18. Goddeeris B. M., Baldwin C. L., Ole-MoiYoi O., Morrison W. I. 1986; Improved methods for purification and depletion of monocytes from bovine peripheral blood mononuclear cells. Functional evaluation of monocytes in responses to lectins. Journal of Immunological Methods 89:165–173
     [Google Scholar]
  19. Griffin D. E., Ward B. J. 1993; Differential CD4 T cell activation in measles. Journal of Infectious Diseases 168:275–281
     [Google Scholar]
  20. Hancock G. E., Speelman D. J., Heers K., Bortell E., Smith J., Cosco C. 1996; Generation of atypical pulmonary inflammatory responses in BALB/c mice after immunization with native attachment (G) glycoprotein of respiratory syncytial virus. Journal of Virology 70:7783–7791
     [Google Scholar]
  21. Hooper L. B., Clarke M. C., Brownlie J. 1992; Lymphocyte proliferation in response to bovine viral diarrhoea virus antigen. Proceedings of the Second Symposium on Pestiviruses pp 131–135 Annecy, France: 1–3 October 1992
    [Google Scholar]
  22. Howard C. J., Clarke M. C., Brownlie J. 1985; An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to bovine viral diarrhoea virus (BVDV) in cattle sera. Veterinary Microbiology 10:359–369
     [Google Scholar]
  23. Howard C. J., Clarke M. C., Brownlie J. 1989; Protection against respiratory infection with bovine virus diarrhoea virus by passively acquired antibody. Veterinary Microbiology 19:195–203
     [Google Scholar]
  24. Howard C. J., Clarke M. C., Sopp P., Brownlie J. 1992; Immunity to bovine virus diarrhoea virus in calves: the role of different T-cell subpopulations analysed by specific depletion in vivo with monoclonal antibodies. Veterinary Immunology and Immunopathology 32:303–314
     [Google Scholar]
  25. Innocent G., Morrison I., Brownlie J., Gettinby G. 1997; A computer simulation of the transmission dynamics and the effects of duration of immunity and survival of persistently infected animals on the spread of bovine viral diarrhoea virus in dairy cattle. Epidemiology and Infection 119:91–100
     [Google Scholar]
  26. Johnson D. W., Muscoplat C. C. 1973; Immunologic abnormalities in calves with chronic bovine viral diarrhea. American Journal of Veterinary Research 34:1139–1141
     [Google Scholar]
  27. Kühnle G., Collins R. A., Scott J. E., Keil G. M. 1996; Bovine interleukins 2 and 4 expressed in recombinant bovine herpesvirus 1 are biologically active secreted glycoproteins. Journal of General Virology 77:2231–2240
     [Google Scholar]
  28. Lambot M., Douart A., Joris E., Letesson J. J., Pastoret P. P. 1997; Characterization of the immune response of cattle against non-cytopathic and cytopathic biotypes of bovine viral diarrhoea virus. Journal of General Virology 78:1041–1047
     [Google Scholar]
  29. Larsson B., Fossum C. 1992; Bovine virus diarrhoea virus induces in vitro a proliferative response of peripheral blood mononuclear cells from cattle immunized by infection. Veterinary Microbiology 31:317–325
     [Google Scholar]
  30. Liebler-Tenorio E. M., Greiser-Wilke I., Pohlenz J. F. 1997; Organ and tissue distribution of the antigen of the cytopathogenic bovine viral diarrhea virus in the early and advanced phase of experimental mucosal disease. Archives of Virology 142:1613–1634
     [Google Scholar]
  31. Livingston P. G., Kurane I., Dai L. C., Okamoto Y., Lai C. J., Men R., Karaki S., Takiguchi M., Ennis F. A. 1995; Dengue virus-specific, HLA-B35-restricted, human CD8+ cytotoxic T lymphocyte (CTL) clones. Recognition of NS3 amino acids 500 to 508 by CTL clones of two different serotype specificities. Journal of Immunology 154:1287–1295
     [Google Scholar]
  32. Lobigs M., Arthur C. E., Mullbacher A., Blanden R. V. 1994; The flavivirus nonstructural protein NS3 is a dominant source of cytotoxic T cell peptide determinants. Virology 202:195–201
     [Google Scholar]
  33. Lopez O. J., Osorio F. A., Kelling C. L., Donis R. O. 1993; Presence of bovine viral diarrhoea virus in lymphoid cell populations of persistently infected cattle. Journal of General Virology 74:925–929
     [Google Scholar]
  34. MacHugh N. D., Bensaid A., Howard C. J., Davis W. C., Morrison W. I. 1991; Analysis of the reactivity of anti-bovine CD8 monoclonal antibodies with cloned T cell lines and mouse L-cells transfected with bovine CD8. Veterinary Immunology and Immunopathology 27:169–172
     [Google Scholar]
  35. Naessens J., Sileghem M., MacHugh N., Park Y. H., Davis W. C., Toye P. 1992; Selection of BoCD25 monoclonal antibodies by screening mouse L cells transfected with the bovine p55-interleukin-2 (IL-2) receptor gene. Immunology 76:305–309
     [Google Scholar]
  36. Pauly T., Elbers K., König M., Lengsfeld T., Saalmüller A., Thiel H. J. 1995; Classical swine fever virus-specific cytotoxic T lymphocytes and identification of a T cell epitope. Journal of General Virology 76:3039–3049
     [Google Scholar]
  37. Potgieter L. N. D., McCracken M. D., Hopkins F. M., Walker R. D. 1984; Effect of bovine viral diarrhea virus infection on the distribution of infectious bovine rhinotracheitis virus in calves. American Journal of Veterinary Research 45:687–690
     [Google Scholar]
  38. Ramshaw I. A., Ramsay A. J., Karupiah G., Rolph M. S., Mahalingam S., Ruby J. C. 1997; Cytokines and immunity to viral infections. Immunological Reviews 159:119–135
     [Google Scholar]
  39. Sopp P., Hooper L. B., Clarke M. C., Howard C. J., Brownlie J. 1994; Detection of bovine viral diarrhoea virus p80 protein in subpopulations of bovine leukocytes. Journal of General Virology 75:1189–1194
     [Google Scholar]
  40. Westaway E. G., Brinton M. A., Gaidamovich S. Ya., Horzinek M. C., Igarashi A., Kaariainen L., Lvov D. K., Porterfield J. S., Russell P. K., Trent D. W. 1985; Togaviridae. Intervirology 24:125–139
     [Google Scholar]
  41. Whittle H. C., Dossetor J., Oduloju A., Bryceson A. D. M., Greenwood B. M. 1978; Cell-mediated immunity during natural measles infection. Journal of Clinical Investigation 62:678–684
     [Google Scholar]
  42. Williams D. J. L., Newson J., Naessens J. 1990; Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies. Veterinary Immunology and Immunopathology 24:267–283
     [Google Scholar]
  43. Woldehiwet Z., Hussin A. A. 1994; Cytotoxic T cell responses in lambs experimentally infected with Border disease virus. Veterinary Immunology and Immunopathology 41:201–209
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-7-1673
Loading
Differential cytokine responses of CD4+ and CD8+ T cells in response to bovine viral diarrhoea virus in cattle
J. Gen. Virol. 80, 1673 (1999); https://doi.org/10.1099/0022-1317-80-7-1673
/content/journal/jgv/10.1099/0022-1317-80-7-1673
/content/journal/jgv/10.1099/0022-1317-80-7-1673
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error