1887

Journal of General Virology header logo

Volume 81, Issue 10

Virus morphogenesis of nucleopolyhedrovirus in serum-free suspension culture Free

Abstract

single nucleopolyhedrovirus (HaSNPV) replication in serum-free suspension culture was studied in detail and the sequence of virus morphogenesis was determined by transmission electron microscopy. By 16 h post-infection (p.i.), virus replication was observed in the virogenic stroma by the appearance of nucleocapsids. Polyhedron formation was detected by 24 h p.i. and the polyhedron envelope (PE) was completely formed by 72 h p.i. PE morphogenesis of HaSNPV is significantly different compared to the extensively studied (Ac)MNPV. In AcMNPV-infected cells, fibrillar structures are found in both cytoplasm and nuclei, and the fibrillar structures in nuclei are in close association with maturing polyhedra during PE formation. Fibrillar structures that resemble the AcMNPV fibrillar structures were detected only in the cytoplasm of HaSNPV-infected cells and appeared to interact with calyx precursors there, but their role in PE formation is unclear. However, prominent calyx precursor structures of various shapes and sizes were observed in the nuclei of HaSNPV-infected cells as well, and they appeared to interact with polyhedra during PE formation. Both the calyx precursor structure and the cytoplasmic fibrillar structure were detected only after HaSNPV virion occlusion had started, indicating that they might have a role in formation of PE. Similar calyx precursor structures and cytoplasmic fibrillar structures were observed in both serum-supplemented and serum-free suspension cultures, as well as in HaSNPV-infected larval tissues, indicating that the structures observed are not cell culture artefacts.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-10-2531
2000-10-01
2024-05-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/10/0812531a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-10-2531&mimeType=html&fmt=ahah

References

  1. Adams J. R., Goodwin R. H., Wilcox T. A. 1977; Electron microscopic investigations on invasion and replication of insect baculoviruses in vivo and in vitro . Biologie Cellulaire 28:261–268
     [Google Scholar]
  2. Bergold G. H. 1963; The nature of nuclear polyhedrosis viruses. In Insect Pathology: An Advance Treatise pp 413–455 Edited by Steinhaus E. A. New York: Academic Press;
     [Google Scholar]
  3. Braunagel S. C., Summers M. D. 1994; Autographa californica nuclear polyhedrosis virus, PDV, and ECV viral envelopes and nucleocapsids: structural proteins, antigens, lipids and fatty acid profiles. Virology 202:315–328
     [Google Scholar]
  4. Carstens E. B., Tjia S. T., Doerfler W. 1979; Infection of Spodoptera frugiperda cells with Autographa californica nuclear polyhedrosis virus. Virology 99:386–398
     [Google Scholar]
  5. Chakraborty S., Kanhaisingh A., Greenfield P. F., Reid S., Monsour C. J., Teakle R. 1995; In vitro production of wild type Heliothis baculoviruses for use as biopesticides. Australasian Biotechnology 5:82–86
     [Google Scholar]
  6. Chakraborty S., Greenfield P. F., Reid S. 1996; In vitro production studies with a wild-type Helicoverpa baculovirus. Cytotechnology 22:217–224
     [Google Scholar]
  7. Chakraborty S., Monsour C., Teakle R., Reid S. 1999; Yield, biological activity, and field performance of a wild-type Helicoverpa Nucleopolyhedrovirus produced in Helicoverpa zea cell cultures. Journal of Invertebrate Pathology 73:199–205
     [Google Scholar]
  8. Chakraborty S., Reid S. 1999; Serial passage of a Helicoverpa armigera nucleopolyhedrovirus in Helicoverpa zea cell cultures. Journal of Invertebrate Pathology 73:303–308
     [Google Scholar]
  9. Christian P. 1994 Recombinant baculovirus insecticides: catalysts for a change of heart? In Biopesticides: Opportunities for Australian Industry Brisbane, Queensland: University of Queensland;
     [Google Scholar]
  10. Fitt G. P. 1994; Cotton pest management. Part 3. An Australian perspective. Annual Review of Entomology 39:543–562
     [Google Scholar]
  11. Fraser M. J. 1986; Ultrastructural observations of virion maturation in Autographa californica nuclear polyhedrosis virus infection Spodoptera frugiperda cell cultures. Journal of Ultrastructure and Molecular Structure Research 95:189–195
     [Google Scholar]
  12. Goodwin R. H., Vaughn J. L., Adams J. R., Louloudes S. J. 1973; The influence of insect cell lines and tissue-culture media on baculovirus polyhedra production. Miscellaneous Publication of the Entomological Society of America 9:66–72
     [Google Scholar]
  13. Granados R. R. 1978; Early events in the infection of Heliothis zea midgut cells by a baculovirus. Virology 90:170–174
     [Google Scholar]
  14. Granados R. R., Lawler K. A. 1981; In vivo pathway of Autographa californica baculovirus invasion and infection. Virology 108:297–308
     [Google Scholar]
  15. Granados R. R., Lawler K. A., Burand J. P. 1981; Replication of Heliothis zea baculovirus in an insect cell line. Intervirology 16:71–79
     [Google Scholar]
  16. Gross C. H., Russell R. L. Q., Rohrmann G. F. 1994; Orgyia pseudotsugata baculovirus p10 and polyhedron envelope protein genes: analysis of their relative expression levels and role in polyhedron structure. Journal of General Virology 75:1115–1123
     [Google Scholar]
  17. Harrap K. A. 1972a; The structure of nuclear polyhedrosis viruses. I. The inclusion body. Virology 50:114–123
     [Google Scholar]
  18. Harrap K. A. 1972b; The structure of nuclear polyhedrosis viruses. III. Virus assembly. Virology 50:133–139
     [Google Scholar]
  19. Hess R. T., Falcon L. A. 1978; Electron microscope observations of the membrane surrounding polyhedral inclusion bodies of insects. Archives of Virology 56:169–176
     [Google Scholar]
  20. Hughes P. R., Gettig R. R., McCarthy W. J. 1983; Comparison of the time–mortality response of Heliothis zea to 14 isolates of Heliothis nuclear polyhedrosis virus. Journal of Invertebrate Pathology 41:256–261
     [Google Scholar]
  21. Ignoffo C. M., Shapiro M., Hink W. F. 1971; Replication and serial passage of infectious Heliothis nucleopolyhedrosis virus in an established line of Heliothis zea cells. Journal of Invertebrate Pathology 18:131–134
     [Google Scholar]
  22. Kawamoto F., Suto C., Kumada N., Kobayashi M. 1977; Cytoplasmic budding of a nuclear polyhedrosis virus and comparative ultrastructural studies of envelopes. Microbiology and Immunology 21:255–265
     [Google Scholar]
  23. Knudson D. L., Harrap K. A. 1976; Replication of a nuclear polyhedrosis virus in a continuous cell culture of Spodoptera frugiperda : microscopy study of the sequence of events of the virus infection. Journal of Virology 17:254–268
     [Google Scholar]
  24. Lee S. Y., Poloumienko A., Belfry S., Chen W., MacAfree N., Morin B., Lucarotti C., Krause M. 1996; A common pathway for p10 and calyx proteins in progressive stages of polyhedron envelope assembly in AcMNPV infected Spodoptera frugiperda larvae. Archives of Virology 141:1247–1258
     [Google Scholar]
  25. Lenz C. J., McIntosh A. H., Mazzacano C., Monderloh U. 1991; Replication of Heliothis zea nuclear polyhedrosis virus in cloned cell lines. Journal of Invertebrate Pathology 57:227–233
     [Google Scholar]
  26. McGahan P., Lloyd R. J., Rynne K. P. 1991; The cost of Heliothis in Queensland crops. In QDAYS P. I. Conference and Workshop Series QC91006 pp 11–28
     [Google Scholar]
  27. McIntosh A. H., Ignoffo C. M. 1981; Replication and infectivity of the single-embedded nuclear polyhedrosis virus, baculovirus Heliothis , in homologous cell line. Journal of Invertebrate Pathology 37:258–264
     [Google Scholar]
  28. MacKinnon E. A., Henderson J. F., Stoltz D. B., Faulkner P. 1974; Morphogenesis of nuclear polyhedrosis virus under conditions of prolonged passage in vitro . Journal of Ultrastructural Research 49:419–435
     [Google Scholar]
  29. Minion F. C., Coons L. B., Broome J. R. 1979; Characterisation of the polyhedral envelope of nuclear polyhedrosis virus of Heliothis virescens . Journal of Invertebrate Pathology 34:303–307
     [Google Scholar]
  30. Nielsen L. K., Smyth G. K., Greenfield P. F. 1991; Hemacytometer cell count distributions: implications of non-Poisson behavior. Biotechnology Progress 7:560–563
     [Google Scholar]
  31. Rice W. C., McIntosh A. H., Ignoffo C. M. 1989; Yield and activity of the Heliothis zea single nuclear polyhedrosis virus propagated in cloned and uncloned lines of Heliothis cells. In Vitro Cellular and Developmental Biology 25:201–204
     [Google Scholar]
  32. Rohrmann G. F. 1992; Baculovirus structural proteins. Journal of General Virology 73:749–761
     [Google Scholar]
  33. Stoltz D. B., Pavan C., da Cunha A. B. 1973; Nuclear polyhedrosis virus: a possible example of de novo intranuclear membrane morphogenesis. Journal of General Virology 19:145–150
     [Google Scholar]
  34. Teakle R. E., Jensen J. M., Mulder J. C. 1985; Susceptibility of Heliothis armigera (Lepidoptera: Noctuidea) on sorghum to a nuclear polyhedrosis virus. Journal of Economic Entomology 78:1373–1378
     [Google Scholar]
  35. Tewari S. K., Datta R. K. 1996; Ultrastructural studies on nuclear polyhedrosis virus infecting silkworm, Bombyx mori L. Indian Journal of Experimental Biology 34:141–150
     [Google Scholar]
  36. van Oers M. M., Vlak J. M. 1997; The baculovirus 10 kDa protein. Journal of Invertebrate Pathology 70:1–17
     [Google Scholar]
  37. van Oers M. M., Flipsen J. T., Reusken C. B., Sliwinsky E. L., Goldbach R. W., Vlak J. M. 1993; Functional domains of the p10 protein of Autographa californica nuclear polyhedrosis virus. Journal of General Virology 74:563–574
     [Google Scholar]
  38. van Oers M. M., Hu Z., Arif B. M., van Strien E. A., van Lent J. W., Vlak J. M. 1998; The single-nucleocapsid nucleopolyhedrovirus of Buzura suppressaria encodes a P10 protein. Journal of General Virology 79:1553–1562
     [Google Scholar]
  39. Vlak J. M. 1992; The biology of baculovirus in vivo and in cultured insect cells. In Baculovirus and Recombinant Protein Processes pp 2–9 Edited by Vlak J. M., Schlaeger E.-J., Bernard A. R. Zürich, Switzerland: Editions Roche;
     [Google Scholar]
  40. White G. G., Murray D. A. H., Walton M. P. 1996 Workshop report: Heliothis management in Australia Brisbane, Australia: Cooperative Research Centre for Tropical Pest Management, Brisbane; Cooperative Research Centre for Sustainable Cotton Production, Narrabi;
  41. Whitt M. A., Manning J. S. 1988; A phosphorylated 34-kDa protein and a subpopulation of polyhedrin are thiol linked to carbohydrate layer surrounding a baculovirus occlusion body. Virology 163:33–42
     [Google Scholar]
  42. Williams G. V., Faulkner P. 1997; Cytological changes and viral morphogenesis during baculovirus infection. In The Baculoviruses pp 61–108 Edited by Miller L. K. New York: Plenum;
     [Google Scholar]
  43. Williams G. V., Rohel D. Z., Kuzio J., Faulkner P. 1989; A cytopathological investigation of Autographa californica nuclear polyhedrosis virus p10 gene function using insertion/deletion mutants. Journal of General Virology 70:187–202
     [Google Scholar]
  44. Wood H. A. 1980; Isolation and replication of an occlusion body-deficient mutant of the Autographa californica nuclear polyhedrosis virus. Virology 105:338–344
     [Google Scholar]
  45. Yamada K., Sherman K. E., Maramorosch K. 1982; Serial passage of Heliothis zea singly embedded nuclear polyhedrosis virus in a homologous cell line. Journal of Invertebrate Pathology 39:185–191
     [Google Scholar]
  46. Young J. C., MacKinnon E. A., Faulkner P. 1993; The architecture of the virogenic stroma in isolated nuclei of Spodoptera frugiperda cells in vitro infected by Autographa californica nuclear polyhedrosis virus. Journal of Structural Biology 110:141–153
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-10-2531
Loading
Virus morphogenesis of Helicoverpa armigera nucleopolyhedrovirus in Helicoverpa zea serum-free suspension culture
J. Gen. Virol. 81, 2531 (2000); https://doi.org/10.1099/0022-1317-81-10-2531
/content/journal/jgv/10.1099/0022-1317-81-10-2531
/content/journal/jgv/10.1099/0022-1317-81-10-2531
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error